Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro
Abstract
:1. Introduction
2. Materials and Methods
2.1. Chemicals
2.2. Sample Preparation
2.3. MG–BSA Glycation Model
2.3.1. Inhibition Effects on the Formation of Amadori Products
2.3.2. MG-Trapping
2.3.3. Inhibition Effects on the Formation of Carbonyl Groups
2.3.4. Inhibition Effects on the Decrease in ε-NH2 Group
2.3.5. Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis
2.3.6. Fluorescent AGE Analysis
2.4. Cell Culture
2.5. Cell Viability
2.6. MG-Induced Cell Oxidative Damage
2.6.1. Determination of Protective Ability of PNPs against MG-Induced Cell Damage
2.6.2. MG-Induced Cell Growth Rate Assay
2.6.3. ROS Generation
2.7. Statistical Analysis
3. Results
3.1. Inhibition Effects of PNPs on the Formation of Amadori Products
3.2. MG-Trapping Capacity of PNPs
3.3. Inhibition Effects of PNPs on the Formation of the AGEs
3.4. Inhibition Effects on the Formation of the Carbonyl Group and Decrease in the ε-NH2 Group
3.5. Effect of PNPs on Hs68 Cell Viability
3.6. Effects of PNPs on the Viability of MG-Induced Hs68 Cells
3.7. Effect of PNPs on MG-Induced ROS Production in Hs68 Cells
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lin, H.; Lin, T.-Y.; Lin, J.-A.; Cheng, K.-C.; Santoso, S.P.; Chou, C.-H.; Hsieh, C.-W. Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro. Antioxidants 2021, 10, 1589. https://doi.org/10.3390/antiox10101589
Lin H, Lin T-Y, Lin J-A, Cheng K-C, Santoso SP, Chou C-H, Hsieh C-W. Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro. Antioxidants. 2021; 10(10):1589. https://doi.org/10.3390/antiox10101589
Chicago/Turabian StyleLin, His, Ting-Yun Lin, Jer-An Lin, Kuan-Chen Cheng, Shella Permatasari Santoso, Chun-Hsu Chou, and Chang-Wei Hsieh. 2021. "Effect of Pholiota nameko Polysaccharides Inhibiting Methylglyoxal-Induced Glycation Damage In Vitro" Antioxidants 10, no. 10: 1589. https://doi.org/10.3390/antiox10101589