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Article

Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells

1
Department of Agricultural, Food and Nutritional Science, 4-10 Ag/For Building, University of Alberta, Edmonton, AB T6G 2P5, Canada
2
Cardiovascular Research Centre, University of Alberta, Edmonton, AB T6G 2R7, Canada
3
Department of Pharmacology, 7-55 Medical Sciences Building, University of Alberta, Edmonton, AB T6G 2H7, Canada
*
Author to whom correspondence should be addressed.
Academic Editors: Soichiro Nakamura, Shigeru Katayama and Henry M. Corpuz
Antioxidants 2021, 10(2), 290; https://doi.org/10.3390/antiox10020290
Received: 2 January 2021 / Revised: 8 February 2021 / Accepted: 10 February 2021 / Published: 15 February 2021
(This article belongs to the Special Issue Current Trends in Food-Derived Peptidic Antioxidants)
Spent hens are egg-laying hens reaching the end of their egg-laying cycles, being a major byproduct of the egg industry. Recent studies have been focusing on finding new value-added uses for spent hens. We have previously identified four bioactive peptides from spent hen muscle proteins, including three angiotensin-converting enzyme (ACE) inhibitory (ACEi) peptides (VRP, LKY, and VRY), and one ACE2 upregulating (ACE2u) peptide (VVHPKESF (V-F)). In the current study, we further assessed their antioxidant and cytoprotective activities in two vascular cell lines—vascular smooth muscle A7r5 cells (VSMCs) and endothelial EA.hy926 cells (ECs)—upon stimulation by tumor necrosis factor alpha (TNFα) and angiotensin (Ang) II, respectively. The results from our study revealed that all four peptides attenuated oxidative stress in both cells. None of the investigated peptides altered the expression of TNFα receptors in ECs; however, VRY and V-F downregulated Ang II type 1 receptor (AT1R), while V-F upregulated the Mas receptor (MasR) in VSMCs. Further, we found that the antioxidant effects of VRP, LKY, and VRY were likely through acting as direct radical scavengers, while that of V-F was at least partially ascribed to increased endogenous antioxidant enzymes (GPx4 and SOD2) in both cells. Besides, as an ACE2u peptide, V-F exerted antioxidant effect in a MasR-dependent manner, indicating a possible involvement of the upregulated ACE2-MasR axis underlying its antioxidant action. The antioxidant activities of VRP, LKY, VRY, and V-F in vascular cells indicated their multifunctional properties, in addition to their ACEi or ACE2u activity, which supports their potential use as functional food ingredients against hypertension. View Full-Text
Keywords: chicken; laying hen; muscle protein; ACE; ACE2; oxidative stress; antioxidant peptides; antioxidant enzymes chicken; laying hen; muscle protein; ACE; ACE2; oxidative stress; antioxidant peptides; antioxidant enzymes
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MDPI and ACS Style

Fan, H.; Bhullar, K.S.; Wu, J. Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells. Antioxidants 2021, 10, 290. https://doi.org/10.3390/antiox10020290

AMA Style

Fan H, Bhullar KS, Wu J. Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells. Antioxidants. 2021; 10(2):290. https://doi.org/10.3390/antiox10020290

Chicago/Turabian Style

Fan, Hongbing, Khushwant S. Bhullar, and Jianping Wu. 2021. "Spent Hen Muscle Protein-Derived RAS Regulating Peptides Show Antioxidant Activity in Vascular Cells" Antioxidants 10, no. 2: 290. https://doi.org/10.3390/antiox10020290

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