Next Article in Journal
A Real Model of a Micro-Grid to Improve Network Stability
Previous Article in Journal
Modified Chaos Particle Swarm Optimization-Based Optimized Operation Model for Stand-Alone CCHP Microgrid
Project Report

Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal

1
Bionanopark, Laboratory of Industrial Biotechnology Ltd. Dubois 114/116, 93-465 Łódź, Poland
2
Institute of Materials Science and Engineering, Lodz University of Technology, Stefanowskiego 1/15, 90-924 Łódź, Poland
3
Institute of Fermentation Technology and Microbiology, Lodz University of Technology, Wólczańska 171/173, 90-924 Łódź, Poland
4
JHJ Sp. z o.o., 63-308 Gizałki, Poland
*
Author to whom correspondence should be addressed.
Author has passed away.
Appl. Sci. 2017, 7(8), 756; https://doi.org/10.3390/app7080756
Received: 21 June 2017 / Revised: 21 July 2017 / Accepted: 21 July 2017 / Published: 25 July 2017
The aim of this work was to develop the composition of a medium for the cultivation of six microbial strains forming a deodorizing consortium: Pseudomonas fluorescens, Enterococcus faecium, Bacillus subtilis, Bacillus megaterium, Leuconostoc mesenteroides and Lactobacillus plantarum. The study focused on the optimization of a highly efficient culture medium composed of readily available components of plant origin to maximize microbial biomass yields, and to create a less expensive alternative to the commercial Tryptic Soy Broth medium (TSB). After preliminary efficiency screening of all tested media components, we selected four substrates for further optimization—soy protein concentrate (SPC), glucose or sucrose, and phosphate salts. The final concentrations of all components were fine-tuned using the Taguchi design for experiments according to an L9 array. Taguchi optimization led to formulation of a culture medium, which was approximately 5 times cheaper than TSB (depending on the components used). Consequently, microbial biomass yields were improved by up to 15-fold (1564%), depending on the strain. The results obtained in the laboratory experiments were then confirmed in pilot- (42 L) and industrial- (300 L) scale fermentation. Our results show that this method of using a parallel culture microbioreactor with the Taguchi approach can be recommended for optimization of culture media based on substrates of plant origin. View Full-Text
Keywords: medium optimization; Taguchi method; bacterial biomass production; Biolector; components of plant origin medium optimization; Taguchi method; bacterial biomass production; Biolector; components of plant origin
Show Figures

Figure 1

MDPI and ACS Style

Makowski, K.; Matusiak, K.; Borowski, S.; Bielnicki, J.; Tarazewicz, A.; Maroszyńska, M.; Leszczewicz, M.; Powałowski, S.; Gutarowska, B. Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal. Appl. Sci. 2017, 7, 756. https://doi.org/10.3390/app7080756

AMA Style

Makowski K, Matusiak K, Borowski S, Bielnicki J, Tarazewicz A, Maroszyńska M, Leszczewicz M, Powałowski S, Gutarowska B. Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal. Applied Sciences. 2017; 7(8):756. https://doi.org/10.3390/app7080756

Chicago/Turabian Style

Makowski, Krzysztof, Katarzyna Matusiak, Sebastian Borowski, Jakub Bielnicki, Alicja Tarazewicz, Marta Maroszyńska, Martyna Leszczewicz, Szymon Powałowski, and Beata Gutarowska. 2017. "Optimization of a Culture Medium Using the Taguchi Approach for the Production of Microorganisms Active in Odorous Compound Removal" Applied Sciences 7, no. 8: 756. https://doi.org/10.3390/app7080756

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop