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Appl. Sci. 2019, 9(3), 577; https://doi.org/10.3390/app9030577

A New “Turn-On” Fluorescence Probe for Al3+ Detection and Application Exploring in Living Cell and Real Samples

1
School of Materials Science and Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
2
School of Chemistry and Pharmaceutical Engineering, Qilu University of Technology, Shandong Academy of Sciences, Jinan 250353, China
*
Author to whom correspondence should be addressed.
Received: 10 January 2019 / Revised: 29 January 2019 / Accepted: 4 February 2019 / Published: 10 February 2019
(This article belongs to the Section Applied Biosciences and Bioengineering)
Full-Text   |   PDF [7888 KB, uploaded 10 February 2019]   |  

Abstract

An excess of Al3+ will lead to biological disorders and even many diseases. Therefore, detecting the levels of Al3+ in the human body has drawn great attention for health monitoring. The fluorescence method has been broadly applied because of high sensitivity, real-time detection, and intracellular imaging. In this work, a new probe with “turn-on” fluorescence based on Schiff base derivative, 3,6-imine-triphenylamine-(9-ethyl) carbazole (ITEC), has been successfully synthesized and studied. The high selectivity and sensitivity of ITEC to Al3+ were verified by fluorescence spectra and the detection limit was 2.19 nmol/L. A 1:2 stoichiometry of ITEC-Al3+ was obtained by the 1H NMR spectra and Job’s plot. Furthermore, ITEC was successfully applied to the detection of Al3+ with different concentrations in living HeLa cells. The analog experiments about nature contamination of Al3+ in cells and real samples were finished. View Full-Text
Keywords: Al3+; Schiff base derivative; fluorescent probe; imaging; real samples Al3+; Schiff base derivative; fluorescent probe; imaging; real samples
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Gao, Z.-Y.; Zhang, C.-J.; Zhang, X.; Xing, S.; Yao, J.-S.; Qiao, C.-D.; Liu, W.-L. A New “Turn-On” Fluorescence Probe for Al3+ Detection and Application Exploring in Living Cell and Real Samples. Appl. Sci. 2019, 9, 577.

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