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Review

The Administration of Probiotics against Hypercholesterolemia: A Systematic Review

by
Bhagavathi Sundaram Sivamaruthi
1,
Muruganantham Bharathi
1,
Periyanaina Kesika
1,
Natarajan Suganthy
2 and
Chaiyavat Chaiyasut
1,*
1
Innovation Center for Holistic Health, Nutraceuticals, and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand
2
Department of Nanoscience and Technology, Alagappa University, Karaikudi 630003, India
*
Author to whom correspondence should be addressed.
Appl. Sci. 2021, 11(15), 6913; https://doi.org/10.3390/app11156913
Submission received: 28 May 2021 / Revised: 16 July 2021 / Accepted: 24 July 2021 / Published: 27 July 2021
(This article belongs to the Special Issue Effects of Probiotics on Host Health)
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Abstract

:

Featured Application

The current review helps find a better probiotic strain or probiotic mixture to develop probiotic-based adjuvant therapy to control hypercholesterolemia.

Abstract

Hypercholesterolemia is a key factor in the progression of atherosclerosis and cardiovascular disease (CVD). CVD is a significant public health concern with a high death rate. Some of the main factors linked to CVD include genetics and lifestyle. Dyslipidemia has been one of the factors related to the onset of several CVD-related diseases. Several clinicopathological studies have shown a correlation between high cholesterol levels, particularly low-density lipoprotein cholesterol (LDL-c), and CVD development. Probiotics have received a lot of attention for various beneficial effects, especially their ability to reduce blood cholesterol in humans. Probiotics were shown in several investigations to affect hypercholesterolemia by influencing cholesterol biosynthesis. The current review focuses on the human dietary interventions with probiotics and their effects on CVD risk factors and hypercholesterolemia. The outcomes are debatable and consider various parameters such as probiotic strain, dosing frequency, therapeutic response, dietary changes, and so forth. As a result, probiotics have the propensity to become dietary supplements in moderate/severe hypercholesterolemic patients, which significantly reduces the CVD risk.

