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Appl. Sci. 2017, 7(1), 59;

Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells

Department of Large Animal Clinical Sciences, Marion duPont Scott Equine Medical Center, Virginia-Maryland College of Veterinary Medicine, Virginia Tech, Leesburg, VA 20176, USA
Department of Mechanical Engineering, College of Engineering, Virginia Tech, Blacksburg, VA 24061, USA
Author to whom correspondence should be addressed.
Academic Editor: Daniel X.B. Chen
Received: 15 December 2016 / Revised: 21 December 2016 / Accepted: 23 December 2016 / Published: 6 January 2017
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Tendon is commonly injured, heals slowly and poorly, and often suffers re-injury after healing. This is due to failure of tenocytes to effectively remodel tendon after injury to recapitulate normal architecture, resulting in poor mechanical properties. One strategy for improving the outcome is to use nanofiber scaffolds and mesenchymal stem cells (MSCs) to regenerate tendon. Various scaffold parameters are known to influence tenogenesis. We designed suspended and aligned nanofiber scaffolds with the hypothesis that this would promote tenogenesis when seeded with MSCs. Our aligned nanofibers were manufactured using the previously reported non-electrospinning Spinneret-based Tunable Engineered Parameters (STEP) technique. We compared parallel versus perpendicular nanofiber scaffolds with traditional flat monolayers and used cellular morphology, tendon marker gene expression, and collagen and glycosaminoglycan deposition as determinants for tendon differentiation. We report that compared with traditional control monolayers, MSCs grown on nanofibers were morphologically elongated with higher gene expression of tendon marker scleraxis and collagen type I, along with increased production of extracellular matrix components collagen (p = 0.0293) and glycosaminoglycan (p = 0.0038). Further study of MSCs in different topographical environments is needed to elucidate the complex molecular mechanisms involved in stem cell differentiation. View Full-Text
Keywords: tendon; differentiation; topography; scaffolds; nanofiber tendon; differentiation; topography; scaffolds; nanofiber

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Popielarczyk, T.L.; Nain, A.S.; Barrett, J.G. Aligned Nanofiber Topography Directs the Tenogenic Differentiation of Mesenchymal Stem Cells. Appl. Sci. 2017, 7, 59.

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