Hyperbaric Oxygen Therapy Alleviates Memory and Motor Impairments Following Traumatic Brain Injury via the Modulation of Mitochondrial-Dysfunction-Induced Neuronal Apoptosis in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Study Design
2.3. Controlled Cortical Impact (CCI) Model
2.4. Hyperbaric Oxygen Therapy (HBOT)
2.5. Behavioral Tests
2.5.1. Morris Water Maze
2.5.2. Rotarod Test
2.6. Histology and Microscopy
2.6.1. Tissue Collection
2.6.2. Immunohistochemistry
2.7. Microscopy
2.8. Mitochondrial Function
2.8.1. Mitochondria Isolation from Rat Brain
2.8.2. Mitochondrial Respiration Measurements
2.9. Data Analysis
3. Results
3.1. HBOT Controls the Development of Spatial Learning Impairment after CCI
3.2. HBOT Regulates the Progression of Motor Impairment Following CCI
3.3. HBOT Prevents Neuronal Loss and Glial Cell Proliferation Following CCI
3.4. HBOT Restores Mitochondrial Respiration Following CCI
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
TBI | Traumatic brain injury |
CCI | Controlled cortical impact |
HBOT | Hyperbaric oxygen therapy |
ICP | Intracranial pressure |
ROS | Reactive oxygen species |
TSPO | Translocator protein |
CI | Complex I |
CII | Complex II |
ET | Electron Transfer |
mPTP | Mitochondrial permeability transition pore |
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Sakas, R.; Dan, K.; Edelman, D.; Abu-Ata, S.; Ben-Menashe, A.; Awad-Igbaria, Y.; Francois-Soustiel, J.; Palzur, E. Hyperbaric Oxygen Therapy Alleviates Memory and Motor Impairments Following Traumatic Brain Injury via the Modulation of Mitochondrial-Dysfunction-Induced Neuronal Apoptosis in Rats. Antioxidants 2023, 12, 2034. https://doi.org/10.3390/antiox12122034
Sakas R, Dan K, Edelman D, Abu-Ata S, Ben-Menashe A, Awad-Igbaria Y, Francois-Soustiel J, Palzur E. Hyperbaric Oxygen Therapy Alleviates Memory and Motor Impairments Following Traumatic Brain Injury via the Modulation of Mitochondrial-Dysfunction-Induced Neuronal Apoptosis in Rats. Antioxidants. 2023; 12(12):2034. https://doi.org/10.3390/antiox12122034
Chicago/Turabian StyleSakas, Reem, Katya Dan, Doron Edelman, Saher Abu-Ata, Aviv Ben-Menashe, Yaseen Awad-Igbaria, Jean Francois-Soustiel, and Eilam Palzur. 2023. "Hyperbaric Oxygen Therapy Alleviates Memory and Motor Impairments Following Traumatic Brain Injury via the Modulation of Mitochondrial-Dysfunction-Induced Neuronal Apoptosis in Rats" Antioxidants 12, no. 12: 2034. https://doi.org/10.3390/antiox12122034