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Antioxidants
Volume 13
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10.3390/antiox13101221
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Article

By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models

by
Valeria Scalcon
1,*,
Federico Fiorese
1,
Marica Albanesi
2,3,
Alessandra Folda
1,
Gianfranco Betti
4,
Marco Bellamio
5,
Emiliano Feller
5,
Claudia Lodovichi
2,3,6,
Giorgio Arrigoni
1,
Oriano Marin
1 and
Maria Pia Rigobello
1,*
1
Department of Biomedical Sciences, University of Padova, Via Ugo Bassi 58/b, 35132 Padova, Italy
2
Padova Neuroscience Center (PNC), University of Padova, Via Orus 2, 35129 Padova, Italy
3
Veneto Institute of Molecular Medicine (VIMM), Via Giuseppe Orus, 2, 35129 Padova, Italy
4
Centrale del Latte d’Italia S.p.A., Sede di Firenze, Via dell’Olmatello 20, 50127 Firenze, Italy
5
Centrale del Latte d’Italia S.p.A., Sede di Vicenza, Via Faedo 60, 36100 Vicenza, Italy
6
Institute of Neuroscience, Consiglio Nazionale delle Ricerche (CNR), Viale G. Colombo 3, 35121 Padova, Italy
*
Authors to whom correspondence should be addressed.
Antioxidants 2024, 13(10), 1221; https://doi.org/10.3390/antiox13101221
Submission received: 13 September 2024 / Revised: 7 October 2024 / Accepted: 8 October 2024 / Published: 10 October 2024
(This article belongs to the Special Issue Agri-Food Wastes as Natural Source of Bioactive Antioxidants—3rd Edition)
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Abstract

The discarding of agri-food by-products is a stringent problem due to their high environmental impact. Recovery strategies can lead to a reduction of waste and result in new applications. Agri-food waste represents a source of bioactive molecules, which could promote health benefits. The primary goal of this research has been the assessment of the antioxidant activity of milk permeate, a dairy farm by-product, and the isolation and identification of peptide fractions endowed with antioxidant activity. The chromatographic extraction of the peptide fractions was carried out, and the peptides were identified by mass spectrometry. The fractions showed radical scavenging activity in vitro. Moreover, the results in the Caco-2 cell model demonstrated that the peptide fractions were able to protect from oxidative stress by stimulating the Keap1/Nrf2 antioxidant signaling pathway, increasing the transcription of antioxidant enzymes. In addition, the bioactive peptides can affect cellular metabolism, increasing mitochondrial respiration. The action of the peptide fractions was also assessed in vivo on a zebrafish model and resulted in the protection of the whole organism from the adverse effects of acute cold stress, highlighting their strong capability to protect from an oxidative insult. Altogether, the results unveil novel recovery strategies for food by-products as sources of antioxidant bioactive peptides that might be utilized for the development of functional foods.
Keywords: circular economy; milk permeate; bioactive peptides; antioxidants; Caco-2 cells; zebrafish circular economy; milk permeate; bioactive peptides; antioxidants; Caco-2 cells; zebrafish
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MDPI and ACS Style

Scalcon, V.; Fiorese, F.; Albanesi, M.; Folda, A.; Betti, G.; Bellamio, M.; Feller, E.; Lodovichi, C.; Arrigoni, G.; Marin, O.; et al. By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models. Antioxidants 2024, 13, 1221. https://doi.org/10.3390/antiox13101221

AMA Style

Scalcon V, Fiorese F, Albanesi M, Folda A, Betti G, Bellamio M, Feller E, Lodovichi C, Arrigoni G, Marin O, et al. By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models. Antioxidants. 2024; 13(10):1221. https://doi.org/10.3390/antiox13101221

Chicago/Turabian Style

Scalcon, Valeria, Federico Fiorese, Marica Albanesi, Alessandra Folda, Gianfranco Betti, Marco Bellamio, Emiliano Feller, Claudia Lodovichi, Giorgio Arrigoni, Oriano Marin, and et al. 2024. "By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models" Antioxidants 13, no. 10: 1221. https://doi.org/10.3390/antiox13101221

APA Style

Scalcon, V., Fiorese, F., Albanesi, M., Folda, A., Betti, G., Bellamio, M., Feller, E., Lodovichi, C., Arrigoni, G., Marin, O., & Rigobello, M. P. (2024). By-Products Valorization: Peptide Fractions from Milk Permeate Exert Antioxidant Activity in Cellular and In Vivo Models. Antioxidants, 13(10), 1221. https://doi.org/10.3390/antiox13101221

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