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Appl. Sci. 2017, 7(3), 195;

Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode

Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Chemical Biology Center, Lishui Institute of Agricultural Sciences, Lishui 323000, Zhejiang, China
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
School of Pharmaceutical Sciences, Wenzhou Medical University, Wenzhou 325035, Zhejiang, China
Authors to whom correspondence should be addressed.
Academic Editor: Chih-Ching Huang
Received: 16 November 2016 / Revised: 25 January 2017 / Accepted: 10 February 2017 / Published: 23 February 2017
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Traditional She medicine is part of China’s cultural heritage and has become remarkably popular worldwide. The Shi-Liang tea is made from the processed leaves of Chimonanthus salicifolius S. Y. Hu and Chimonanthus zhejiangensis M. C. Liu. To ensure the safety and efficacy of Shi-Liang tea, we used DNA barcoding based on the internal transcribed spacer 2 (ITS2) of nuclear ribosomal DNA to distinguish the original plant sources of Shi-Liang tea from closely related species. All 71 ITS2 sequences were aligned by Clustal-W, and genetic distances were computed using MEGA 6.0 according to the Kimura 2-parameter model. The results indicated that the sequence lengths of ITS2 regions of the original plants of Shi-Liang tea and closely related species ranged from 256 bp to 260 bp. Interspecific genetic distances ranged from 0 to 0.078. The neighbor-joining (NJ) tree showed that the original plants of Shi-Liang tea species can be easily differentiated from closely related species. Distinct molecular differences were found between the secondary structures of ITS2 sequences from Shi-Liang tea and closely related species. The results in the present investigation suggested that the ITS2 could be an effective DNA marker to identify the original plants of Shi-Liang tea and their closely related species. These DNA barcodes can greatly benefit the supervision of the commercial circulation of She medicine. View Full-Text
Keywords: traditional She medicine; Shi-Liang tea; Chimonanthus; ITS2; identification traditional She medicine; Shi-Liang tea; Chimonanthus; ITS2; identification

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Ma, S.; Lv, Q.; Zhou, H.; Fang, J.; Cheng, W.; Jiang, C.; Cheng, K.; Yao, H. Identification of Traditional She Medicine Shi-Liang Tea Species and Closely Related Species Using the ITS2 Barcode. Appl. Sci. 2017, 7, 195.

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