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Volume 12
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10.3390/brainsci12020282
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Article

Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma

by
Shahad Iqneibi
1,
Jamil Nazzal
1,
Basma Owda
1,
Hala Sultan
1,
Runa Amoudi
1,
Justin Z. Amarin
2,
Sura Al-Ghnimat
2,
Mamoun Ahram
3,† and
Maysa Al-Hussaini
4,*,†
1
School of Medicine, The University of Jordan, Amman 11942, Jordan
2
Office of Scientific Affairs and Research, King Hussein Cancer Center, Amman 11941, Jordan
3
Department of Physiology and Biochemistry, School of Medicine, The University of Jordan, Amman 11942, Jordan
4
Department of Pathology and Laboratory Medicine, King Hussein Cancer Center, Amman 11941, Jordan
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Brain Sci. 2022, 12(2), 282; https://doi.org/10.3390/brainsci12020282
Submission received: 30 December 2021 / Revised: 5 February 2022 / Accepted: 8 February 2022 / Published: 17 February 2022
(This article belongs to the Section Neuro-oncology)
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Abstract

p27 and p57 are tumor suppressors that are dysregulated in many cancers. We investigated the immunohistochemical expression of p27 and p57 in ependymoma, with a secondary emphasis on cyclin D1, nestin, and Ki-67. Sixty-five patients diagnosed with ependymoma were included. Clinical and tumoral data were retrieved, and the expression of p27, p57, cyclin D1, nestin, and Ki-67 was measured. Pearson’s χ2 test was used to measure associations and the Kaplan–Meier method was used for survival analysis. p27 underexpression was significantly associated with pseudopalisading necrosis in tumors with foci of necrosis (p = 0.004). Cyclin D1 overexpression was associated with intracranial (p = 0.044), recurrent (p = 0.022) and grade 3 tumors (p = 0.016); nestin overexpression was associated with supratentorial (p = 0.025), mitotically active (p < 0.001), and grade 3 tumors (p = 0.004); Ki-67 overexpression was associated with supratentorial (p = 0.044) and grade 3 tumors (p < 0.001) and the 3 main features of anaplasia. None of the markers were intercorrelated or predictive of overall survival. In conclusion, p27 underexpression in tumors with foci of necrosis signals a pseudopalisading pattern. Cyclin D1, nestin, and Ki-67 are useful markers in ependymoma, but evidence-based cutoff values are required to standardize this interpretation.
Keywords: cyclin D1; cyclin-dependent kinase inhibitor p27; cyclin-dependent kinase inhibitor p57; ependymoma; Ki-67 antigen; nestin cyclin D1; cyclin-dependent kinase inhibitor p27; cyclin-dependent kinase inhibitor p57; ependymoma; Ki-67 antigen; nestin

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MDPI and ACS Style

Iqneibi, S.; Nazzal, J.; Owda, B.; Sultan, H.; Amoudi, R.; Amarin, J.Z.; Al-Ghnimat, S.; Ahram, M.; Al-Hussaini, M. Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma. Brain Sci. 2022, 12, 282. https://doi.org/10.3390/brainsci12020282

AMA Style

Iqneibi S, Nazzal J, Owda B, Sultan H, Amoudi R, Amarin JZ, Al-Ghnimat S, Ahram M, Al-Hussaini M. Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma. Brain Sciences. 2022; 12(2):282. https://doi.org/10.3390/brainsci12020282

Chicago/Turabian Style

Iqneibi, Shahad, Jamil Nazzal, Basma Owda, Hala Sultan, Runa Amoudi, Justin Z. Amarin, Sura Al-Ghnimat, Mamoun Ahram, and Maysa Al-Hussaini. 2022. "Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma" Brain Sciences 12, no. 2: 282. https://doi.org/10.3390/brainsci12020282

APA Style

Iqneibi, S., Nazzal, J., Owda, B., Sultan, H., Amoudi, R., Amarin, J. Z., Al-Ghnimat, S., Ahram, M., & Al-Hussaini, M. (2022). Immunohistochemical Expression of p27Kip1, p57Kip2, Cyclin D1, Nestin, and Ki-67 in Ependymoma. Brain Sciences, 12(2), 282. https://doi.org/10.3390/brainsci12020282

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