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Article

Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 Cells

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Centre for Inflammation, Centenary Institute and University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW 2007, Australia
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Discipline of Pharmacy, Graduate School of Health, University of Technology Sydney, Sydney, NSW 2007, Australia
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Faculty of Health, Australian Research Centre in Complementary and Integrative Medicine, University of Technology Sydney, Ultimo, NSW 2007, Australia
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School of Pharmaceutical Sciences, Lovely Professional University, Jalandhar-Delhi GT Road, Phagwara 144411, Punjab, India
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School of Pharmacy, Suresh Gyan Vihar University, Jaipur 302017, Rajasthan, India
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Uttaranchal Institute of Pharmaceutical Sciences, Uttaranchal University, Dehradun 248007, Uttarakhand, India
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School of Chemical Engineering, University of New South Wales, Sydney, NSW 2052, Australia
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Australian Centre for NanoMedicine and Centre for Advanced Macromolecular Design, University of New South Wales, Sydney, NSW 2052, Australia
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School of Science, Western Sydney University, Penrith, NSW 2751, Australia
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IDA Business Park, H91 HE94 Galway, Connacht, Ireland
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School of Pharmacy & Biomolecular Sciences, Royal College of Surgeons in Ireland, D02 YN77 Dublin, Leinster, Ireland
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School of Pharmacy & Pharmaceutical Sciences, Trinity College, D02 PN40 Dublin, Leinster, Ireland
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School of Biomedical Engineering, University of Technology Sydney, Sydney, NSW 2007, Australia
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Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, Sydney, NSW 2007, Australia
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Department of Life Sciences, School of Pharmacy, International Medical University, Kuala Lumpur 57000, SGR, Malaysia
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Woolcock Institute of Medical Research, University of Sydney, Sydney, NSW 2037, Australia
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University of Technology Sydney, Faculty of Science, School of Life Sciences, Sydney, NSW 2007, Australia
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Authors to whom correspondence should be addressed.
Academic Editors: Alessandra Napolitano and Stanley Omaye
Antioxidants 2022, 11(5), 873; https://doi.org/10.3390/antiox11050873
Received: 10 March 2022 / Revised: 27 April 2022 / Accepted: 27 April 2022 / Published: 28 April 2022
(This article belongs to the Special Issue Cigarette Smoking: Associated Oxidative Stress and Health Hazards)
Cigarette smoke is considered a primary risk factor for chronic obstructive pulmonary disease. Numerous toxicants present in cigarette smoke are known to induce oxidative stress and airway inflammation that further exacerbate disease progression. Generally, the broncho-epithelial cells and alveolar macrophages exposed to cigarette smoke release massive amounts of oxidative stress and inflammation mediators. Chronic exposure of cigarette smoke leads to premature senescence of airway epithelial cells. This impairs cellular function and ultimately leads to the progression of chronic lung diseases. Therefore, an ideal therapeutic candidate should prevent disease progression by controlling oxidative stress, inflammation, and senescence during the initial stage of damage. In our study, we explored if berberine (an alkaloid)-loaded liquid crystalline nanoparticles (berberine-LCNs)-based treatment to human broncho-epithelial cells and macrophage inhibits oxidative stress, inflammation, and senescence induced by cigarette-smoke extract. The developed berberine-LCNs were found to have favourable physiochemical parameters, such as high entrapment efficiency and sustained in vitro release. The cellular-assay observations revealed that berberine-LCNs showed potent antioxidant activity by suppressing the generation of reactive oxygen species in both broncho-epithelial cells (16HBE) and macrophages (RAW264.7), and modulating the genes involved in inflammation and oxidative stress. Similarly, in 16HBE cells, berberine-LCNs inhibited the cigarette smoke-induced senescence as revealed by X-gal staining, gene expression of CDKN1A (p21), and immunofluorescent staining of p21. Further in-depth mechanistic investigations into antioxidative, anti-inflammatory, and antisenescence research will diversify the current findings of berberine as a promising therapeutic approach for inflammatory lung diseases caused by cigarette smoking. View Full-Text
Keywords: cigarette smoking; airway inflammation; oxidative stress; senescence; berberine; liquid crystalline nanoparticles cigarette smoking; airway inflammation; oxidative stress; senescence; berberine; liquid crystalline nanoparticles
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MDPI and ACS Style

Paudel, K.R.; Panth, N.; Manandhar, B.; Singh, S.K.; Gupta, G.; Wich, P.R.; Nammi, S.; MacLoughlin, R.; Adams, J.; Warkiani, M.E.; Chellappan, D.K.; Oliver, B.G.; Hansbro, P.M.; Dua, K. Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 Cells. Antioxidants 2022, 11, 873. https://doi.org/10.3390/antiox11050873

AMA Style

Paudel KR, Panth N, Manandhar B, Singh SK, Gupta G, Wich PR, Nammi S, MacLoughlin R, Adams J, Warkiani ME, Chellappan DK, Oliver BG, Hansbro PM, Dua K. Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 Cells. Antioxidants. 2022; 11(5):873. https://doi.org/10.3390/antiox11050873

Chicago/Turabian Style

Paudel, Keshav Raj, Nisha Panth, Bikash Manandhar, Sachin Kumar Singh, Gaurav Gupta, Peter R. Wich, Srinivas Nammi, Ronan MacLoughlin, Jon Adams, Majid Ebrahimi Warkiani, Dinesh Kumar Chellappan, Brian G. Oliver, Philip M. Hansbro, and Kamal Dua. 2022. "Attenuation of Cigarette-Smoke-Induced Oxidative Stress, Senescence, and Inflammation by Berberine-Loaded Liquid Crystalline Nanoparticles: In Vitro Study in 16HBE and RAW264.7 Cells" Antioxidants 11, no. 5: 873. https://doi.org/10.3390/antiox11050873

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