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Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol

1
Institute of Human Genetics, Polish Academy of Sciences, 60-479 Poznan, Poland
2
Department of Orthopedics and Traumatology, W. Dega University Hospital, Poznan University of Medical Sciences, 61-545 Poznan, Poland
3
Radiobiology Lab, The Greater Poland Cancer Centre, 61-866 Poznan, Poland
4
Department of Clinical Chemistry and Molecular Diagnostics, Poznan University of Medical Sciences, 61-701 Poznan, Poland
*
Author to whom correspondence should be addressed.
Academic Editor: Stefania Fulle
Antioxidants 2021, 10(6), 959; https://doi.org/10.3390/antiox10060959
Received: 22 April 2021 / Revised: 2 June 2021 / Accepted: 8 June 2021 / Published: 15 June 2021
The aim of the study was to modify human skeletal muscle-derived stem/progenitor cells (SkMDS/PCs) and demonstrate the optimal cell preparation protocol for application in post-infarction hearts. We used conditioned SkMDS/PC culture medium with α-phenyl-N-tert-butyl nitrone (PBN). SkMDS/PCs were cultured under hypoxic conditions and the results were compared to the standard ones. We observed a significant increase of CD-56 positive phenotypic marker the ability to form functional myotubes, increase in the proportion of young cells in cell primary suspensions, and a decrease in the percentage of apoptotic cells among PBN-conditioned cells in normoxia an hypoxia. We also observed significantly higher levels of SOD3 expression; maintained expression of SOD1, SOD2, and CAT; a higher level of BCL2 gene expression; and a rather significant decrease in Hsp70 gene expression in PBN-conditioned SkMDS/PCs compared to the WT population under hypoxic conditions. In addition, significant increase of myogenic genes expression was observed after PBN addition to culture medium, compared to WT population under hypoxia. Interestingly, PBN addition significantly increased the lengths of telomeres under hypoxia. Based on the data obtained, we can postulate that PBN conditioning of human SkMDS/PCs could be a promising step in improving myogenic cell preparation protocol for pro-regenerative treatment of post-infarction hearts. View Full-Text
Keywords: α-phenyl-N-tert-butyl nitrone; heart failure; skeletal muscle derived stem cells; apoptosis; oxidative stress; post-infarction heart α-phenyl-N-tert-butyl nitrone; heart failure; skeletal muscle derived stem cells; apoptosis; oxidative stress; post-infarction heart
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MDPI and ACS Style

Nowaczyk, M.; Malcher, A.; Zimna, A.; Łabędź, W.; Kubaszewski, Ł.; Barczak, W.; Rubiś, B.; Rozwadowska, N.; Kurpisz, M. Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol. Antioxidants 2021, 10, 959. https://doi.org/10.3390/antiox10060959

AMA Style

Nowaczyk M, Malcher A, Zimna A, Łabędź W, Kubaszewski Ł, Barczak W, Rubiś B, Rozwadowska N, Kurpisz M. Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol. Antioxidants. 2021; 10(6):959. https://doi.org/10.3390/antiox10060959

Chicago/Turabian Style

Nowaczyk, Magdalena, Agnieszka Malcher, Agnieszka Zimna, Wojciech Łabędź, Łukasz Kubaszewski, Wojciech Barczak, Błażej Rubiś, Natalia Rozwadowska, and Maciej Kurpisz. 2021. "Addition of Popular Exogenous Antioxidant Agent, PBN, to Culture Media May Be an Important Step to Optimization of Myogenic Stem/Progenitor Cell Preparation Protocol" Antioxidants 10, no. 6: 959. https://doi.org/10.3390/antiox10060959

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