Selenium and Redox Enzyme Activity in Pregnant Women Exposed to Methylmercury
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population Recruitment and Sample Collection
2.2. Reagents
2.3. Sample Pre-Treatment for Total Se Analysis
2.4. Selenium Quantification
2.5. Quality Control
2.6. Thioredoxin Reductase and Thioredoxin Activity in Plasma
2.7. Glutathione Peroxidase Activity in Plasma
2.8. Analysis of Total Hg in Blood Samples
2.9. Statistical Analysis
3. Results
3.1. Selenium Concentration in Whole Blood and Plasma
3.2. Redox Proteins Activity
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
Appendix B
Variables Entered/Removed a | |||||||
Model | Variables Entered | Variables Removed | Method | ||||
1 | Hg (µg/L) WB b | Enter | |||||
a. Dependent Variable: Trx (ng/mL) Plasma | |||||||
b. All requested variables entered. | |||||||
Model Summary | |||||||
Model | R | R Square | Adjusted R Square | Std. Error of the Estimate | |||
1 | 0.096 a | 0.009 | 0.007 | 119.71437 | |||
a. Predictors: (Constant), Hg (µg/L) WB | |||||||
ANOVA a | |||||||
Model | Sum of Squares | df | Mean Square | F | Sig. | ||
1 | Regression | 62822.470 | 1 | 62822.470 | 4.384 | 0.037 b | |
Residual | 6721487.713 | 469 | 14331.530 | ||||
Total | 6784310.183 | 470 | |||||
a. Dependent Variable: Trx (ng/mL) Plasma | |||||||
b. Predictors: (Constant), Hg (µg/L) WB | |||||||
Coefficients a | |||||||
Model | Unstandardized Coefficients | Standardized Coefficients | t | Sig. | |||
B | Std. Error | Beta | |||||
1 | (Constant) | 251.551 | 9.196 | 27.353 | 0.000 | ||
Hg (µg/L) WB | −1.733 | 0.828 | −0.096 | −2.094 | 0.037 | ||
a. Dependent Variable: Trx (ng/mL) Plasma |
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Age (years) | 29 ± 6 | |
Gestational weight (Kg) | 67 ± 12 | |
Height (cm) | 165 ± 70 | |
Week of Gestation ** | 20 ± 10 | |
Seafood eating habits (N) | Yes | 428 |
No | 34 | |
DK/DA | 12 |
Gender (N) | Selenium (µg/L) | Location | Reference | ||
---|---|---|---|---|---|
Male | Female | Male | Female | ||
- | 513 167 | - | 65.0 ± 13.1 WB 46.1± 11.1 P | Madeira | Present study |
14 | 13 | 101 ± 1 P | 103 ± 7 P | Not specified | [52] |
136 | 47 | 88 ± 15 P | 81 ± 14 P | Lisbon (urban) | [53] |
48 | 50 | 98 ± 16 P | 81 ± 14 P | Azores, Ponta Delgada (urban) | [54] |
26 | 49 | 110 ± 25 P | 90 ± 21 P | Azores, Rabo de Peixe (fishing) | |
28 | 44 | 84 ± 22 P | 78 ± 18 P | Azores, Rabo de Peixe (rural) | |
28 | 71 | 90 ± 20 P | 82 ± 17 P | Azores, Água Retorta (rural) | |
26 | 63 | 108 ± 16 P | 96 ± 23 P | Ribeira Quente (fishing) | |
62 | 39 | 99.0 ± 20.5 P | 94.4 ± 16.7 P | Lisbon (urban) | [55] |
48 | 50 | 97.9 ± 16.2 P | 88.1 ± 14.5 P | Azores (urban) | |
16 | 19 | 84.5 ± 16.5 P | 84.2 ± 14.4 P | Salvaterra de Magos (rural) |
Women (N) | Selenium (µg/L) | Reference | ||||||
---|---|---|---|---|---|---|---|---|
Trimester | Not Pregnant | Trimester | Not Pregnant | |||||
1 | 2 | 3 | 1 | 2 | 3 | |||
171 * | 81 * | 84 * | - | 65.0 ± 11.2 * | 65.3 ± 13.1 * | 63.0 ± 9.1 * | - | This study |
48 | 48 | 50 | 74 | 64.2 ± 16.9 P | 66.6 ± 17.1 P | 61.6 ± 15.0 P | 70.7 | [60] |
34 | 44 | 52 | 30 | 107 ± 16.0 P | 83.0 ± 17.4 P | 79.8 ± 19.0 P | 109 ± 14.2 P | [61] |
49 | 38 | 30 | 22 | 75.8 ± 16.7 WB | 65.6 ± 14.0 WB | 55.2 ± 12.5 WB | 95 ± 9.3 | [62] |
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Branco, V.; Carvalho, L.; Barboza, C.; Mendes, E.; Cavaco, A.; Carvalho, C. Selenium and Redox Enzyme Activity in Pregnant Women Exposed to Methylmercury. Antioxidants 2022, 11, 2291. https://doi.org/10.3390/antiox11112291
Branco V, Carvalho L, Barboza C, Mendes E, Cavaco A, Carvalho C. Selenium and Redox Enzyme Activity in Pregnant Women Exposed to Methylmercury. Antioxidants. 2022; 11(11):2291. https://doi.org/10.3390/antiox11112291
Chicago/Turabian StyleBranco, Vasco, Luís Carvalho, Cássia Barboza, Eduarda Mendes, Afonso Cavaco, and Cristina Carvalho. 2022. "Selenium and Redox Enzyme Activity in Pregnant Women Exposed to Methylmercury" Antioxidants 11, no. 11: 2291. https://doi.org/10.3390/antiox11112291