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Open AccessArticle

Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line

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Department of Biotechnology, Thiruvalluvar University, Vellore 632115, India
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Department of Pharmacology and Therapeutics, College of Medicine of Health Sciences, United Arab Emirates University, Al Ain 17666, UAE
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Department of Physics, Sri Padmavati Mahila Visvavidyalayam (Women University), Andhra Pradesh 517502, India
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Vellore Institute of Technology (VIT), School of Biological Sciences and Technology, Vellore 632104, India
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DST PURSE Centre, Sri Venkateswara University, Tirupati 517502, Andhra Pradesh, India
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Pharmacy School, Aqaba University of Technology, Aqaba 77110, Jordan
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Department of Plant protection, Faculty of Agriculture, University of Jordan, Amman 11942, Jordan
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Food Science and Technology Program, Beijing Normal University–Hong Kong Baptist University United International College, Zhuhai 519087, Guangdong, China
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Authors to whom correspondence should be addressed.
Antioxidants 2020, 9(1), 8; https://doi.org/10.3390/antiox9010008
Received: 17 October 2019 / Revised: 2 December 2019 / Accepted: 2 December 2019 / Published: 21 December 2019
(This article belongs to the Section Health Outcomes of Antioxidants and Oxidative Stress)
We synthesized the gold nanoparticles (AuNPs) using wedelolactone (WDL) and characterized them using UV-visible spectroscopy, fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopic (SEM), transmission electron microscopic (TEM), energy dispersive X-ray diffraction, and atomic force microscopic (AFM) studies. The electronic spectrum exhibited an absorption peak at 535 nm. The FT-IR results proved that WDL was stabilized on the surface of AuNPs by acting as a capping or reducing agent. The crystalline structure was affirmed by XRD pattern and the spherical shape of WDL-AuNPs was evidenced by SEM, TEM, and AFM. The synthesized WDL-AuNPS were evaluated for anti-diabetic activity in pancreatic RIN-5F cell lines. In vitro results showed that WDL-AuNPs did not only improve the insulin secretion affected by di-(2-ethylhexyl) phthalate (DEHP), but also the cell viability in RIN5F cells. WDL-AuNPs treatment modulates the pro-apoptotic proteins and anti-apoptotic proteins expression to prevent the cells undergoing apoptosis in DEHP-exposed RIN-5F cells. The exposure of DEHP causes an increase in ROS production and lipid peroxidation levels. The free radical scavenging and antioxidant properties of WDL-AuNPs increase the deleterious effect caused by DEHP. On the other side, WDL-AuNPs increase mRNA expressions of insulin-signaling proteins in RIN-5F cells. This study concludes that WDL-AuNPs can be successfully used to regulate the expression of Bcl-2 family proteins, reduce lipid peroxidation, and to improve the secretion of antioxidants and insulin through the GLUT2 pathway in RIN-5F cell lines. View Full-Text
Keywords: antidiabetic; wedelolactone; gold nanoparticles; di-(2-ethylhexyl) phthalate; RIN-5F cell line; cytotoxicity antidiabetic; wedelolactone; gold nanoparticles; di-(2-ethylhexyl) phthalate; RIN-5F cell line; cytotoxicity
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Ramachandran, V.; Arokia Vijaya Anand, M.; David, E.; Venkatachalam, K.; Vijayakumar, S.; Sankaran, V.; Balupillai, A.; Sangeetha, C.C.; Gothandam, K.M.; Kotakadi, V.S.; Ghidan, A.; Al Antary, T.; Xu, B. Antidiabetic Activity of Gold Nanoparticles Synthesized Using Wedelolactone in RIN-5F Cell Line. Antioxidants 2020, 9, 8.

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