Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts
Abstract
:1. Introduction
2. Experimental Section
2.1. General Information
2.2. Plant Material
2.3. Preparation of Extracts
2.4. Measurement of Antioxidant Activity
2.4.1. β-Carotene/Linoleic Acid Method
2.4.2. Scavenging of 1,1-Diphenyl-2-Picrylhydrazyl (DPPH)
2.4.3. ABTS+ Scavenging Activity
2.5. Concentrations of Constituents
2.5.1. Total Phenol Concentration
2.5.2. Total Flavonoid Concentration
2.5.3. Condensed Tannin Concentration (CT)
2.5.4. Flavonol Concentration
2.6. HPLC/PDA Analysis
3. Results and Discussion
3.1. Antioxidant Activity
Test | |||||
---|---|---|---|---|---|
DPPH | β-Carotene/Linoleic Acid | ABTS+ | |||
IC50 (μg/mL) (Limit Confidence 95%) | %FRS * | (%AA) | (%) | ||
Extracts | P. carthagenensis | 16.92 ± 4.58 | 93.52 ± 8.41 | 79.12 ± 3.70 | 92.5 ± 7.43 |
(12.03–22.71) | |||||
P. leiocarpa | 127.00 ± 10.55 | 54.13 ± 11.10 | 22.30 ± 7.14 | 12.20 ± 4.44 | |
(123.50–135.94) | |||||
P. capillacea | 30.05 ± 6.22 | 91.78 ± 4.23 | 33.40 ± 15.22 | 87.34 ± 8.32 | |
(26.68–37.27) | |||||
P. deflexa | 146.40 ± 12.47 | 66.37 ± 8.12 | 26.05 ± 10.60 | 15.58 ± 5.22 | |
(141.81–148.42) | |||||
Standards | BHT | 16.72 ± 1.34 | 92.19 ± 1.29 | 91.20 ± 4.54 | 96.4 ± 2.44 |
(14.08–17.22) | |||||
Ascorbic acid | 22.28 ± 0.53 | 96.40 ± 0.27 | 4.13 ± 1.42 | 80.9 ± 5.56 | |
(20.20–23.43) | |||||
Quercetin | n.d. | n.d. | 80.65 ± 1.25 | n.d. |
3.2. Levels of Constituents
3.3. HPLC/PAD Analysis from Psychotria Extracts
4. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Formagio, A.S.N.; Volobuff, C.R.F.; Santiago, M.; Cardoso, C.A.L.; Vieira, M.D.C.; Valdevina Pereira, Z. Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants 2014, 3, 745-757. https://doi.org/10.3390/antiox3040745
Formagio ASN, Volobuff CRF, Santiago M, Cardoso CAL, Vieira MDC, Valdevina Pereira Z. Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants. 2014; 3(4):745-757. https://doi.org/10.3390/antiox3040745
Chicago/Turabian StyleFormagio, Anelise Samara Nazari, Carla Roberta Ferreira Volobuff, Matheus Santiago, Claudia Andrea Lima Cardoso, Maria Do Carmo Vieira, and Zefa Valdevina Pereira. 2014. "Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts" Antioxidants 3, no. 4: 745-757. https://doi.org/10.3390/antiox3040745
APA StyleFormagio, A. S. N., Volobuff, C. R. F., Santiago, M., Cardoso, C. A. L., Vieira, M. D. C., & Valdevina Pereira, Z. (2014). Evaluation of Antioxidant Activity, Total Flavonoids, Tannins and Phenolic Compounds in Psychotria Leaf Extracts. Antioxidants, 3(4), 745-757. https://doi.org/10.3390/antiox3040745