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Appl. Sci. 2017, 7(5), 533;

Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants

Technische Thermodynamik and MAPEX Center for Materials and Processes, Universität Bremen, Badgasteiner Straße 1, 28215 Bremen, Germany
School of Engineering, University of Aberdeen, Fraser Noble Building, Aberdeen AB24 3UE, UK
Erlangen Graduate in Advanced Optical Technologies (SAOT), Friedrich-Alexander-Universität Erlangen-Nürnberg, 91054 Erlangen, Germany
School of Chemical Engineering and Analytical Science, The University of Manchester, Manchester M13 9PL, UK
Department of Biological and Agricultural Engineering, Faculty of Engineering, Universiti Putra Malaysia, Serdang 43400, Malaysia
Author to whom correspondence should be addressed.
Academic Editor: Jun Kubota
Received: 23 April 2017 / Revised: 18 May 2017 / Accepted: 19 May 2017 / Published: 22 May 2017
(This article belongs to the Special Issue Optics and Spectroscopy for Fluid Characterization)
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Biosurfactants are produced by microorganisms and represent amphiphilic compounds with polar and non-polar moieties; hence they can be used to stabilize emulsions, e.g., in the cosmetic and food sectors. Their structure and its changes when exposed to light and elevated temperature are yet to be fully understood. In this study, we demonstrate that attenuated total reflection infrared (ATR-IR) spectroscopy is a useful tool for the analysis of biosurfactants, using rhamnolipids produced by fermentation as an example. A key feature is that the analytical method does not require sample preparation despite the high viscosity of the purified natural product. View Full-Text
Keywords: vibrational spectroscopy; FTIR; Pseudomonas aeruginosa; rhamnose; fatty acid vibrational spectroscopy; FTIR; Pseudomonas aeruginosa; rhamnose; fatty acid

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Kiefer, J.; Radzuan, M.N.; Winterburn, J. Infrared Spectroscopy for Studying Structure and Aging Effects in Rhamnolipid Biosurfactants. Appl. Sci. 2017, 7, 533.

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