Next Article in Journal
A Modular Cell Balancer Based on Multi-Winding Transformer and Switched-Capacitor Circuits for a Series-Connected Battery String in Electric Vehicles
Previous Article in Journal
Mode Profile Shaping in Wire Media: Towards An Experimental Verification
Article Menu
Issue 8 (August) cover image

Export Article

Open AccessArticle
Appl. Sci. 2018, 8(8), 1277;

Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation

Department of Civil Engineering, Hongik University, Seoul 04066, Korea
Civil Design Team, Daelim Industrial Corp. Ltd., Seoul 08826, Korea
Author to whom correspondence should be addressed.
Received: 24 May 2018 / Revised: 28 July 2018 / Accepted: 30 July 2018 / Published: 1 August 2018
(This article belongs to the Section Applied Biosciences and Bioengineering)
Full-Text   |   PDF [1265 KB, uploaded 2 August 2018]   |  


In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii. View Full-Text
Keywords: MICP; optimal condition; Staphylococcus saprophyticus; Sporosarcina pasteurii MICP; optimal condition; Staphylococcus saprophyticus; Sporosarcina pasteurii

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Kim, G.; Kim, J.; Youn, H. Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation. Appl. Sci. 2018, 8, 1277.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top