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Synthetic Peptides Elicit Humoral Response against Porcine Reproductive and Respiratory Syndrome Virus in Swine

Francisco Perez-Duran
Fernando Calderon-Rico
Luis Enrique Franco-Correa
Alicia Gabriela Zamora-Aviles
Roberto Ortega-Flores
Daniel Durand-Herrera
Alejandro Bravo-Patiño
Ricarda Cortes-Vieyra
Ilane Hernandez-Morales
2 and
Rosa Elvira Nuñez-Anita
Facultad de Medicina Veterinaria y Zootecnia, Universidad Michoacana de San Nicolas de Hidalgo, Km. 9.5 S/N carretera Morelia-Zinapecuaro, La Palma, Tarimbaro CP 58893, Mexico
Laboratorio de Investigacion Interdisciplinaria, Escuela Nacional de Estudios Superiores Unidad Leon, Universidad Nacional Autonoma de Mexico, Blv. UNAM No. 2011, Leon, Guanajuato CP 37684, Mexico
Author to whom correspondence should be addressed.
Vaccines 2024, 12(6), 652;
Submission received: 7 May 2024 / Revised: 6 June 2024 / Accepted: 7 June 2024 / Published: 11 June 2024
(This article belongs to the Section Veterinary Vaccines)


The aim of this study was to analyze the immunogenic response elicited in swine by two synthetic peptides derived from GP5 to understand the role of lineal B epitopes in the humoral and B-cell-mediated response against the porcine reproductive and respiratory syndrome virus (PRRSV). For inoculation, twenty-one-day-old pigs were allocated into six groups: control, vehicle, vaccinated (Ingelvac-PRRSV, MLV®), non-vaccinated and naturally infected, GP5-B and GP5-B3. At 2 days post-immunization (dpi), the GP5-B3 peptide increased the serum concentrations of cytokines associated with activate adaptive cellular immunity, IL-1β (1.15 ± 1.15 to 10.17 ± 0.94 pg/mL) and IL-12 (323.8 ± 23.3 to 778.5 ± 58.11 pg/mL), compared to the control group. The concentration of IgGs anti-GP5-B increased in both cases at 21 and 42 dpi compared to that at 0 days (128.3 ± 8.34 ng/mL to 231.9 ± 17.82 and 331 ± 14.86 ng/mL), while IgGs anti-GP5-B3 increased at 21 dpi (105.1 ± 19.06 to 178 ± 15.09 ng/mL) and remained at the same level until 42 dpi. Also, antibody-forming/Plasma B cells (CD2+/CD21−) increased in both cases (9.85 ± 0.7% to 13.67 ± 0.44 for GP5-B and 15.72 ± 1.27% for GP5-B3). Furthermore, primed B cells (CD2−/CD21+) from immunized pigs showed an increase in both cases (9.62 ± 1.5% to 24.51 ± 1.3 for GP5-B and 34 ± 2.39% for GP5-B3) at 42 dpi. Conversely the naïve B cells from immunized pigs decreased compared with the control group (8.84 ± 0.63% to 6.25 ± 0.66 for GP5-B and 5.78 ± 0.48% for GP5-B3). Importantly, both GP5-B and GP5-B3 peptides exhibited immunoreactivity against serum antibodies from the vaccinated group, as well as the non-vaccinated and naturally infected group. In conclusion, GP5-B and GP5-B3 peptides elicited immunogenicity mediated by antigen-specific IgGs and B cell activation.
Keywords: PRRS; B-cells; CD2+, CD21+, GP5 PRRS; B-cells; CD2+, CD21+, GP5

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MDPI and ACS Style

Perez-Duran, F.; Calderon-Rico, F.; Franco-Correa, L.E.; Zamora-Aviles, A.G.; Ortega-Flores, R.; Durand-Herrera, D.; Bravo-Patiño, A.; Cortes-Vieyra, R.; Hernandez-Morales, I.; Nuñez-Anita, R.E. Synthetic Peptides Elicit Humoral Response against Porcine Reproductive and Respiratory Syndrome Virus in Swine. Vaccines 2024, 12, 652.

AMA Style

Perez-Duran F, Calderon-Rico F, Franco-Correa LE, Zamora-Aviles AG, Ortega-Flores R, Durand-Herrera D, Bravo-Patiño A, Cortes-Vieyra R, Hernandez-Morales I, Nuñez-Anita RE. Synthetic Peptides Elicit Humoral Response against Porcine Reproductive and Respiratory Syndrome Virus in Swine. Vaccines. 2024; 12(6):652.

Chicago/Turabian Style

Perez-Duran, Francisco, Fernando Calderon-Rico, Luis Enrique Franco-Correa, Alicia Gabriela Zamora-Aviles, Roberto Ortega-Flores, Daniel Durand-Herrera, Alejandro Bravo-Patiño, Ricarda Cortes-Vieyra, Ilane Hernandez-Morales, and Rosa Elvira Nuñez-Anita. 2024. "Synthetic Peptides Elicit Humoral Response against Porcine Reproductive and Respiratory Syndrome Virus in Swine" Vaccines 12, no. 6: 652.

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