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Vaccines 2018, 6(3), 41; https://doi.org/10.3390/vaccines6030041

Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques

Department of Pharmaceutical Sciences, College of Pharmacy, University of New Mexico Health Sciences Center, Albuquerque, NM 87102, USA
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 31 May 2018 / Revised: 29 June 2018 / Accepted: 6 July 2018 / Published: 10 July 2018
(This article belongs to the Special Issue Microparticles-based Vaccines)
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Abstract

Pulmonary delivery of drugs and vaccines is an established route of administration, with particulate-based carriers becoming an attractive strategy to enhance the benefits of pulmonary therapeutic delivery. Despite the increasing number of publications using the pulmonary route of delivery, the lack of effective and uniform administration techniques in preclinical models generally results in poor translational success. In this study, we used the IVIS Spectrum small-animal in vivo imaging system to compare the respiratory tract deposition and distribution pattern of a microsphere suspension (5 µm) in mice after 1, 4, and 24 h when delivered by oropharyngeal aspiration, the Microsprayer® Aerosolizer, and the BioLite Intubation System, three-widely reported preclinical inhalation techniques. We saw no significant differences in microsphere deposition in whole body images and excised lungs (at 1, 4, and 24 h); however, the three-dimensional (3D) images showed more localized deposition in the lungs with the MicroSprayer® and BioLite delivery techniques. Further, oropharyngeal aspiration (at 1 h) showed microsphere deposition in the oral cavity, in contrast to the MicroSprayer® and BioLite systems. The studies shown here will allow researchers to choose the appropriate pulmonary delivery method in animal models based on their study requirements. View Full-Text
Keywords: pulmonary delivery; microparticles; vaccines; respiratory tract deposition; IVIS; MicroSprayer® Aerosolizer; BioLite intubation; oropharyngeal aspiration pulmonary delivery; microparticles; vaccines; respiratory tract deposition; IVIS; MicroSprayer® Aerosolizer; BioLite intubation; oropharyngeal aspiration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Kunda, N.K.; Price, D.N.; Muttil, P. Respiratory Tract Deposition and Distribution Pattern of Microparticles in Mice Using Different Pulmonary Delivery Techniques. Vaccines 2018, 6, 41.

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