Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals and Agents
2.2. Animals
2.3. Animal Study Design
2.4. Blood Sample Collection
2.5. Measurement of Piglet Weight after Birth and Placental Tissue Collection
2.6. Melatonin Detection
2.7. Detection of Hormone and Biochemical Indicators
2.8. Real-Time Quantitative PCR
2.9. Western Blotting
2.10. Immunofluorescence Detection of AANAT, MTNR1A and MTNR1B
2.11. RNA-Seq for Placenta Tissue
2.12. Statistical Analysis
3. Results
3.1. Effects of Melatonin Supplementation on Levels of Melatonin in Blood and Colostrum
3.2. Effects of Melatonin Supplementation on Reproductive Performance of Sows
3.3. Effects of Melatonin Supplementation on Blood Reproductive Hormone and Other Biochemical Parameters
3.4. Effects of Melatonin Supplementation on Melatonin Synthetic Enzyme AANAT and Melatonin Receptor Expression in Placental Tissue
3.5. Effects of Melatonin Supplementation on the Transcriptome of Placental Tissue of Sows
3.6. Placental Tissue Antioxidant Capacity Test
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Wang, L.; Yan, L.; Han, Q.; Li, G.; Wu, H.; Ma, X.; Zhao, M.; Ma, W.; Ji, P.; Zhang, R.; et al. Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity. Antioxidants 2023, 12, 688. https://doi.org/10.3390/antiox12030688
Wang L, Yan L, Han Q, Li G, Wu H, Ma X, Zhao M, Ma W, Ji P, Zhang R, et al. Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity. Antioxidants. 2023; 12(3):688. https://doi.org/10.3390/antiox12030688
Chicago/Turabian StyleWang, Likai, Laiqing Yan, Qi Han, Guangdong Li, Hao Wu, Xiao Ma, Mengmeng Zhao, Wenkui Ma, Pengyun Ji, Ran Zhang, and et al. 2023. "Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity" Antioxidants 12, no. 3: 688. https://doi.org/10.3390/antiox12030688
APA StyleWang, L., Yan, L., Han, Q., Li, G., Wu, H., Ma, X., Zhao, M., Ma, W., Ji, P., Zhang, R., & Liu, G. (2023). Melatonin Supplementation during the Late Gestational Stage Enhances Reproductive Performance of Sows by Regulating Fluid Shear Stress and Improving Placental Antioxidant Capacity. Antioxidants, 12(3), 688. https://doi.org/10.3390/antiox12030688