Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells

Di Meo, Francesco; Cuciniello, Rossana; Margarucci, Sabrina; Bergamo, Paolo; Petillo, Orsolina; Peluso, Gianfranco; Filosa, Stefania; Crispi, Stefania

doi:10.3390/antiox9040279

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Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells

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Institute of Biosciences and BioResources-UOS Naples CNR, Via P. Castellino, 111- 80131 Naples, Italy

Department of Biology, University of Naples Federico II, Complesso Universitario Monte Sant’Angelo Via Cinthia, 80126 Naples, Italy

Institute on Terrestrial Ecosystems (IRET) CNR, Via P. Castellino 111, 80131 Naples, Italy

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Institute of Food Science CNR, Via Roma, 64, 83100 Avellino, Italy

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IRCCS Neuromed, Localitá Camerelle, 86077 Pozzilli (IS), Italy

Authors to whom correspondence should be addressed.

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Co-first authors.

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Co-last authors.

Antioxidants 2020, 9(4), 279; https://doi.org/10.3390/antiox9040279

Received: 21 February 2020 / Revised: 20 March 2020 / Accepted: 25 March 2020 / Published: 26 March 2020

(This article belongs to the Special Issue Botanical Antioxidants and Neurological Diseases)

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Abstract

Oxidative stress has been associated to neuronal cell loss in neurodegenerative diseases. Neurons are post-mitotic cells that are very sensitive to oxidative stress—especially considering their limited capacity to be replaced. Therefore, reduction of oxidative stress, and inhibiting apoptosis, will potentially prevent neurodegeneration. In this study, we investigated the neuroprotective effect of Ginkgo biloba extract (EGb 761) against H₂O₂ induced apoptosis in SK-N-BE neuroblastoma cells. We analysed the molecular signalling pathway involved in the apoptotic cell death. H₂O₂ induced an increased acetylation of p53 lysine 382, a reduction in mitochondrial membrane potential, an increased BAX/Bcl-2 ratio and consequently increased Poly (ADP-ribose) polymerase (PARP) cleavage. All these effects were blocked by EGb 761 treatment. Thus, EGb 761, acting as intracellular antioxidant, protects neuroblastoma cells against activation of p53 mediated pathway and intrinsic mitochondrial apoptosis. Our results suggest that EGb 761, protecting against oxidative-stress induced apoptotic cell death, could potentially be used as nutraceutical for the prevention and treatment of neurodegenerative diseases. View Full-Text

Keywords: ginkgo biloba; oxidative stress; p53; apoptosis; neuroprotection; neuroblastoma cells

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MDPI and ACS Style

Di Meo, F.; Cuciniello, R.; Margarucci, S.; Bergamo, P.; Petillo, O.; Peluso, G.; Filosa, S.; Crispi, S. Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells. Antioxidants 2020, 9, 279. https://doi.org/10.3390/antiox9040279

AMA Style

Di Meo F, Cuciniello R, Margarucci S, Bergamo P, Petillo O, Peluso G, Filosa S, Crispi S. Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells. Antioxidants. 2020; 9(4):279. https://doi.org/10.3390/antiox9040279

Chicago/Turabian Style

Di Meo, Francesco, Rossana Cuciniello, Sabrina Margarucci, Paolo Bergamo, Orsolina Petillo, Gianfranco Peluso, Stefania Filosa, and Stefania Crispi. 2020. "Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells" Antioxidants 9, no. 4: 279. https://doi.org/10.3390/antiox9040279

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Ginkgo biloba Prevents Oxidative Stress-Induced Apoptosis Blocking p53 Activation in Neuroblastoma Cells

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