Identification and Expression Profiling of Two Saudi Arabia Catalase Genes from Wheat and Barley in Response to Abiotic and Hormonal Stresses
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material
2.2. Isolation and Molecular Cloning of TtCAT1 and HvCAT1 cDNA
2.3. Bioinformatic Analysis of the Sequence of Catalase 1 Genes
2.4. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
2.5. CAT Activity Assay
- AS0 means the absorbance of the blank control;
- and AS2 stand for the absorbance of the samples;
- VT means the total volume of the crude enzyme solution (mL);
- represents the volume of the detected crude enzyme solution (mL);
- FW means the fresh weight of the sample (g), and t means the time from adding H2O2 to the last time (min).
- The activity of the catalase was calculated by the activity level of inoculation minus the level of the control at each corresponding time point.
3. Results
3.1. Isolation and Sequence Analysis of the TtCAT1 and HvCAT1 Genes
3.2. Structural Homology of TtCAT1 and HvCAT1 Proteins
3.3. Interaction Network of Catalase Proteins
3.4. RT-qPCR Analysis of TtCAT1 and HvCAT1 Gene Expressions
3.5. Changes in the TtCAT and HvCAT Activities under Different Abiotic Stresses
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Protein | Total Number of Negatively Charged Residues (Asp + Glu): | Total Number of Positively Charged Residues (Arg + Lys): | Grand Average of Hydropathicity (GRAVY): | MW | Number of aa |
---|---|---|---|---|---|
TtCAT1 | 63 | 58 | −0.595 | 56807.99 | 492 |
HvCAT1 | 63 | 58 | −0.591 | 56567.65 | 492 |
TdCat1 | 65 | 58 | −0.600 | 56768.91 | 492 |
TaCat1 | 63 | 58 | −0.595 | 56807.99 | 492 |
AetCAT1 | 63 | 58 | −0.598 | 56723.87 | 492 |
TmCAT1 | 62 | 58 | −0.590 | 56661.85 | 492 |
BdCat1 | 61 | 58 | −0.608 | 56746.93 | 492 |
AtCat1 | 62 | 58 | −0.574 | 56917.27 | 492 |
TdcCAT1 | 63 | 58 | −0.599 | 56767.93 | 492 |
PgCAT1 | 61 | 57 | −0.575 | 56766.98 | 492 |
SaCAT1 | 61 | 59 | −0.548 | 56921.49 | 492 |
GhCAT1 | 61 | 56 | −0.537 | 56855.20 | 492 |
LjCAT1 | 61 | 57 | −0.547 | 56893.19 | 492 |
EsCAT1 | 59 | 58 | −0.522 | 56689.18 | 492 |
GrCAT1 | 61 | 57 | −0.538 | 56817.20 | 492 |
AiCAT1 | 61 | 58 | −0.562 | 56955.22 | 492 |
Protein | α-Helices | β-Turns | Random Coils | Extended Strands |
---|---|---|---|---|
TtCAT1 | 140 | 27 | 242 | 83 |
HvCAT1 | 136 | 26 | 258 | 72 |
TdCat1 | 135 | 27 | 256 | 74 |
TaCat1 | 140 | 27 | 242 | 83 |
AetCAT1 | 127 | 30 | 258 | 77 |
TmCAT1 | 137 | 30 | 249 | 76 |
BdCat1 | 134 | 29 | 257 | 72 |
AtCat1 | 126 | 31 | 260 | 75 |
TdcCAT1 | 131 | 28 | 258 | 75 |
PgCAT1 | 138 | 29 | 250 | 75 |
SaCAT1 | 135 | 27 | 253 | 77 |
GhCAT1 | 139 | 29 | 249 | 75 |
LjCAT1 | 130 | 25 | 262 | 75 |
EsCAT1 | 137 | 28 | 255 | 72 |
GrCAT1 | 141 | 29 | 248 | 74 |
AiCAT1 | 134 | 32 | 247 | 79 |
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Ghorbel, M.; Besbes, M.; Haddaji, N.; Bouali, N.; Brini, F. Identification and Expression Profiling of Two Saudi Arabia Catalase Genes from Wheat and Barley in Response to Abiotic and Hormonal Stresses. Antioxidants 2022, 11, 2208. https://doi.org/10.3390/antiox11112208
Ghorbel M, Besbes M, Haddaji N, Bouali N, Brini F. Identification and Expression Profiling of Two Saudi Arabia Catalase Genes from Wheat and Barley in Response to Abiotic and Hormonal Stresses. Antioxidants. 2022; 11(11):2208. https://doi.org/10.3390/antiox11112208
Chicago/Turabian StyleGhorbel, Mouna, Malek Besbes, Najla Haddaji, Nouha Bouali, and Faiçal Brini. 2022. "Identification and Expression Profiling of Two Saudi Arabia Catalase Genes from Wheat and Barley in Response to Abiotic and Hormonal Stresses" Antioxidants 11, no. 11: 2208. https://doi.org/10.3390/antiox11112208