Next Article in Journal
DNA Vaccines: Recent Developments and the Future
Previous Article in Journal
Vaccinia Virus LC16m8∆ as a Vaccine Vector for Clinical Applications
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Vaccines
Volume 2
Issue 4
10.3390/vaccines2040772
vaccines-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague

by
Tonie E. Rocke
1,*,
Brock Kingstad-Bakke
2,
Willy Berlier
2 and
Jorge E. Osorio
2
1
National Wildlife Health Center, U.S. Geological Survey, 6006 Schroeder Rd., Madison, WI 53711, USA
2
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison, WI 53706, USA
*
Author to whom correspondence should be addressed.
Vaccines 2014, 2(4), 772-784; https://doi.org/10.3390/vaccines2040772
Submission received: 28 May 2014 / Revised: 6 September 2014 / Accepted: 28 September 2014 / Published: 27 October 2014
Browse Figures
Versions Notes

Abstract

In previous studies, we demonstrated in mice and prairie dogs that simultaneous administration of two recombinant raccoon poxviruses (rRCN) expressing Yersinia pestis antigens (F1 and V307—a truncated version of the V protein) provided superior protection against plague challenge compared to individual single antigen constructs. To reduce costs of vaccine production and facilitate implementation of a sylvatic plague vaccine (SPV) control program for prairie dogs, a dual antigen construct is more desirable. Here we report the construction and characterization of a novel RCN-vectored vaccine that simultaneously expresses both F1 and V307 antigens. This dual antigen vaccine provided similar levels of protection against plague in both mice and prairie dogs as compared to simultaneous administration of the two single antigen constructs and was also shown to protect mice against an F1 negative strain of Y. pestis. The equivalent safety, immunogenicity and efficacy profile of the dual RCN-F1/V307 construct warrants further evaluation in field efficacy studies in sylvatic plague endemic areas.
Keywords: raccoon poxvirus; Yersinia pestis; plague; vaccine; prairie dogs raccoon poxvirus; Yersinia pestis; plague; vaccine; prairie dogs
Graphical Abstract

Share and Cite

MDPI and ACS Style

Rocke, T.E.; Kingstad-Bakke, B.; Berlier, W.; Osorio, J.E. A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague. Vaccines 2014, 2, 772-784. https://doi.org/10.3390/vaccines2040772

AMA Style

Rocke TE, Kingstad-Bakke B, Berlier W, Osorio JE. A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague. Vaccines. 2014; 2(4):772-784. https://doi.org/10.3390/vaccines2040772

Chicago/Turabian Style

Rocke, Tonie E., Brock Kingstad-Bakke, Willy Berlier, and Jorge E. Osorio. 2014. "A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague" Vaccines 2, no. 4: 772-784. https://doi.org/10.3390/vaccines2040772

APA Style

Rocke, T. E., Kingstad-Bakke, B., Berlier, W., & Osorio, J. E. (2014). A Recombinant Raccoon Poxvirus Vaccine Expressing both Yersinia pestis F1 and Truncated V Antigens Protects Animals against Lethal Plague. Vaccines, 2(4), 772-784. https://doi.org/10.3390/vaccines2040772

Article Metrics

Back to TopTop