The Deletion of US3 Gene of Pseudorabies Virus (PRV) ΔgE/TK Strain Induces Increased Immunogenicity in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cells and Viruses
2.2. Construction of Recombinant Plasmid and Virus
2.3. Growth Characteristics of PRV ΔgE/TK/US3 Strain
2.4. Apoptosis
2.5. Swine Leukocyte Antigen Class I Molecule (SLA-I)
2.6. Pro-Inflammatory Cytokines
2.7. Pathogenic and Immunological Experiments in Mice
2.8. Statistical Analysis
3. Results
3.1. Generation of gE/TK/US3-Deleted Recombinant Virus
3.2. The Multiple Step Growth Curves of PRV Strains
3.3. PRV ΔgE/TK/US3 Strain Promotes Apoptosis
3.4. The Deletion of US3 Gene Upregulates the Transcriptional Level of SLA-I
3.5. PRV ΔgE/TK/US3 Strain Downregulates the Levels of Pro-Inflammatory Cytokines
3.6. The Safety and Immunogenicity of PRV ΔgE/TK/US3 in Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Conflicts of Interest
References
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Primers | Sequence(5′→3′) | Restriction Site | Expected Product/bp |
---|---|---|---|
US3L-F/R | F: CGGGGTACCGCGAGTCTGCGGGATGGT | Kpn Ⅰ | 658 |
R: CCCAAGCTTAGCGTCGAGGGCTTCTGG | Hind Ⅲ | ||
US3R-F/R | F: CCGGAATTCCTCAACAATGAAGTGGGCAAC | EcoR Ⅰ | 778 |
R: CGGACTAGTTCAGACTCCAGGCGGAAGAT | Spe Ⅰ | ||
EGFP-F/R | F: CCCAAGCTTATAACTTCGTATAGCATACATTATACGAAGTTATTTCCCATAGTAACGCCAATAG | Hind Ⅲ | 1620 |
R: CCGGAATTCATAACTTCGTATAATGTATGCTATACGAAGTTATGAAAGGACAGTGGGAGTG | EcoR Ⅰ | ||
US3-F/R | F: GGAGATGGGTCACCAAGAGG | / | 1617 |
R: CCGCCGTCAAAGAACCAG | / | ||
SLA-I qF/R | F: AAGTCAAGGAAACCGCACAG | / | 113 |
R: CAAGTAGCAGCCAAACATGC | / | ||
TNF-α qF/R | F:TGATCCGCGACGTGGAA | / | 72 |
R: ACCGCCTGGAGTTCTGGAA | / | ||
IL-1β qF/R | F: ACTCCTTAGTCCTCGGCCA | / | 99 |
R: CCATCAGAGGCAAGGAGGAA | / | ||
IL-6 qF/R | F: GAGGATACCACTCCCAACAGACC | / | 141 |
R: AAGTGCATCATCGTTGTTCATACA | / | ||
β-actin qF/R | F:TCCTGCGGCA TCCACGAAAC | / | 82 |
R: CCGTGTTGGCGTAGAGGTCCTTG | / | ||
GAPDH qF/R | F: AGGTCGGTGTGAACGGAT | / | 123 |
R: TGTAGACCATGTAGTTGAGG | / |
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Deng, M.-M.; Sun, Y.-W.; Ding, C.-M.; Xu, X.-Y.; Guo, Z.-Y.; Han, Z.-W.; Lv, C.-Z.; Qi, J.-K.; Li, Y.-T.; Yang, X.; et al. The Deletion of US3 Gene of Pseudorabies Virus (PRV) ΔgE/TK Strain Induces Increased Immunogenicity in Mice. Vaccines 2022, 10, 1603. https://doi.org/10.3390/vaccines10101603
Deng M-M, Sun Y-W, Ding C-M, Xu X-Y, Guo Z-Y, Han Z-W, Lv C-Z, Qi J-K, Li Y-T, Yang X, et al. The Deletion of US3 Gene of Pseudorabies Virus (PRV) ΔgE/TK Strain Induces Increased Immunogenicity in Mice. Vaccines. 2022; 10(10):1603. https://doi.org/10.3390/vaccines10101603
Chicago/Turabian StyleDeng, Meng-Meng, Ya-Wei Sun, Chen-Meng Ding, Xi-Ya Xu, Zi-Yi Guo, Zi-Wei Han, Chen-Zhe Lv, Jiang-Kun Qi, Yong-Tao Li, Xia Yang, and et al. 2022. "The Deletion of US3 Gene of Pseudorabies Virus (PRV) ΔgE/TK Strain Induces Increased Immunogenicity in Mice" Vaccines 10, no. 10: 1603. https://doi.org/10.3390/vaccines10101603
APA StyleDeng, M.-M., Sun, Y.-W., Ding, C.-M., Xu, X.-Y., Guo, Z.-Y., Han, Z.-W., Lv, C.-Z., Qi, J.-K., Li, Y.-T., Yang, X., Yu, L.-Y., & Chen, L. (2022). The Deletion of US3 Gene of Pseudorabies Virus (PRV) ΔgE/TK Strain Induces Increased Immunogenicity in Mice. Vaccines, 10(10), 1603. https://doi.org/10.3390/vaccines10101603