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Open AccessArticle

Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module

1
Department of Biophysics, Faculty of Biology, Lomonosov Moscow State University, 119991 Moscow, Russia
2
A.N. Bach Institute of Biochemistry, Federal Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia
3
Faculty of Basic Medicine, MV Lomonosov Moscow State University, 117192 Moscow, Russia
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Center for Strategic Planning and Management of Medical and Biological Health Risks, 119121 Moscow, Russia
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Institute of Gene Biology, Russian Academy of Sciences, 119334 Moscow, Russia
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Institute of Cytology of the Russian Academy of Sciences, 194064 St. Petersburg, Russia
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M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997 Moscow, Russia
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A.M. Prokhorov General Physics Institute, Russian Academy of Sciences, 119991 Moscow, Russia
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Institute of Chemistry PC 14, Department of Bioenergetics, Technische Universität Berlin, 10623 Berlin, Germany
*
Author to whom correspondence should be addressed.
Antioxidants 2020, 9(9), 869; https://doi.org/10.3390/antiox9090869
Received: 18 August 2020 / Revised: 9 September 2020 / Accepted: 10 September 2020 / Published: 15 September 2020
(This article belongs to the Special Issue Carotenoids, Oxidative Stress and Disease)
To counteract oxidative stress, antioxidants including carotenoids are highly promising, yet their exploitation is drastically limited by the poor bioavailability and fast photodestruction, whereas current delivery systems are far from being efficient. Here we demonstrate that the recently discovered nanometer-sized water-soluble carotenoprotein from Anabaena sp. PCC 7120 (termed AnaCTDH) transiently interacts with liposomes to efficiently extract carotenoids via carotenoid-mediated homodimerization, yielding violet–purple protein samples. We characterize the spectroscopic properties of the obtained pigment–protein complexes and the thermodynamics of liposome–protein carotenoid transfer and demonstrate the delivery of carotenoid echinenone from AnaCTDH into liposomes with an efficiency of up to 70 ± 3%. Most importantly, we show efficient carotenoid delivery to membranes of mammalian cells, which provides protection from reactive oxygen species (ROS). Incubation of neuroblastoma cell line Tet21N in the presence of 1 μM AnaCTDH binding echinenone decreased antimycin A ROS production by 25% (p < 0.05). The described carotenoprotein may be considered as part of modular systems for the targeted antioxidant delivery. View Full-Text
Keywords: carotenoid; protein-protein interactions; carotenoid delivery; protein-membrane interactions; liposomes; oxidative stress carotenoid; protein-protein interactions; carotenoid delivery; protein-membrane interactions; liposomes; oxidative stress
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MDPI and ACS Style

Maksimov, E.G.; Zamaraev, A.V.; Parshina, E.Y.; Slonimskiy, Y.B.; Slastnikova, T.A.; Abdrakhmanov, A.A.; Babaev, P.A.; Efimova, S.S.; Ostroumova, O.S.; Stepanov, A.V.; Slutskaya, E.A.; Ryabova, A.V.; Friedrich, T.; Sluchanko, N.N. Soluble Cyanobacterial Carotenoprotein as a Robust Antioxidant Nanocarrier and Delivery Module. Antioxidants 2020, 9, 869.

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