Functional Characterization of a Rice Thioredoxin Protein OsTrxm and Its Cysteine Mutant Variant with Antifungal Activity
by
Seong-Cheol Park 1,†
, Il Ryong Kim 2,3,†, Jin-Young Kim 1, Yongjae Lee 4, Su-Hyang Yoo 2, Ji Hyun Jung 3,5, Gang-Won Cheong 6, Sang Yeol Lee 3, Mi-Kyeong Jang 1,7,* and Jung Ro Lee 2,*
Seong-Cheol Park 1,†, Il Ryong Kim 2,3,†, Jin-Young Kim 1, Yongjae Lee 4, Su-Hyang Yoo 2, Ji Hyun Jung 3,5, Gang-Won Cheong 6, Sang Yeol Lee 3, Mi-Kyeong Jang 1,7,* and Jung Ro Lee 2,*
1
Department of Polymer Science and Engineering, Sunchon National University, Suncheon 57922, Korea
2
Division of Ecological Safety Research, National Institute of Ecology, 1210 Geumgang-ro, Maseo-myeon, Seocheon 33657, Korea
3
Division of Applied Life Science (BK21+ Program), Plant Molecular Biology and Biotechnology Research Center (PMBBRC), Gyeongsang National University, Jinju 52828, Korea
4
Department of Nutrition and Food Science, Texas A&M University, College Station, TX 77843, USA
5
Goseong Agricultural Development/Technology Center, Goseong 52930, Korea
6
Division of Applied Life Sciences and Research Institute of Natural Science, Gyeongsang National University, Jinju 52828, Korea
7
The Research Institute for Sanitation and Environment of Coastal Areas, Sunchon National University, Suncheon 57922, Korea
*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Antioxidants 2019, 8(12), 598; https://doi.org/10.3390/antiox8120598
Received: 15 October 2019 / Revised: 18 November 2019 / Accepted: 26 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Thioredoxin Family Proteins)
Although there are many antimicrobial proteins in plants, they are not well-explored. Understanding the mechanism of action of plant antifungal proteins (AFPs) may help combat fungal infections that impact crop yields. In this study, we aimed to address this gap by screening Oryza sativa leaves to isolate novel AFPs. We identified a thioredoxin protein with antioxidant properties. Being ubiquitous, thioredoxins (Trxs) function in the redox balance of all living organisms. Sequencing by Edman degradation method revealed the AFP to be O. sativa Thioredoxin m-type isoform (OsTrxm). We purified the recombinant OsTrxm and its cysteine mutant proteins (OsTrxm C/S) in Escherichia coli. The recombinant OsTrxm proteins inhibited the growth of various pathogenic fungal cells. Interestingly, OsTrxm C/S mutant showed higher antifungal activity than OsTrxm. A growth inhibitory assay against various fungal pathogens and yeasts confirmed the pertinent role of cysteine residues. The OsTrxm protein variants penetrated the fungal cell wall and membrane, accumulated in the cells and generated reactive oxygen species. Although the role of OsTrxm in chloroplast development is known, its biochemical and molecular functions have not been elucidated. These findings suggest that in addition to redox regulation, OsTrxm also functions as an antimicrobial agent.
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Keywords:
OsTrxm; active cysteine; antifungal protein; rice; pathogen
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MDPI and ACS Style
Park, S.-C.; Kim, I.R.; Kim, J.-Y.; Lee, Y.; Yoo, S.-H.; Jung, J.H.; Cheong, G.-W.; Lee, S.Y.; Jang, M.-K.; Lee, J.R. Functional Characterization of a Rice Thioredoxin Protein OsTrxm and Its Cysteine Mutant Variant with Antifungal Activity. Antioxidants 2019, 8, 598.
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