Nanodevices for Biological and Medical Applications: Development of Single-Molecule Electrical Measurement Method
Round 1
Reviewer 1 Report
In this manuscript, the authors summarized the medical applications of single molecule electrical measurement using nanodevices. This review provides useful information on the recent progress of nanodevice-based single-molecule electrical measurement technology in the field of personalized medicine. Specific comments,
- For providing complete information to readers, the recently published review articles such as Mater.2020, 32, 2002219; Adv. Sci.2021,8, 2102756; Analyst, 2021, 146, 4111, Develop Growth Differ. 2019;61:316; Nature biotechnology 39, pages1348–1365 (2021), etc. should be included in the manuscript.
- Are there any nanodevices which have been already approved for clinical application? The challenges of nanodevices in the clinical applications should be mentioned in the ‘ Discussion and Future prospects’ section.
- The English writing should be checked carefully. In particular, the description of columns in Tables.
Author Response
I would like to express many thanks to the referees for his or her valuable and helpful comments. I have studied the comments carefully and made the following revisions. Please see the attachment.
Author Response File: 
Author Response.docx
Reviewer 2 Report
This manuscript provides a review of single molecule detection in disease diagnostics. The author covers primarily nanopore and nanogap devices but has overlooked nanopipette devices. See references below.
Current Rectification with Poly-l-Lysine-Coated Quartz Nanopipettes, S Umehara, N Pourmand, CD Webb, RW Davis, K Yasuda, M Karhanek, Nano letters 6 (11), 2486-2492, 2006
Single DNA molecule detection using nanopipettes and nanoparticles, M Karhanek, JT Kemp, N Pourmand, RW Davis, CD Webb, Nano letters 5 (2), 403-407, 2005
Label-free biosensing with functionalized nanopipette probes, S Umehara, M Karhanek, RW Davis, N Pourmand, Proceedings of the National Academy of Sciences 106 (12), 4611-4616, 2009
Functionalized nanopipettes: toward label-free, single cell biosensors, P Actis, AC Mak, N Pourmand, Bioanalytical reviews 1 (2-4), 177-185, 2010
Functionalized quartz nanopipette for intracellular superoxide sensing: a tool for monitoring reactive oxygen species levels in single living cell, RE Ozel, G Bulbul, J Perez, N Pourmand, ACS sensors 3 (7), 1316-1321, 2018
Nanopipettes as monitoring probes for the single living cell: State of the art and future directions in molecular biology, G Bulbul, G Chaves, J Olivier, RE Ozel, N Pourmand, Cells 7 (6), 55, 2018
Nanopipettes—The past and the present, J Stanley, N Pourmand, APL Materials 8 (10), 100902, 2020
Author Response
I would like to express many thanks to the referees for his or her valuable and helpful comments. I have studied the comments carefully and made the following revisions. Please see the attachment
Author Response File: Author Response.docx
Round 2
Reviewer 1 Report
The revised manuscript could be published as it.
