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Open AccessArticle

Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice

Division of Molecular Medicine, Ruđer Bošković Institute, 10000 Zagreb, Croatia
Division of Experimental Physics, Ruđer Bošković Institute, 10000 Zagreb, Croatia
Division of Materials Chemistry, Ruđer Bošković Institute,10000 Zagreb, Croatia
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Antioxidants 2020, 9(2), 174;
Received: 7 February 2020 / Revised: 17 February 2020 / Accepted: 18 February 2020 / Published: 20 February 2020
(This article belongs to the Special Issue Modulators of Oxidative Stress: Chemical and Pharmacological Aspects)
Metabolic homeostasis is differently regulated in males and females. Little is known about the mitochondrial Sirtuin 3 (Sirt3) protein in the context of sex-related differences in the development of metabolic dysregulation. To test our hypothesis that the role of Sirt3 in response to a high-fat diet (HFD) is sex-related, we measured metabolic, antioxidative, and mitochondrial parameters in the liver of Sirt3 wild-type (WT) and knockout (KO) mice of both sexes fed with a standard or HFD for ten weeks. We found that the combined effect of Sirt3 and an HFD was evident in more parameters in males (lipid content, glucose uptake, pparγ, cyp2e1, cyp4a14, Nrf2, MnSOD activity) than in females (protein damage and mitochondrial respiration), pointing towards a higher reliance of males on the effect of Sirt3 against HFD-induced metabolic dysregulation. The male-specific effects of an HFD also include reduced Sirt3 expression in WT and alleviated lipid accumulation and reduced glucose uptake in KO mice. In females, with a generally higher expression of genes involved in lipid homeostasis, either the HFD or Sirt3 depletion compromised mitochondrial respiration and increased protein oxidative damage. This work presents new insights into sex-related differences in the various physiological parameters with respect to nutritive excess and Sirt3. View Full-Text
Keywords: sirtuin 3; high fat diet; sex differences; mice; oxidative stress; metabolic stress sirtuin 3; high fat diet; sex differences; mice; oxidative stress; metabolic stress
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Pinterić, M.; Podgorski, I.I.; Hadžija, M.P.; Tartaro Bujak, I.; Dekanić, A.; Bagarić, R.; Farkaš, V.; Sobočanec, S.; Balog, T. Role of Sirt3 in Differential Sex-Related Responses to a High-Fat Diet in Mice. Antioxidants 2020, 9, 174.

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