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Article

Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes

1
Centro de Biotecnología de los Recursos Naturales (CENBIO), Facultad de Ciencias Agrarias y Forestales, Universidad Católica del Maule, Avda. San Miguel 3605, Talca 3480112, Chile
2
Centro de Investigación en Estudios Avanzados del Maule (CIEAM), Vicerrectoría de Investigación y Postgrado, Universidad Católica del Maule, Avda. San Miguel 3605, Talca 3480112, Chile
3
Institute of Experimental Internal Medicine, Otto-von-Guericke University, Magdeburg 39120, Germany
4
Facultad de Ingeniería, Ciencias y Tecnología, Universidad Bernardo O’Higgins, Santiago 8370993, Chile
*
Author to whom correspondence should be addressed.
Appl. Sci. 2019, 9(20), 4419; https://doi.org/10.3390/app9204419
Received: 9 August 2019 / Revised: 12 October 2019 / Accepted: 16 October 2019 / Published: 18 October 2019
(This article belongs to the Special Issue Cellulose Conversion Technology)
The cost of cellulase enzymes is a main contributor to the operational cost of a biorefinery producing ethanol from lignocellulosic material. Therefore, onsite production of enzymes using low-value substrates might be an option to make a bio-based facility more economical, while improving environmental sustainability. Food manufacturing wastes (FMWs), such as olive mill solids, tomato pomace, and grape pomace, are some of the main wastes produced by the food industry in Chile. FMWs are mostly composed of lignocellulosic material, which is primarily made of cellulose. A fungal strain obtained from olive stones was identified as a Trichoderma sp. and characterized by molecular and morphological techniques. This strain was able to grow on three FMWs in both liquid and solid cultures. In liquid cultures, cellulase and β-glucosidase activities from the culture supernatants were quantified. Identification of extracellular proteins using mass spectrometry revealed the presence of endoglucanases, exoglucanases, and β-glucosidases. Cellulase production from agroindustrial residues could be an excellent opportunity to utilize FMWs as well as decrease enzyme production costs in biorefinery processes. View Full-Text
Keywords: enzymes; food manufacturing wastes; olive mill solid waste; tomato pomace; grape pomace; cellulases; Trichoderma sp., bioethanol; biorefinery enzymes; food manufacturing wastes; olive mill solid waste; tomato pomace; grape pomace; cellulases; Trichoderma sp., bioethanol; biorefinery
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MDPI and ACS Style

Gordillo-Fuenzalida, F.; Echeverria-Vega, A.; Cuadros-Orellana, S.; Faundez, C.; Kähne, T.; Morales-Vera, R. Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes. Appl. Sci. 2019, 9, 4419. https://doi.org/10.3390/app9204419

AMA Style

Gordillo-Fuenzalida F, Echeverria-Vega A, Cuadros-Orellana S, Faundez C, Kähne T, Morales-Vera R. Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes. Applied Sciences. 2019; 9(20):4419. https://doi.org/10.3390/app9204419

Chicago/Turabian Style

Gordillo-Fuenzalida, Felipe, Alex Echeverria-Vega, Sara Cuadros-Orellana, Claudia Faundez, Thilo Kähne, and Rodrigo Morales-Vera. 2019. "Cellulases Production by a Trichoderma sp. Using Food Manufacturing Wastes" Applied Sciences 9, no. 20: 4419. https://doi.org/10.3390/app9204419

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