1. Introduction

Hypercholesterolemia is a widespread hereditary metabolic disease caused by elevated serum levels of low-density lipoprotein cholesterol (LDL-c), leading to premature coronary artery disease [1]. Hypercholesterolemia is one of the leading causes of CVD, and when treated appropriately, it can reduce the risk of CVD-related morbidity and death. The Adult Treatment Panel III (ATP III) of the National Cholesterol Education Program (NCEP) recommends LDL-c levels of 100 mg/dL are optimal and >160 is high [2]. It is predominantly caused by the mutation in low-density lipoprotein receptor (LDLR), apolipoprotein B-100 and proprotein convertase subtilisin/Kexin 9 (PCSK9), which leads to decreased LDL-c receptor absorption and evacuation from the circulation [3,4,5]. Due to the emphasis on lifestyle changes, most patients must use medications to achieve a sufficient decline in LDL-c levels. It also implies that any cholesterol-lowering medication should focus on LDL-c as the primary goal [6]. Statins are the most often prescribed treatment for hypercholesterolemia. Studies have shown that statins lower the risk of heart attack and mortality by 30–40% and lower LDL-c levels by 25–40% [7,8]. Statins are also combined with other small molecules such as ezetimibe, proprotein convertase subtilisin-kexin type 9 inhibitors, bempedoic acid, angiopoietin-like 3 protein inhibitors, and nutraceuticals to treat hypercholesterolemic patients. At the same time, it develops intolerance [9,10,11,12]. However, statin usage is now widely known to be linked to various side effects and symptoms, including myositis, myalgia, rhabdomyolysis, cognitive impairment, liver dysfunction, neuropathy, pancreatitis, sexual development, and semen parameters [13,14,15].
Ayurveda is one of the oldest well-known medical practices in the world. Ayurvedic formulations are prepared based on the patient’s diet, behavioral changes, patient assessment, detoxification, and rejuvenation condition [16,17,18]. The most widely used herbs to reduce cholesterol are garlic, guggulu, and arjuna [19,20,21]. However, there is no clear evidence for the direct correlation between herbs and hypercholesterolemia [21]. The extracts of red yeast rice (RYR) are currently the most effective cholesterol-lowering nutraceuticals, containing monacolin K, a reversible inhibitor of 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase. It improves the reduction of LDL-c associated with the improved function of the endothelium and arterial stiffness in CVD [22,23]. The prevalent use of RYR medications is still specific, and they should not be included in place of statins or other LDL-c lowering pharmacological approaches as the mainstay potential therapy for efficiently lowering CVD risk, especially in patients with high or very high CVD risk, as current guidelines highly suggested [24]. Due to medications’ highest prices and side effects, the desire to use probiotics or mix probiotics treatment as an efficient solution.
In 2002, the World Health Organization described probiotics as “live microorganisms that, when consumed in sufficient quantities, offer the host a therapeutic benefit.” Several scientists from the International Scientific Association formally approved the definition for probiotics and prebiotics [25]. There may be some advantages of daily consumption of microorganisms, such as general health protection and disease prevention [26]. The first microorganism-rich diet is breast milk, containing up to 107 microbes per milliliter [27]. The emergence of chronic diseases has been attributed to decreasing everyday consumption of microbial-rich foods [28,29]. According to growing research, probiotics have been shown to decrease LDL-c and enhance the LDL/HDL ratio and lower blood pressure, inflammatory cytokines, insulin sensitivity, and mass index [30,31]. The administration of Lactobacillus, a well-known probiotic, may be considered as the essential treatment method for lipid-lowering effects and urinary tract infections [32,33]. As an adjuvant and complementary medicine, probiotics are often supplemented in the diet to manage metabolic and intestinal diseases [34]. The probiotic bacteria Bifidobacteria and lactobacilli may be used as prophylactic or therapeutic agents towards lowering cholesterol levels, enteric pathogens, and control of metabolic disorders in infants and adults. Several strains of the Lactobacillus acidophilus, L. plantarum, and Lactococcus lactis led to a reduced serum cholesterol level due to their ability to assimilate cholesterol available in vitro [35]. The evidence supporting probiotic microorganisms being given prophylactically to preterm newborns to avoid necrotizing enterocolitis, late-onset infection, and mortality is substantial. Still, it’s restricted to the L. reuteri DSM 17938 species [36].
Probiotic preparations with or without pharmaceutical formulations have been shown to enhance the health of menopausal women [37]. Therefore, probiotic bacteria may benefit human health and maintain a healthy microbial gut [38]. However, additional research is needed to understand how probiotics may benefit the cardiovascular system by decreasing LDL-c substantially and figure out any potential detrimental health effects [39]. This study provides updated information about the reported beneficial effects of probiotic supplementations in hypercholesterolemic subjects.

2. Materials and Methods

The literature search was made in PubMed and web of science using a mixture of keywords’ probiotics and hypercholesterolemia’ and ‘hypercholesterolemia and cardiovascular disease.’ The scientific reports were collected, which were published till 24 February 2021. The Method PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) was used in this systematic review to set the selection criteria of the collected clinical trials. Clinical studies have been included in the study concerning subjects with high and moderate CVD and hypercholesterolemia without the possibility of CVD.

