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Characterization of Human Dermal Papilla Cells in Alginate Spheres

1,2,†, 4,†, 1,2, 1,2, 1,2, 5, 2,3,* and 1,2,3,*
1
Department of Anatomy, Kyungpook National University School of Medicine, 680 Gukchaebosang-ro, Daegu 41944, Korea
2
Department of Biomedical Science, Biomedical Research Institute, Kyungpook National University, Daegu 41940, Korea
3
Joint Institute for Regenerative Medicine, Kyungpook National University Hospital, Daegu 41940, Korea
4
Department of Microbiology, Kyungpook National University School of Medicine, 680 Gukchaebosang-ro, Daegu 41944, Korea
5
Department of Developmental and Cell Biology, University of California, Irvine, CA 92697, USA
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Appl. Sci. 2018, 8(10), 1993; https://doi.org/10.3390/app8101993
Received: 20 July 2018 / Revised: 2 October 2018 / Accepted: 3 October 2018 / Published: 19 October 2018
(This article belongs to the Special Issue Synthesis and Application of Microcapsules)
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Abstract

Maintenance of trichogenecity of dermal papilla cells (DPCs) have been a problem during cell therapy for androgenic alopecia, as they lose their regenerative potential in in vitro culture. Various spheroid culture techniques are used to increase and maintain trichogenecity of these cells. However, there are some critical drawbacks in these methods. Applying a hydrocell plate for sphere formation or hanging drop methods by hand would be difficult to control the size and cell density inside it. It would be difficult to commercialize or mass production for clinical therapy. In aim to address and overcome these drawbacks, we have introduced alginate sphere. The alginate sphere of DPCs were prepared by electrospinning at different voltages to control the size of sphere. Then the obtained alginate spheres were evaluated for cellular dynamics and density of DPCs under different conditions. In this study, we found that DPCs do not proliferate in alginate sphere. However, the number of DPCs were maintained and found to be in dormant state. Further, the dormant DPCs in the alginate sphere have upregulated DPC signature genes (SOX2, ALPL, WIF1, Noggin, BMP4 and VCAN) and proliferative capacity. Thus, we speculate that alginate sphere environment maintains the dormancy of DPCs with increased trichogenecity. View Full-Text
Keywords: alginate sphere; dermal papilla cell; trichogenecity; dormancy alginate sphere; dermal papilla cell; trichogenecity; dormancy
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Mali, N.M.; Kim, Y.-H.; Park, J.M.; Kim, D.; Heo, W.; Dao, B.L.; Lim, J.O.; Oh, J.W. Characterization of Human Dermal Papilla Cells in Alginate Spheres. Appl. Sci. 2018, 8, 1993.

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