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Appl. Sci. 2018, 8(8), 1277; https://doi.org/10.3390/app8081277

Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation

1
Department of Civil Engineering, Hongik University, Seoul 04066, Korea
2
Civil Design Team, Daelim Industrial Corp. Ltd., Seoul 08826, Korea
*
Author to whom correspondence should be addressed.
Received: 24 May 2018 / Revised: 28 July 2018 / Accepted: 30 July 2018 / Published: 1 August 2018
(This article belongs to the Section Applied Biosciences and Bioengineering)
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Abstract

In this study, the amount of calcite precipitate resulting from microbially induced calcite precipitation (MICP) was estimated in order to determine the optimal conditions for precipitation. Two microbial species (Staphylococcus saprophyticus and Sporosarcina pasteurii) were tested by varying certain parameters such as (1) initial potential of hydrogen (pH) of urea-CaCl2 medium, (2) temperature during precipitation, and (3) the reaction duration. The pH values used for testing were 6, 7, 8, 9, and 10, the temperatures were 20, 30, 40, and 50 °C, and the reaction durations were 2, 3, and 4 days. Maximum calcite precipitation was observed at a pH of 7 and temperature of 30 °C. Most of the precipitation occurred within a reaction duration of 3 days. Under similar conditions, the amount of calcite precipitated by S. saprophyticus was estimated to be five times more than that by S. pasteurii. Both the species were sensitive to temperature; however, S. saprophyticus was less sensitive to pH and required a shorter reaction duration than S. pasteurii. View Full-Text
Keywords: MICP; optimal condition; Staphylococcus saprophyticus; Sporosarcina pasteurii MICP; optimal condition; Staphylococcus saprophyticus; Sporosarcina pasteurii
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Kim, G.; Kim, J.; Youn, H. Effect of Temperature, pH, and Reaction Duration on Microbially Induced Calcite Precipitation. Appl. Sci. 2018, 8, 1277.

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