Next Article in Journal
Data Integration for Microarrays: Enhanced Inference for Gene Regulatory Networks
Previous Article in Journal
Label and Label-Free Detection Techniques for Protein Microarrays
Previous Article in Special Issue
Tissue Microarray Technology for Molecular Applications: Investigation of Cross-Contamination between Tissue Samples Obtained from the Same Punching Device
Please note that, as of 18 July 2017, Microarrays has been renamed to High-Throughput and is now published here.

Re-Punching Tissue Microarrays Is Possible: Why Can This Be Useful and How to Do It

Molecular Pathology Division, Institute of Pathology, University Hospital of Basel, CH-4031 Basel, Switzerland
Author to whom correspondence should be addressed.
Academic Editor: Luigi Terracciano
Microarrays 2015, 4(2), 245-254;
Received: 15 February 2015 / Revised: 25 April 2015 / Accepted: 29 April 2015 / Published: 11 May 2015
Tissue microarray (TMA) methodology allows the concomitant analysis of hundreds of tissue specimens arrayed in the same manner on a recipient block. Subsequently, all samples can be processed under identical conditions, such as antigen retrieval procedure, reagent concentrations, incubation times with antibodies/probes, and escaping the inter-assays variability. Therefore, the use of TMA has revolutionized histopathology translational research projects and has become a tool very often used for putative biomarker investigations. TMAs are particularly relevant for large scale analysis of a defined disease entity. In the course of these exploratory studies, rare subpopulations can be discovered or identified. This can refer to subsets of patients with more particular phenotypic or genotypic disease with low incidence or to patients receiving a particular treatment. Such rare cohorts should be collected for more specific investigations at a later time, when, possibly, more samples of a rare identity will be available as well as more knowledge derived from concomitant, e.g., genetic, investigations will have been acquired. In this article we analyze for the first time the limits and opportunities to construct new TMA blocks using tissues from older available arrays and supplementary donor blocks. In summary, we describe the reasons and technical details for the construction of rare disease entities arrays. View Full-Text
Show Figures

Figure 1

MDPI and ACS Style

Lacombe, A.; Carafa, V.; Schneider, S.; Sticker-Jantscheff, M.; Tornillo, L.; Eppenberger-Castori, S. Re-Punching Tissue Microarrays Is Possible: Why Can This Be Useful and How to Do It. Microarrays 2015, 4, 245-254.

AMA Style

Lacombe A, Carafa V, Schneider S, Sticker-Jantscheff M, Tornillo L, Eppenberger-Castori S. Re-Punching Tissue Microarrays Is Possible: Why Can This Be Useful and How to Do It. Microarrays. 2015; 4(2):245-254.

Chicago/Turabian Style

Lacombe, Aurélien, Vincenza Carafa, Sandra Schneider, Melanie Sticker-Jantscheff, Luigi Tornillo, and Serenella Eppenberger-Castori. 2015. "Re-Punching Tissue Microarrays Is Possible: Why Can This Be Useful and How to Do It" Microarrays 4, no. 2: 245-254.

Find Other Styles

Article Access Map by Country/Region

Only visits after 24 November 2015 are recorded.
Back to TopTop