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Antioxidants
Volume 14
Issue 12
10.3390/antiox14121403
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model

by
Thalisia Cunha dos Santos
1,2,*,
Johana Marcela Concha Obando
2,3,
Joana Silva
4,
Ana Luíza Vidal Pimentel Santos
5,
Roberto Carlos Campos Martins
1,
Diana Negrão Cavalcanti
6,
Rui Pedrosa
4 and
Celso Alves
4,*
1
Postgraduate Program in Chemistry of Natural Products, Instituto de Pesquisa em Produtos Naturais Walter Mors, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-599, Brazil
2
Seallg—Solutions, Innovation, and Products, Startup, Bioeconomy Living Lab and Science and Technology-Based, Startup Incubators in the Ribeira Valley (Aquário de Ideias), Registro 11900-000, Brazil
3
National Institute of Science and Technology (INCT) in Nanotechnology for Sustainable Agriculture (INCTNanoAgro), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Sorocaba 18087-180, Brazil
4
MARE-Marine and Environmental Sciences Centre & ARNET, ESTM, Polytechnic University of Leiria, 2520-630 Peniche, Portugal
5
Faculty of Pharmacy, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-853, Brazil
6
Postgraduate Program in Marine Biology and Coastal Ecosystems, Instituto de Biologia, Universidade Federal Fluminense, Niterói 24210-201, Brazil
*
Authors to whom correspondence should be addressed.
Antioxidants 2025, 14(12), 1403; https://doi.org/10.3390/antiox14121403
Submission received: 23 September 2025 / Revised: 14 November 2025 / Accepted: 16 November 2025 / Published: 25 November 2025
(This article belongs to the Special Issue Antioxidants from the Sea and Their Application)
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Abstract

Brazilian native seaweed Canistrocarpus cervicornis (Ochrophyta, Dictyotaceae) is recognized for its chemodiversity, particularly cyclic diterpenes and polysaccharides, yet its relevance to neurological disorders remains unexplored. This study evaluated the neuroprotective potential of a hydroethanolic extract (ECCH), its polar fraction (CCFPol), a dichloromethane extract (ECCD), and eight derived fractions (CCF1–2, CCF3, CCF4, CCF5–6, CCF7, CCF8–10, and CCF11–15). Cytotoxicity was evaluated in SH-SY5Y neuroblastoma cells, and neuroprotection was examined against 6-OHDA–induced toxicity. The mitochondrial membrane potential, ROS and H2O2 generation assays were conducted to explore the mechanisms underlying the observed effects. Among the key findings, the CCF3 fraction exhibited a high content (75.04%) of dolastane-type diterpenoids. Both CCFPol (100 µg/mL) and CCF3 (1 µg/mL) increased cell viability to 68.43 ± 4.60% and 60.61 ± 0.80%, respectively, compared with 6-OHDA–treated cells (50.70 ± 2.71%). Additionally, CCF3 and CCFPol reduced H2O2 levels (200.0 ± 18.19% and 195.5 ± 16.13%, respectively, vs. 6-OHDA-treated cells: 302.2 ± 17.07%) and lowered intracellular ROS (122.6 ± 22.7% and 129.6 ± 19.4%, respectively, vs. 6-OHDA-treated cells: 153.0 ± 32.7%). This is the first study to demonstrate the neuroprotective potential of the C. cervicornis in a 6-OHDA-induced neurotoxicity cellular model, contributing to the understanding of marine bioactive resources and their relevance for neurological research. Additional studies aimed at isolating the active constituents and clarifying their mechanisms of action will further strengthen and expand the biological relevance of this specie as source of neuroprotective agents.
Keywords: bioproducts; Dictyotaceae; neuroprotection; SH-SY5Y; neurological diseases; marine natural products; oxidative stress; mitochondrial disfunction; Apoptosis bioproducts; Dictyotaceae; neuroprotection; SH-SY5Y; neurological diseases; marine natural products; oxidative stress; mitochondrial disfunction; Apoptosis
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MDPI and ACS Style

dos Santos, T.C.; Obando, J.M.C.; Silva, J.; Santos, A.L.V.P.; Martins, R.C.C.; Cavalcanti, D.N.; Pedrosa, R.; Alves, C. Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model. Antioxidants 2025, 14, 1403. https://doi.org/10.3390/antiox14121403

AMA Style

dos Santos TC, Obando JMC, Silva J, Santos ALVP, Martins RCC, Cavalcanti DN, Pedrosa R, Alves C. Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model. Antioxidants. 2025; 14(12):1403. https://doi.org/10.3390/antiox14121403

Chicago/Turabian Style

dos Santos, Thalisia Cunha, Johana Marcela Concha Obando, Joana Silva, Ana Luíza Vidal Pimentel Santos, Roberto Carlos Campos Martins, Diana Negrão Cavalcanti, Rui Pedrosa, and Celso Alves. 2025. "Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model" Antioxidants 14, no. 12: 1403. https://doi.org/10.3390/antiox14121403

APA Style

dos Santos, T. C., Obando, J. M. C., Silva, J., Santos, A. L. V. P., Martins, R. C. C., Cavalcanti, D. N., Pedrosa, R., & Alves, C. (2025). Chemical Characterization and Bio-Screening of Neuroprotective Potential of Brazilian Brown Seaweed Canistrocarpus cervicornis in 6-OHDA-Induced Neurotoxicity Model. Antioxidants, 14(12), 1403. https://doi.org/10.3390/antiox14121403

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