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Open AccessArticle

Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective against Urinary Tract Bacteria

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Departamento de Nutrición y Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain
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Department of Technical and Soil Sciences, Faculty of Agriculture, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
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Axe Molécules d’Intérêt Biologique (MIB), Unité de Recherche Œnologie, EA4577, USC 1366 Institut National de la Recherche Agronomique (INRA), Institut des Sciences de la Vigne et du Vin (ISVV), Université de Bordeaux, 33882 Bordeaux, France
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Department of Food Science, Faculty of Food Science and Technology, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
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Department of Nephrology, University of Medicine and Pharmacy “Iuliu Hatieganu”, Victor Babeş 8, 400012 Cluj-Napoca, Romania
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Department of Clinical and Paraclinical Sciences, Faculty of Veterinary Medicine, University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Calea Mănăștur 3-5, 400372 Cluj-Napoca, Romania
*
Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(6), 478; https://doi.org/10.3390/antiox9060478
Received: 30 April 2020 / Revised: 27 May 2020 / Accepted: 1 June 2020 / Published: 2 June 2020
(This article belongs to the Special Issue Antioxidant capacity of Anthocyanins and Other Vegetal Pigments)
Anthocyanins are extensively studied for their health-related properties, including antibacterial activity against urinary tract infections (UTI). Among common fruits, blueberries, with their remarkable antioxidant capacity, are one of the richest sources. Anthocyanin-rich extracts were obtained from four varieties: Snowchaser, Star, Stella Blue and Cristina Blue, grown in the hot climate of Southern Spain. Their total anthocyanins contents (TAC) were determined spectrophotometrically, and the anthocyanin profile by ultra high performance liquid chromatography—tandem mass spectrometer (UHPLC-MS/MS). Their antioxidant activity was assessed by oxygen radical absorbance capacity (ORAC) assay, while antibacterial activity against strains isolated from UTI patients was assessed in vitro, helping to select the varieties with the highest bioactive potential. Star showed the highest TAC and antioxidant activity (1663 ± 159 mg of cyanidin-3-O-glucoside (cy-3-O-glu) equivalents/100 g fresh weight (FW), 6345 ± 601 μmol Trolox equivalents (TE)/100 g FW, respectively), followed by Cristina Blue, Stella Blue and Snowchaser. As far as we know, this is the first time that cyanidin-3-rutinoside has been identified in blueberries. The extracts inhibited all the tested strains, MICs ranging from 0.4 mg/mL (for Stella Blue extract against UTI P. aeruginosa) to 9.5 mg/mL (for all extracts against UTI K. pneumoniae ssp. pneumoniae). This is the first study that assessed in vitro the antibacterial activity of blueberries against Klebsiella pneumoniae, Providencia stuartii and Micrococcus spp. strains isolated from UTI. View Full-Text
Keywords: antibacterial activity; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Providencia stuartii; Micrococcus; cyanidin-3-rutinoside; ORAC; UHPLC-MS/MS Orbitrap antibacterial activity; Escherichia coli; Klebsiella pneumoniae; Pseudomonas aeruginosa; Providencia stuartii; Micrococcus; cyanidin-3-rutinoside; ORAC; UHPLC-MS/MS Orbitrap
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MDPI and ACS Style

Cerezo, A.B.; Cătunescu, G.M.; González, M. .-P.; Hornedo-Ortega, R.; Pop, C.R.; Rusu, C.C.; Chirilă, F.; Rotar, A.M.; Garcia-Parrilla, M.C.; Troncoso, A.M. Anthocyanins in Blueberries Grown in Hot Climate Exert Strong Antioxidant Activity and May Be Effective against Urinary Tract Bacteria. Antioxidants 2020, 9, 478.

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