Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Human Subject Recruitment and Sampling
2.2. Evaluation of Anti-SARS-CoV-2 Antibodies
2.3. Antigen-Specific B Cell Detection
2.4. Activation Induce Markers (AIM) Assay
2.5. Flow Cytometry Staining and Acquisition
2.6. Intracellular Cytokine Staining (ICS) Assay
2.7. Statistical Analysis
3. Results
3.1. Kinetics of Circulating Anti-RBD Antibodies and Antigen-Specific B Cells during Breakthrough Infection in Subjects Vaccinated with Two Doses of SARS-CoV-2 mRNA Vaccine
3.2. Deep Phenotypic Characterisation of S- and RBD-Specific B Cells
3.3. Primary and Recall T Cell Immune Responses during Breakthrough Infection
3.4. Cytokine Production during Primary and Recall T Cell Responses
3.5. Deep Characterisation of the Phenotype and Functionality of Antigen-Specific CD4+ T Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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D’Orso, S.; Pirronello, M.; Verdiani, A.; Rossini, A.; Guerrera, G.; Picozza, M.; Sambucci, M.; Misiti, A.; De Marco, L.; Salvia, A.; et al. Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection. Vaccines 2023, 11, 1705. https://doi.org/10.3390/vaccines11111705
D’Orso S, Pirronello M, Verdiani A, Rossini A, Guerrera G, Picozza M, Sambucci M, Misiti A, De Marco L, Salvia A, et al. Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection. Vaccines. 2023; 11(11):1705. https://doi.org/10.3390/vaccines11111705
Chicago/Turabian StyleD’Orso, Silvia, Marta Pirronello, Alice Verdiani, Angelo Rossini, Gisella Guerrera, Mario Picozza, Manolo Sambucci, Andrea Misiti, Lorenzo De Marco, Antonino Salvia, and et al. 2023. "Primary and Recall Immune Responses to SARS-CoV-2 in Breakthrough Infection" Vaccines 11, no. 11: 1705. https://doi.org/10.3390/vaccines11111705