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Open AccessArticle

Persistent Roseoloviruses Infection in Adult Patients with Epilepsy

1
Institute of Microbiology and Virology, Rīga Stradiņš University, LV-1067 Rīga, Latvia
2
Department of Neurology and Neurosurgery, Riga East Clinical University Hospital “Gailezers”, LV-1038 Rīga, Latvia
3
Department of Microbiology, Tumor and Cell Biology (MTC), Karolinska Institutet, 171 65 Solna, Sweden
*
Author to whom correspondence should be addressed.
Brain Sci. 2020, 10(5), 287; https://doi.org/10.3390/brainsci10050287
Received: 22 April 2020 / Revised: 8 May 2020 / Accepted: 9 May 2020 / Published: 11 May 2020
Background: Human herpesviruses (HHV)-6A, HHV-6B and HHV-7 are considered to be involved in the pathogenesis of epilepsy, a common neurological disorder. The objective of this study was to determine the association of roseoloviruses infection with epilepsy. Methods: 53 epilepsy patients and 104 ordinary blood donors were analyzed to determine presence of virus-specific antibodies by enzyme-linked immunosorbent assay (ELISA) and immunofluorescence assay (IFA), genomic sequences, viral load and gene expression by polymerase chain reactions (PCRs) and restriction analysis, HHV-6 protein expression by IFA and level of cytokines by ELISA. Results: Roseoloviruses genomic sequences in DNA samples from whole blood were found in 86.8% of patients versus 54.8% of controls and active infection was revealed only in patients with epilepsy (19.6% of roseolovirus-positive patients). Significantly higher viral load and more frequent gene expression was detected in patients compared to the controls. HHV-6-encoded protein expression was demonstrated in 53.3% of patients with previously detected HHV-6 DNA. Changes in level of cytokines were determined in patients with elevated viral load compared to the patients without elevated viral loads and to the controls. Conclusions: Results on frequent active HHV-6 and HHV-7 infection in epilepsy patient’ peripheral blood indicate on possible involvement of these viruses in the disease development.
Keywords: human herpesvirus-6; human herpesvirus-7; reactivation; epilepsy human herpesvirus-6; human herpesvirus-7; reactivation; epilepsy
MDPI and ACS Style

Rasa-Dzelzkaleja, S.; Gravelsina, S.; Chapenko, S.; Krukle, Z.-N.; Svirskis, S.; Suna, N.; Kashuba, E.; Karelis, G.; Murovska, M. Persistent Roseoloviruses Infection in Adult Patients with Epilepsy. Brain Sci. 2020, 10, 287.

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