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Article

The Characteristics of Intrinsic Fluorescence of Type I Collagen Influenced by Collagenase I

1
College of Leather Chemistry and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan 250353, China
2
Room L1.42 Gorlaeus Laboratory, Leiden University, 2311 EZ Leiden, The Netherlands
*
Authors to whom correspondence should be addressed.
Appl. Sci. 2018, 8(10), 1947; https://doi.org/10.3390/app8101947
Received: 28 September 2018 / Revised: 12 October 2018 / Accepted: 13 October 2018 / Published: 16 October 2018
(This article belongs to the Section Chemical and Molecular Sciences)
The triple helix structure of collagen can be degraded by collagenase. In this study, we explored how the intrinsic fluorescence of type I collagen was influenced by collagenase I. We found that tyrosine was the main factor that could successfully excite the collagen fluorescence. Initially, self-assembly behavior of collagen resulted in a large amount of tyrosine wrapped with collagen, which decreased the fluorescence intensity of type I collagen. After collagenase cleavage, some wrapped-tyrosine could be exposed and thereby the intrinsic fluorescence intensity of collagen increased. By observation and analysis, the influence of collagenase to intrinsic fluorescence of collagen was investigated and elaborated. Furthermore, collagenase cleavage to the special triple helix structure of collagen would result in a slight improvement of collagen thermostability, which was explained by the increasing amount of terminal peptides. These results are helpful and effective for reaction mechanism research related to collagen, which can be observed by fluorescent technology. Meantime, the reaction behaviors of both collagenase and collagenolytic proteases can also be analyzed by fluorescent technology. In conclusion, this research provides a foundation for the further investigation of collagen reactions in different areas, such as medicine, nutrition, food and agriculture. View Full-Text
Keywords: collagen; collagenase; intrinsic fluorescence; tyrosine; fluorescence spectroscopy collagen; collagenase; intrinsic fluorescence; tyrosine; fluorescence spectroscopy
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MDPI and ACS Style

Shen, Y.; Zhu, D.; Lu, W.; Liu, B.; Li, Y.; Cao, S. The Characteristics of Intrinsic Fluorescence of Type I Collagen Influenced by Collagenase I. Appl. Sci. 2018, 8, 1947. https://doi.org/10.3390/app8101947

AMA Style

Shen Y, Zhu D, Lu W, Liu B, Li Y, Cao S. The Characteristics of Intrinsic Fluorescence of Type I Collagen Influenced by Collagenase I. Applied Sciences. 2018; 8(10):1947. https://doi.org/10.3390/app8101947

Chicago/Turabian Style

Shen, Yiming, Deyi Zhu, Wenhui Lu, Bing Liu, Yanchun Li, and Shan Cao. 2018. "The Characteristics of Intrinsic Fluorescence of Type I Collagen Influenced by Collagenase I" Applied Sciences 8, no. 10: 1947. https://doi.org/10.3390/app8101947

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