Next Article in Journal
Energy-Harvesting Performances of Two Tandem Piezoelectric Energy Harvesters with Cylinders in Water
Next Article in Special Issue
Generating Radially and Azimuthally Polarized Beams by Using a Pair of Lateral Displacement Beamsplitters
Previous Article in Journal
Dynamic Similitude Design Method of the Distorted Model on Variable Thickness Cantilever Plates
Previous Article in Special Issue
A Self-Testing Platform with a Foreground Digital Calibration Technique for SAR ADCs
Article Menu

Export Article

Open AccessArticle
Appl. Sci. 2016, 6(8), 229;

Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection

Metal Industries Research & Development Centre, Kaohsiung 811, Taiwan
Department of Mechanical and Electro-Mechanical Engineering, National Sun Yat-sen University, Kaohsiung 804, Taiwan
Author to whom correspondence should be addressed.
Academic Editors: Teen-Hang Meen and Totaro Imasaka
Received: 9 June 2016 / Revised: 4 August 2016 / Accepted: 9 August 2016 / Published: 13 August 2016
Full-Text   |   PDF [4322 KB, uploaded 13 August 2016]   |  


A high performance diascopic illumination configuration is presented for the simultaneous detection of cells and particles with different sizes and different fluorescence labels in a microchannel. In the proposed approach, the cells/particles are illuminated by an objective-type dark-field condenser equipped with a low-cost tungsten light source and are then characterized by extracting the side-scatter, absorbance, and fluorescence signals from the spectra obtained by a ultraviolet-visible-near infrared (UV-Vis-NIR) spectrometer. A modified computation model is adopted to improve the capability for discriminating more fluorescence dyes simultaneously. The feasibility of the proposed detection configuration is demonstrated by counting and classifying a mixed sample of green, red, and crimson fluorescent-labeled particles and non-labeled particles with various dimensions. The suitability of the proposed system for real-world cytometry applications is then evaluated by classifying a mixed bio-sample comprising of gastric epithelial (AGS) cells stained with Trypan-blue and Erythrosin-bluish dye, respectively. The results show that the cytometer enables the efficient detection, identification, and classification of mixed bio-samples without the need for spatial filters or delicate optical components. Consequently, the proposed system has significant potential for high-performance micro-flow cytometry applications. View Full-Text
Keywords: micro-flow cytometry; multispectral detection; dark-field illumination; diascopic configuration micro-flow cytometry; multispectral detection; dark-field illumination; diascopic configuration

Graphical abstract

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

Share & Cite This Article

MDPI and ACS Style

Lin, S.-W.; Lin, C.-H. Chip-Based Cytometry Illuminated by a Blade-Shape Continuous Light for Multispectral Detection. Appl. Sci. 2016, 6, 229.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics



[Return to top]
Appl. Sci. EISSN 2076-3417 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top