Next Article in Journal
An Attenuated Zika Virus Encoding Non-Glycosylated Envelope (E) and Non-Structural Protein 1 (NS1) Confers Complete Protection against Lethal Challenge in a Mouse Model
Previous Article in Journal
The Cellular Immune Response to Rabies Vaccination: A Systematic Review
 
Search for Articles:
Title / Keyword
Author / Affiliation
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
Journals
Vaccines
Volume 7
Issue 3
10.3390/vaccines7030111
vaccines-logo
Submit to this Journal Review for this Journal Edit a Special Issue
► Article Menu

Article Menu

share announcement question_answer thumb_up
...
textsms
...
Article

Mutations in VP0 and 2C Proteins of Duck Hepatitis A Virus Type 3 Attenuate Viral Infection and Virulence

by
Xingjian Wen
1,2,3,†,
Jinlong Guo
1,2,3,†,
Di Sun
1,2,3,†,
Mingshu Wang
1,2,3,†,
Dian Cao
1,2,3,
Anchun Cheng
1,2,3,*,
Dekang Zhu
1,2,3,
Mafeng Liu
1,2,3,
Xinxin Zhao
1,2,3,
Qiao Yang
1,2,3,
Shun Chen
1,2,3,
Renyong Jia
1,2,3,
Ying Wu
1,2,3,
Shaqiu Zhang
1,2,3,
Sai Mao
1,2,3,
Xumin Ou
1,2,3,
Xiaoyue Chen
1,2,3,
Yanling Yu
1,2,3,
Ling Zhang
1,2,3,
Yunya Liu
1,2,3,
Bin Tian
1,3,
Leichang Pan
1,3 and
Mujeeb Ur Rehman
1,3add Show full author list remove Hide full author list
1
Institute of Preventive Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
2
Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
3
Key Laboratory of Animal Disease and Human Health of Sichuan Province, Sichuan Agricultural University, Chengdu 611130, Sichuan, China
*
Author to whom correspondence should be addressed.
†
These authors have contributed equally to this work.
Vaccines 2019, 7(3), 111; https://doi.org/10.3390/vaccines7030111
Received: 26 July 2019 / Revised: 22 August 2019 / Accepted: 9 September 2019 / Published: 11 September 2019
(This article belongs to the Section Veterinary Vaccines)
View Full-Text Download PDF
Browse Figures

Abstract

Duck hepatitis A virus (DHAV) is prevalent worldwide and has caused significant economic losses. As the predominant serotype in China, DHAV-3 has become a major challenge to the local duck industry. Here the genetics and pathogenesis of a virulent DHAV-3 strain and its embryo-passaged strain were assessed. There were only two amino acid substitutions (Y164N in VP0 protein and L71I in 2C protein) introduced during the adaptation process. The pathogenicity of these strains was further evaluated in vivo. Clinical signs, gross pathology, and histopathological analysis showed that the embryo-passaged strain was attenuated. Meanwhile, the viral RNA loads were significantly lower in the liver tissues of the ducklings infected with the attenuated strain. As expected, infection with the virulent and attenuated strains led to the activation of different innate immune genes. We suspected that the loss of replication efficiency in ducklings was responsible for the attenuation phenotype of the embryo-passaged strain. In addition, different innate immune responses in the liver of ducklings were at least partly responsible for the differential infectivity phenotype. These findings provide new insights into the genetics and pathogenesis of DHAV-3, which may aid the development of new vaccines and the implementation of immunization strategies. View Full-Text
Keywords: duck hepatitis A virus; genetics; pathogenicity; attenuation; hepatic injury; innate immune response; intense inflammation; cytokine duck hepatitis A virus; genetics; pathogenicity; attenuation; hepatic injury; innate immune response; intense inflammation; cytokine
►▼ Show Figures

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited
SciFeed

Share and Cite

MDPI and ACS Style

Wen, X.; Guo, J.; Sun, D.; Wang, M.; Cao, D.; Cheng, A.; Zhu, D.; Liu, M.; Zhao, X.; Yang, Q.; Chen, S.; Jia, R.; Wu, Y.; Zhang, S.; Mao, S.; Ou, X.; Chen, X.; Yu, Y.; Zhang, L.; Liu, Y.; Tian, B.; Pan, L.; Rehman, M.U. Mutations in VP0 and 2C Proteins of Duck Hepatitis A Virus Type 3 Attenuate Viral Infection and Virulence. Vaccines 2019, 7, 111. https://doi.org/10.3390/vaccines7030111

AMA Style

Wen X, Guo J, Sun D, Wang M, Cao D, Cheng A, Zhu D, Liu M, Zhao X, Yang Q, Chen S, Jia R, Wu Y, Zhang S, Mao S, Ou X, Chen X, Yu Y, Zhang L, Liu Y, Tian B, Pan L, Rehman MU. Mutations in VP0 and 2C Proteins of Duck Hepatitis A Virus Type 3 Attenuate Viral Infection and Virulence. Vaccines. 2019; 7(3):111. https://doi.org/10.3390/vaccines7030111

Chicago/Turabian Style

Wen, Xingjian, Jinlong Guo, Di Sun, Mingshu Wang, Dian Cao, Anchun Cheng, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Shun Chen, Renyong Jia, Ying Wu, Shaqiu Zhang, Sai Mao, Xumin Ou, Xiaoyue Chen, Yanling Yu, Ling Zhang, Yunya Liu, Bin Tian, Leichang Pan, and Mujeeb U. Rehman. 2019. "Mutations in VP0 and 2C Proteins of Duck Hepatitis A Virus Type 3 Attenuate Viral Infection and Virulence" Vaccines 7, no. 3: 111. https://doi.org/10.3390/vaccines7030111

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Article Access Map by Country/Region

1
Back to TopTop