NADPH Oxidase-Mediated Testicular Oxidative Imbalance Regulates the TXNIP/NLRP3 Inflammasome Axis Activation after Ischemia Reperfusion Injury
Abstract
:1. Introduction
2. Materials and Methods
2.1. Rat Model of tIRI and Apocynin Treatment
2.2. Histological Analysis
2.3. Western Blot
2.4. Biochemical Assays
2.4.1. Antioxidant Enzymes and Molecules Concentrations
2.4.2. Inflammation Markers Levels
2.4.3. Caspases Activity
2.4.4. NADP/NADPH Levels
2.5. RNA Extraction, Reverse Transcription, and Real-Time PCR
2.6. Statistical Analysis
3. Results
3.1. Antioxidant Effect of NOX Inhibition
3.2. NOX Activates the Expression of the NLRP3 Inflammasome
3.3. NOX-Activated NLRP3 Triggers Germ Cell Inflammatory Response
3.4. NOX Induces Spermatogenic Damage
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Primary Antibody | Dilution | Manufacturer |
---|---|---|
NLRP3 (MBS9127062) | 1:500 | My Bio Source (San Diego, CA, USA) |
GAPDH (MBS9131201) | 1:10,000 | |
ASC (sc-514414) | 1:500 | |
Caspase 1 (sc-56036) | 1:500 | Santa Cruz Biotechnology, Inc. (Dallas, TX, USA) |
GSDMDC1 (sc-393581) | 1:500 | |
MMP-9 (sc-393859) | 1:500 | |
VDUP1 (sc-166234) | 1:500 |
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Almarzouq, D.; Al-Maghrebi, M. NADPH Oxidase-Mediated Testicular Oxidative Imbalance Regulates the TXNIP/NLRP3 Inflammasome Axis Activation after Ischemia Reperfusion Injury. Antioxidants 2023, 12, 145. https://doi.org/10.3390/antiox12010145
Almarzouq D, Al-Maghrebi M. NADPH Oxidase-Mediated Testicular Oxidative Imbalance Regulates the TXNIP/NLRP3 Inflammasome Axis Activation after Ischemia Reperfusion Injury. Antioxidants. 2023; 12(1):145. https://doi.org/10.3390/antiox12010145
Chicago/Turabian StyleAlmarzouq, Duaah, and May Al-Maghrebi. 2023. "NADPH Oxidase-Mediated Testicular Oxidative Imbalance Regulates the TXNIP/NLRP3 Inflammasome Axis Activation after Ischemia Reperfusion Injury" Antioxidants 12, no. 1: 145. https://doi.org/10.3390/antiox12010145