Review Reports

Round 1

Reviewer 1 Report

In this manuscript, the authors summarized the medical applications of single molecule electrical measurement using nanodevices. This review provides useful information on the recent progress of nanodevice-based single-molecule electrical measurement technology in the field of personalized medicine. Specific comments,

1. For providing complete information to readers, the recently published review articles such as Mater.2020, 32, 2002219; Adv. Sci.2021,8, 2102756; Analyst, 2021, 146, 4111, Develop Growth Differ. 2019;61:316; Nature biotechnology 39, pages1348–1365 (2021), etc. should be included in the manuscript.
2. Are there any nanodevices which have been already approved for clinical application? The challenges of nanodevices in the clinical applications should be mentioned in the ‘ Discussion and Future prospects’ section.
3. The English writing should be checked carefully. In particular, the description of columns in Tables.

Author Response

I would like to express many thanks to the referees for his or her valuable and helpful comments. I have studied the comments carefully and made the following revisions. Please see the attachment.

Author Response File: Author Response.docx

Reviewer 2 Report

This manuscript provides a review of single molecule detection in disease diagnostics. The author covers primarily nanopore and nanogap devices but has overlooked nanopipette devices. See references below.

Current Rectification with Poly-l-Lysine-Coated Quartz Nanopipettes, S Umehara, N Pourmand, CD Webb, RW Davis, K Yasuda, M Karhanek, Nano letters 6 (11), 2486-2492, 2006

Single DNA molecule detection using nanopipettes and nanoparticles, M Karhanek, JT Kemp, N Pourmand, RW Davis, CD Webb, Nano letters 5 (2), 403-407, 2005

Label-free biosensing with functionalized nanopipette probes, S Umehara, M Karhanek, RW Davis, N Pourmand, Proceedings of the National Academy of Sciences 106 (12), 4611-4616, 2009

Functionalized nanopipettes: toward label-free, single cell biosensors, P Actis, AC Mak, N Pourmand, Bioanalytical reviews 1 (2-4), 177-185, 2010

Functionalized quartz nanopipette for intracellular superoxide sensing: a tool for monitoring reactive oxygen species levels in single living cell, RE Ozel, G Bulbul, J Perez, N Pourmand, ACS sensors 3 (7), 1316-1321, 2018

Nanopipettes as monitoring probes for the single living cell: State of the art and future directions in molecular biology, G Bulbul, G Chaves, J Olivier, RE Ozel, N Pourmand, Cells 7 (6), 55, 2018

Nanopipettes—The past and the present, J Stanley, N Pourmand, APL Materials 8 (10), 100902, 2020

Author Response

I would like to express many thanks to the referees for his or her valuable and helpful comments. I have studied the comments carefully and made the following revisions. Please see the attachment

Author Response File: Author Response.docx

Round 2

Reviewer 1 Report

The revised manuscript could be published as it.