3. Results

From 24 clinical trials, 17 full-text articles were considered after implementing the exclusion criteria and represented in Figure 1.
The details of the reported studies on the influence of probiotics on hypercholesterolemia have been summarized in Table 1. The studies revealed the pilot/randomized controlled trial method to investigate the effects of probiotics in hypercholesterolemic patients. Lactobacillus (in nine studies), Bifidobacterium (in eight studies), and Enterococcus (in two studies) were the most widely studied genera in these reports. Saccharomyces and Propionibacterium were used in one study. The studies were planned to assess the effects of probiotics on the lipid profile, considered a crucial causative agent of CVD.
The 33 patients with low and without CVD risk were tested with Bifidobacterium longum BB536 and red yeast rice, which reduced the LDL-c level. The circulating levels of lathosterol in plasma, a marker of cholesterol synthesis, were also significantly reduced (p = 0.0206). It revealed that B. longum BB536 and red yeast rice affect the lathosterol biosynthesis [40]. L. plantarum ECGC 13110402 was administrated with the proper regular diet to 23 people with normal to mildly hypercholesterolemia, which showed a significant difference in the LDL-c concentrations. L. plantarum ECGC 13110402 has high bile salt hydrolase activity, which reduced the LDL-c level and may lower the CVD risk [41]. The isoflavone-supplemented soy product fermented with Enterococcus faecium CRL 183 and L. helveticus 416 was administered to 17 patients. At the end of 42 days, the outcomes were observed that the probiotic intervention significantly reduced the LDL-c level (up to 14.8%) [42].
After eight weeks of daily supplementation to adults with hypercholesterolemia (n = 11) found that Saccharomyces boulardii var. boulardii CNCM I-1079 significantly reduced (about 15.5%) remnant lipoprotein particles. However, there are no significant changes in total cholesterol (TC), LDL-c, HDL-c, triglycerides (TG), TC/HDL-c, and non-HDL-c [43]. L. reuteri NCIMB 30242 with bile salt hydrolase functionality decreased the LDL-c and increased the circulating bile acid. L. reuteri NCIMB 30242 also increases the serum 25-hydroxyvitamin D (25.5%), which significantly reduces the risk of osteoporosis, CVD, diabetes, and some cancers [44,45]. It may reduce CVD risk and other chronic diseases [46]. However, further experiments are needed to warrant the statement.
Table
Table 1. The outcomes of probiotic intervention in hypercholesterolemic subjects.
Table 1. The outcomes of probiotic intervention in hypercholesterolemic subjects.
AuthorsType of the StudyProbiotics UsedDuration DoseStudy SubjectsThe Outcome of the Study
Ruscica et al., 2019 [40]RCTLactoflorene Colesterolo®12 weeks1 sachet/dayLow CVD risk and LDL-c (130–200 mg/dL) subjects; 17 females, 16 males; Age = 18–70 years; Probiotic group, n = 16; Placebo group, n = 17Improved the proatherogenic lipid profile
Costabile et al., 2017 [41]RCTLactobacillus plantarum ECGC 1311040212 weeks 4 × 109 CFU/day Normal to mild hypercholesterolemic subjects; 34 females, 15 males; Age = 30–65 years; Average BMI = 26.43 kg/m2; Probiotic group, n = 23; Placebo group, n = 23Well-tolerated by the subjects, ECGC 13110402 could reduce CVD risk
Cavallini et al., 2016 [42]RCTIsoflavone-supplemented soy product fermented with Enterococcus faecium CRL 183 and Lactobacillus helveticus 41642 days11 × 109 CFU/day
(or)
Probiotics with ~100 mg of total isoflavones		Mild hypercholesterolemic male subjects (TC: > 5.17 mmol/L); Age = 45–48; Probiotic soy product (SP) group, n = 17; Isoflavone supplemented probiotic soy product (ISP) group, n = 17; unfermented soy product (USP) placebo group, n = 15Reduced the CVD risk in ISP group
Ryan et al., 2015 [43]PilotSaccharomyces boulardii var. boulardii CNCM I-10798 weeks5.6 × 1010 CFU twice/dayHypercholesterolemic subjects; n = 11; 1 female, 10 males; Age = 38.27 ± 8.52 years; Average BMI = 28.02 kg/m2Lowered remnant lipoprotein level
Jones et al., 2013 [44]RCTL. reuteri NCIMB 302429 weeks2 × 109
CFU twice/day		Healthy hypercholesterolemic subjects (LDL-c > 3.4 mmol/L; TG < 4.0 mmol/L; Range of BMI = 22–32 kg/m2); Probiotic group, n = 66 (38 females, 28 males; Average age = 50.48 ± 14.03 years); placebo group, n = 61 (34 females, 27 males; Average age = 47.59 ± 12.88 years)Increased the circulating 25-hydroxyvitamin D in the probiotic group
