Oleuropein-Rich Leaf Extract as a Broad Inhibitor of Tumour and Macrophage iNOS in an Apc Mutant Rat Model
Abstract
:1. Introduction
2. Materials and Methods
2.1. Olive Leaf Extract’s Preparation and Toxicity
2.2. Cell Lines and Culture Conditions
2.3. MTT Assay
2.4. Animal Maintenance and Ex-Vivo Analysis
2.5. Peritoneal Macrophages Isolation
2.6. Mucosal Samples Collection and RT-PCR Analysis
2.7. Determination of Apoptosis
2.8. Nitric Oxide Assay
2.9. Cytofluorimetric Assay of iNOS in HCT116 Cells
2.10. Western Blotting Analysis
2.11. RNA Extraction and Quantitative PCR
2.12. Statistical Analysis
3. Results
3.1. In Vivo Effect of ORLE on PIRC Rats
3.2. Effect of ORLE on Primed Murine RAW264.7 Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene Name (Accession nr.) | Forward Sequence (5′-3′) | Reverse Sequence (5′-3′) |
---|---|---|
IL-1β (NM_008361) | CCT GCA GCT GGA GAG TGT GGA | CCC ATC AGA GGC AAG GAG GAA |
IL-6 (NM_031168) | CTT CCA TCC AGT TGC CTT CT | TGC ATC ATC GTT GTT CAT AC |
TGF-β (NM_011577.2) | GGC TTC TAG TGC TGA CG | GGG TGC TGT TGT ACA AAG |
18S (NR_003278) | CGC CGC TAG AGG TGA AAT TCT | CGA ACC TCC GAC TTT CGT TCT |
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Ruzzolini, J.; Chioccioli, S.; Monaco, N.; Peppicelli, S.; Andreucci, E.; Urciuoli, S.; Romani, A.; Luceri, C.; Tortora, K.; Calorini, L.; et al. Oleuropein-Rich Leaf Extract as a Broad Inhibitor of Tumour and Macrophage iNOS in an Apc Mutant Rat Model. Antioxidants 2021, 10, 1577. https://doi.org/10.3390/antiox10101577
Ruzzolini J, Chioccioli S, Monaco N, Peppicelli S, Andreucci E, Urciuoli S, Romani A, Luceri C, Tortora K, Calorini L, et al. Oleuropein-Rich Leaf Extract as a Broad Inhibitor of Tumour and Macrophage iNOS in an Apc Mutant Rat Model. Antioxidants. 2021; 10(10):1577. https://doi.org/10.3390/antiox10101577
Chicago/Turabian StyleRuzzolini, Jessica, Sofia Chioccioli, Noemi Monaco, Silvia Peppicelli, Elena Andreucci, Silvia Urciuoli, Annalisa Romani, Cristina Luceri, Katia Tortora, Lido Calorini, and et al. 2021. "Oleuropein-Rich Leaf Extract as a Broad Inhibitor of Tumour and Macrophage iNOS in an Apc Mutant Rat Model" Antioxidants 10, no. 10: 1577. https://doi.org/10.3390/antiox10101577