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3D Cell Culture in Alginate Hydrogels

FMC BioPolymer AS, Industriveien 33, 1337 Sandvika, Norway
Author to whom correspondence should be addressed.
Academic Editors: Mohammad Reza Lornejad-Schäfer and Christine Schäfer
Microarrays 2015, 4(2), 133-161;
Received: 31 January 2015 / Revised: 16 March 2015 / Accepted: 17 March 2015 / Published: 24 March 2015
(This article belongs to the Special Issue Advantages of Three Dimensional (3D) Cell Cultures)
This review compiles information regarding the use of alginate, and in particular alginate hydrogels, in culturing cells in 3D. Knowledge of alginate chemical structure and functionality are shown to be important parameters in design of alginate-based matrices for cell culture. Gel elasticity as well as hydrogel stability can be impacted by the type of alginate used, its concentration, the choice of gelation technique (ionic or covalent), and divalent cation chosen as the gel inducing ion. The use of peptide-coupled alginate can control cell–matrix interactions. Gelation of alginate with concomitant immobilization of cells can take various forms. Droplets or beads have been utilized since the 1980s for immobilizing cells. Newer matrices such as macroporous scaffolds are now entering the 3D cell culture product market. Finally, delayed gelling, injectable, alginate systems show utility in the translation of in vitro cell culture to in vivo tissue engineering applications. Alginate has a history and a future in 3D cell culture. Historically, cells were encapsulated in alginate droplets cross-linked with calcium for the development of artificial organs. Now, several commercial products based on alginate are being used as 3D cell culture systems that also demonstrate the possibility of replacing or regenerating tissue. View Full-Text
Keywords: alginate; hydrogel; 3D; drug development; tissue regeneration; drug discovery; AlgiMatrix®; NovaMatrix®-3D; beads; bioprinting alginate; hydrogel; 3D; drug development; tissue regeneration; drug discovery; AlgiMatrix®; NovaMatrix®-3D; beads; bioprinting
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MDPI and ACS Style

Andersen, T.; Auk-Emblem, P.; Dornish, M. 3D Cell Culture in Alginate Hydrogels. Microarrays 2015, 4, 133-161.

AMA Style

Andersen T, Auk-Emblem P, Dornish M. 3D Cell Culture in Alginate Hydrogels. Microarrays. 2015; 4(2):133-161.

Chicago/Turabian Style

Andersen, Therese, Pia Auk-Emblem, and Michael Dornish. 2015. "3D Cell Culture in Alginate Hydrogels" Microarrays 4, no. 2: 133-161.

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