Martoni et al., 2015 [46]Pilot, RCTLactobacillus reuteri NCIMB 302424 weeksDose for hypercholesterolemic subjects:
3 × 109 CFU/day for 1st week; 3 × 109 CFU twice/day for 2nd week; 6 × 109 CFU twice/day for 3rd week; 9 × 109 CFU twice/day for 4th week.
Dose for healthy
normocholesterolemic subjects:
6 × 109 CFU twice/day for 4-week intervention period		Hypercholesterolemic subjects (LDL-C > 3.4 mmol/L, TG < 4.0 mmol/L, Range of BMI = 23.0–32.5 kg/m2 and total BA < 10 mmol/L), n = 10 (males and
females between 20–75 years old); Healthy
normocholesterolemic male subjects (LDL-C < 3.4 mmol/L, average age = 37.75 ± 8.26 years), n = 4		Significantly influence bile acids metabolism
Guardamagna et al., 2014 [47]RCTBifidobacterium animalis spp. lactis MB 2409, B. bifidum MB 109, and B. longum spp. longum BL0412 weeks1 × 109
CFU each strain/day		Dyslipidemic children; Probiotic group, n = 37 (22 females, 15 males; Average age = 11.1 ± 1.8 years; Average BMI = 18.9 ± 4.5 kg/m2); placebo group, n = 36 (21 females, 15 males; Average age = 11.4 ± 2.4 years; Average BMI = 19.6 ± 3.4 kg/m2)Slightly improved the lipid profile in the probiotic group
Fuentes et al., 2013 [48]RCTL. plantarum CECT 7527, L. plantarum CECT 7528 and L. plantarum CECT 752912 weeks1.2 × 109 CFU/dayHypercholesterolemic subjects (Age = 18–65 years); Probiotic group, n = 30, Average BMI = 25.9 ± 0.45 kg/m2; Placebo group, n = 30, Average BMI = 26.0 ± 0.44 kg/m2Reduced the level of TC, LDL-c, and oxidized LDL-c in the probiotic group.
Reduced the CVD risk
Moroti et al., 2012 [49]RCTL. acidophilus, Bifidobacterium bifidum, and Oligofructose30 days108 CFU/mL/200 mL/day; 2 g oligofructose/dayElderly people with type 2 diabetes mellitus (total cholesterol > 200 mg/dL; TG > 150 mg/dL; glycemia > 110 mg/dL); Synbiotic group, n = 9, Average age = 55.47 ± 2.0 years, Average BMI = 27.70 ± 0.78 kg/m2; Placebo group, n = 9, Average age = 56.89 ± 1.7 years, Average BMI = 28.21 ± 0.85 kg/m2Significantly increased the HDL and decreased the glycemia in the synbiotic group.
Hlivak et al., 2005 [50]RCTEnterococcus faecium M-74 and selenium (Se)60 weeksTwo × 109 CFU/day; 50 µg of SeHypercholesterolemic subjects Probiotic group, n = 20 (17 females, 3 males; Average age = 75.35 ± 1.49 years; Average BMI = 29.40 ± 0.86 kg/m2); placebo group, n = 18 (14 females, 4 males; Average age = 78.05 ± 1.68 years; Average BMI = 29.08 ± 1.14 kg/m2)Reduced serum cholesterol level in the probiotic group
Kiessling et al., 2002 [51]Cross-over studyControl yogurt containing S. thermophilus, L. lactis);
Synbiotic (Probiotic Yogurt containing S. thermophilus, L. lactis, L. acidophilus 145, B. longum 913, and oligofructose)		21 weeks; Group 1 received control yogurt during the 1st period followed by synbiotic during 2nd period followed by control yogurt during 3rd period;
Group 2 received control yogurt during the 1st and 2nd period followed by synbiotic during 3rd period; Each period duration is seven weeks.		300 g/dayHypercholesterolemic (n = 14 females; Average LDL-C = 5.7 ± 1.6 mmol/L, Average TG = 1.3 ± 0.6 mmol/L, Average BMI = 24.2 ± 2.9 kg/m2, Average TC = 7.6 ± 1.6 mmol/L), and Normocholesterolemic (n = 15 females; Average LDL-C = 4.1 ± 0.4 mmol/L, Average TG = 0.9 ± 0.4 mmol/L, Average BMI = 23.3 ± 3.4 kg/m2, Average TC = 5.7 ± 0.6 mmol/L) subjects; Group 1, n = 18; Group 2, n = 11;Long term consumption of yogurt (control and synbiotic) of both groups increased the serum HDL-c level
Ataie-Jafari et al., 2009 [52]RCTProbiotic yogurt containing L. acidophilus and
Bifidobacterium lactis, S. thermophilus, L. delbrueckii ssp. Bulgaricus;
Regular yogurt containing S. thermophilus, L. delbrueckii ssp. Bulgaricus		Six weeks3 × 100 g/dayHealthy hypercholesterolemic subjects (Average BMI = 26.1 ± 2.9 kg/m2, TC = 5.68 ± 0.70 mmol/L,
LDL-C = 3.60 ± 0.77 mmol/L, TG = 2.26 ± 0.84 mmol/L); Probiotic yogurt group, Ordinary yogurt group; n = 7 (5 females, 2 males) in each group		Significantly reduced the TC level in the probiotic yogurt group
Xiao et al., 2003 [53]Pilot studyControl yogurt containing S. thermophilus, L. delbrueckii ssp. Bulgaricus;
