The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells

Jakaria, Md.; Azam, Shofiul; Cho, Duk-Yeon; Haque, Md. Ezazul; Kim, In-Su; Choi, Dong-Kug

doi:10.3390/antiox8090348

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The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells

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Department of Applied Life Sciences and Integrated Bioscience, Graduate School, Konkuk University, Chungju 27478, Korea

Department of Integrated Bioscience and Biotechnology, College of Biomedical and Health Sciences, and Research Institute of Inflammatory Diseases (RID), Konkuk University, Chungju 27478, Korea

Author to whom correspondence should be addressed.

Antioxidants 2019, 8(9), 348; https://doi.org/10.3390/antiox8090348

Received: 8 July 2019 / Revised: 24 August 2019 / Accepted: 24 August 2019 / Published: 1 September 2019

(This article belongs to the Special Issue Botanical Antioxidants and Neurological Diseases)

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Abstract

Neuroinflammation, apoptosis, and oxidative stress are connected to the pathogenesis of neurodegenerative diseases (NDDs). Targeting these three factors, the intervention of neuroprotective agents may have great potential in the treatment of NDDs. In the current study, the anti-inflammatory effects of the methanol extract of Allium cepa (MEAC) in lipopolysaccharide (LPS)-induced BV-2 microglial cells were investigated. MEAC has been studied in regard to the regulation of the antiapoptotic gene (Bcl-2) and various antioxidant enzyme (HO-1, NQO-1, and catalase) expressions in N27-A cells. Additionally, the protective action of MEAC has also been studied against MPP⁺-induced death in N27-A cells. The results suggest that MEAC is significantly protected from NO release and increase iNOS expression at the mRNA and protein levels in LPS-stimulated BV-2 microglial cells. MEAC treatment also protects COX-2 expression at the mRNA and protein levels. Furthermore, MEAC treatment prevents LPS-stimulated increases of proinflammatory cytokines, including TNF-α, IL-6, and IL-1β. In N27-A cells, MEAC treatment significantly upregulates antiapoptotic gene (Bcl-2) and antioxidant enzyme (HO-1, NQO1, and catalase) expressions. Moreover, MEAC treatment protects against MPP⁺-induced death in N27-A cells. To conclude, A cepa extract takes protective action against LPS and MPP⁺, and upregulates the antioxidant enzymes that could potentially be used in the therapy of NDDs. View Full-Text

Keywords: Allium cepa; protection; neurodegenerative diseases; inflammatory markers; Bcl-2; antioxidant enzymes

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Jakaria, M.; Azam, S.; Cho, D.-Y.; Haque, M.E.; Kim, I.-S.; Choi, D.-K. The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells. Antioxidants 2019, 8, 348. https://doi.org/10.3390/antiox8090348

AMA Style

Jakaria M, Azam S, Cho D-Y, Haque ME, Kim I-S, Choi D-K. The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells. Antioxidants. 2019; 8(9):348. https://doi.org/10.3390/antiox8090348

Chicago/Turabian Style

Jakaria, Md., Shofiul Azam, Duk-Yeon Cho, Md. Ezazul Haque, In-Su Kim, and Dong-Kug Choi. 2019. "The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells" Antioxidants 8, no. 9: 348. https://doi.org/10.3390/antiox8090348

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The Methanol Extract of Allium cepa L. Protects Inflammatory Markers in LPS-Induced BV-2 Microglial Cells and Upregulates the Antiapoptotic Gene and Antioxidant Enzymes in N27-A Cells

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