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Approaches and Perspectives for Development of African Swine Fever Virus Vaccines
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Mechanisms of Entry and Endosomal Pathway of African Swine Fever Virus

Virology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid (CSIC-UAM), 28049 Madrid, Spain
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Academic Editor: Alexander Zakhartchouk
Vaccines 2017, 5(4), 42; https://doi.org/10.3390/vaccines5040042
Received: 6 September 2017 / Revised: 19 October 2017 / Accepted: 31 October 2017 / Published: 8 November 2017
African Swine Fever Virus (ASFV) causes a serious swine disease that is endemic in Africa and Sardinia and presently spreading in Russia and neighboring countries, including Poland and recently, the Czech Republic. This uncontrolled dissemination is a world-wide threat, as no specific protection or vaccine is available. ASFV is a very complex icosahedral, enveloped virus about 200 nm in diameter, which infects several members of pigs. The virus enters host cells by receptor-mediated endocytosis that depends on energy, vacuolar pH and temperature. The specific receptor(s) and attachment factor(s) involved in viral entry are still unknown, although macropinocytosis and clathrin-dependent mechanisms have been proposed. After internalization, ASFV traffics through the endolysosomal system. The capsid and inner envelope are found in early endosomes or macropinosomes early after infection, colocalizing with EEA1 and Rab5, while at later times they co-localize with markers of late endosomes and lysosomes, such as Rab7 or Lamp 1. A direct relationship has been established between the maturity of the endosomal pathway and the progression of infection in the cell. Finally, ASFV uncoating first involves the loss of the outer capsid layers, and later fusion of the inner membrane with endosomes, releasing the nude core into the cytosol. View Full-Text
Keywords: ASFV; viral entry; macropinocytosis; CME; endosomes; signaling; PI3K; Rac-1; Pak-1; macrophages; uncoating ASFV; viral entry; macropinocytosis; CME; endosomes; signaling; PI3K; Rac-1; Pak-1; macrophages; uncoating
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Sánchez, E.G.; Pérez-Núñez, D.; Revilla, Y. Mechanisms of Entry and Endosomal Pathway of African Swine Fever Virus. Vaccines 2017, 5, 42.

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