Probiotic yogurt containing S. thermophilus, L. delbrueckii ssp. Bulgaricus and B. longum BL1		Four weeks~108 CFU/mL of B. longum BL1; 300 mL/dayHealthy moderate hypercholesterolemic male subjects; Control yogurt group, n = 16 (Age = 28–60 years; TC = 223–277 mg/dL); Probiotic yogurt group, n = 16 (Age = 31–59 years; TC = 221–272 mg/dL)Lowered serum cholesterol level in the probiotic yogurt group
Larkin et al., 2009 [54]Cross-over study (study of crossover groups of probiotic cohort or prebiotic cohort)Soy (S) diet; Probiotic yogurt (PY) containing L. acidophilus, B. bifidum, and Lactobacillus GG; Control yogurt (CY); Prebiotic (resistant starch); 14 weeks; (2-week wash-in period before the study) two 5-week
dietary periods (S + CY, S + PY in probiotic Group 1 and vice versa in probiotic Group 2) (S, S + RS in prebiotic Group 1 and vice versa in prebiotic Group 2) separated by a 4-week washout period		1 × 108 CFU/dayMild hypercholesterolemic subjects (men and post-menopausal women) (TC > 5.5 mmol L/1, LDL-c > 3.0 mmol L/1); Age ≥ 45 years;
Probiotic group 1 (4 females, 6 males); Probiotic group 2 (2 females, 4 males); Prebiotic group 1 (3 females, 6 males); Prebiotic group 2 (3 females, 3 males)		Significant reduction of TC in probiotic groups. Reduction in TC and LDL-c in prebiotic groups. The lipid-lowering effect observed in probiotic and prebiotic groups are not associated with the involvement of isoflavone
Greany et al., 2004 [55]RCTSPI (or) SPI + L. acidophilus DDS-1, and B. longum (or) MPI (or) MPI + L. acidophilus DDS-1, and B. longumSix weeks109 CFU/day and 26 ± 5 g milk proteinsHealthy mildly hypercholesterolemic (HC) postmenopausal women (n = 25) (TC > 5.17–6.59 mmol/L, 200–255 mg/dL; Average age = 57.8 ± 1.2; Average BMI = 25.7 ± 1.0 kg/m2); Normocholesterolemic (NC) postmenopausal women (n = 12) (TC < 5.17 mmol/L, <200 mg/dL; Average age = 57.0 ± 1.5; Average BMI = 24.7 ± 0.9 kg/m2) subjects; n (based on diet) for both soy protein diet and probiotic diet (HC, n = 48; NC, n = 23), and for both milk protein diet and probiotic free diet (HC, n = 48; NC, n = 24).Soy protein improved the plasma lipid profile, while probiotic intervention does not show any positive response
RCT: Randomized controlled trial; CVD: Cardiovascular disease; Lactoflorene Colesterolo®: 1 × 1010 UFC Bifidobacterium longum BB536, Red yeast rice extract (10 mg monacolin K), 16 mg niacin, 20 mg coenzyme Q10; TC: Total cholesterol; LDL-c: Low-density lipoprotein-cholesterol; HDL-c: High-density lipoprotein-cholesterol; TG = Triglyceride; BMI = Body mass index; Probiotic yogurt: Streptococcus thermophilus and Lactobacillus delbrueckii spp. Bulgaricus, and L. acidophilus and B. lactis were used to prepare probiotic yogurt; SPI: Soy protein isolate; MPI: Milk protein isolate.
The probiotic mixture comprised of B. lactis MB 2409, B. bifidum MB 109B, and B. longum BL04 showed a significant decrease in TC, LDL-c, and TG and an increase in HDL-c levels in dyslipidemic children [47]. Fuentes et al. reported that the supplementation of the probiotic mixture contains three strains of L. plantarum which significantly reduced the TC (13.6%), LDL-c, and oxidized LDL-c levels in hypercholesterolemic adults. It significantly reduces the CVD risk in hypercholesterolemic adults [48]. Moroti et al. described that the supplementation of a synbiotic mixture contains L. acidophilus, B. bifidum, and oligofructose effectively increased HDL-c and reduced the fasting glycemia in elderly people with type 2 diabetes mellitus [49]. The oral administration of E. faecium M-74 for one year reduces the LDL-c concentration significantly [50].
Iran Dairy Industries uses Streptococcus thermophilus and Lactobacillus delbrueckii subsp. Bulgaricus for making their regular yogurt. Along with those two bacteria, L. acidophilus and B. lactis were used as a starter to prepare probiotic yogurt. The probiotic yogurt had a cholesterol-lowering effect in healthy hypercholesterolemic subjects. Likewise, the yogurt fermented with L. acidophilus and B. longum did not lower the TC and LDL-c but increased the level of HDL-c significantly, which helped to maintain the LDC-c and HDL-c ratio [51]. The detailed mechanism discussing crucial probiotics used in cholesterol-lowering effects was not elucidated fully. However, the study needs further clarifications using more volunteers and extended study duration [52].

