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Article

β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes

Kirin Central Research Institute, Kirin Holdings Company, Limited, 2-26-1 Muraoka-Higashi, Fujisawa, Kanagawa 251-8555, Japan
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Author to whom correspondence should be addressed.
Academic Editor: Malgorzata Ziarno
Appl. Sci. 2021, 11(7), 3034; https://doi.org/10.3390/app11073034
Received: 24 February 2021 / Revised: 24 March 2021 / Accepted: 25 March 2021 / Published: 29 March 2021
(This article belongs to the Special Issue Functional Dairy Products)
Astrocytes are known to regulate normal brain function. Monoamine oxidase B (MAO-B), an enzyme highly expressed in astrocytes, metabolizes dopamine (DA) and induces reactive oxygen species (ROS) production. We have previously reported that β-lactolin, a whey-derived glycine–threonine–tryptophan–tyrosine tetrapeptide, improves memory impairment in mice by regulating the dopaminergic system; however, the effects of β-lactolin on astrocytes remain unclear. Herein, we investigated the effects of β-lactolin on cultured murine astrocytes. First, we measured intracellular ROS production in lipopolysaccharide-stimulated reactive astrocytes treated with or without β-lactolin, and then determined the role of β-lactolin in DA metabolism in astrocytes by measuring MAO-B enzyme activity and the levels of DA, and its metabolites, in DA-pretreated astrocytes. We found that β-lactolin significantly suppressed ROS production in lipopolysaccharide-stimulated reactive astrocytes (p = 2.76 × 10−6), inhibited MAO-B activity (p = 2.65 × 10−2) and increased intracellular DA levels (p = 1.08 × 10−3), suggesting that β-lactolin could inhibit DA metabolism in astrocytes. These results illustrate the novel protective effects of β-lactolin on reactive astrocytes and suggest their involvement in the memory-improving effects of β-lactolin via the dopaminergic system. View Full-Text
Keywords: β-lactolin; β-lactoglobulin; whey; peptide; astrocytes; reactive oxygen species; monoamine oxidase B; dopamine β-lactolin; β-lactoglobulin; whey; peptide; astrocytes; reactive oxygen species; monoamine oxidase B; dopamine
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MDPI and ACS Style

Akiyama, S.; Ayabe, T.; Takahashi, C.; Ohya, R.; Ano, Y. β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes. Appl. Sci. 2021, 11, 3034. https://doi.org/10.3390/app11073034

AMA Style

Akiyama S, Ayabe T, Takahashi C, Ohya R, Ano Y. β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes. Applied Sciences. 2021; 11(7):3034. https://doi.org/10.3390/app11073034

Chicago/Turabian Style

Akiyama, Shiori, Tatsuhiro Ayabe, Chika Takahashi, Rena Ohya, and Yasuhisa Ano. 2021. "β-lactolin, a Monoamine Oxidase B Inhibitory Lactopeptide, Suppresses Reactive Oxygen Species Production in Lipopolysaccharide-Stimulated Astrocytes" Applied Sciences 11, no. 7: 3034. https://doi.org/10.3390/app11073034

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