4. Discussion

The results were correlated with the study of milk products fermented with B. longum, which reduces the serum lipids in healthy moderate hypercholesterolemic male adults [53]. The isoflavone-supplemented soy product fermented with E. faecium CRL 183 and L. helveticus 416 significantly improves the lipid profile in moderately hypercholesterolemic men subjects. The fermented soy product has an anti-inflammatory effect and activates estrogen-receptor complex mechanisms, thereby reducing the LDL-c level. It reduces the serum LDL-c level, and no significant changes were noted in HDL-c and TG levels [43]. The reported results were established previously by Larkin et al. [54] and Greany et al. [55]. However, this study does not include women subjects to estimate the estrogen effects in women. In addition, pickled mustard greens fermented with lactic acid bacteria were beneficial for lowering cholesterol [56]. The possible benefit of a natural health mixture containing B. longum BB536 and RYR (Red Yeast Rice) extract involves two different pathways to facilitate LDL-c and TC reduction. RYR extracts inhibit the liver’s cholesterol production, and B. longum BB536, by its high bile salt hydrolase function, reduces intestinal cholesterol absorption [40]. In addition, the expression of LXRa and LXRb/NPC1L1 genes plays a critical role in the absorption of intestinal cholesterol in vitro [57,58,59]. L. plantarum ECGC supports the bile acid function, which reduces the cholesterol in mildly hypercholesterolemic adults without any impact on gastrointestinal and gut microbiota that can be possibly the best natural cholesterol-lowering supplement [42]. However, available data indicate that the study does not target severe hypercholesteremic patients to claim the results are accurate.
S. boulardii var. boulardii CNCM I-1079 supplementation lowered remnant lipoprotein. TG-rich lipoproteins are linked to the incidence and development of coronary artery disease, regardless of LDL-c levels [43]. However, the precise lipoprotein lowering mechanisms of S. boulardii is undisclosed, and the safety and tolerance of the strain were also not evaluated in this study. The safety risk management criteria are required to measure the safety of probiotics. However, due to the wide variety of microorganisms, it is also important to determine the unique hazards connected with each probiotic, including the risk factors associated with the host and the complex interaction between the probiotic, host, and nutrition constituents [60,61].
L. reuteri NCIMB 30242 was found to raise bloodstream bile acid levels involved in several metabolic activities, including energy balance, lipid metabolism, and inflammatory control [62,63]. The administration of Bifidobacterium species claimed to be involved in the control of dyslipidemia in hypercholesterolemic children by improving lipid profile [48]. However, to elucidate the precise molecular mechanisms of action of Bifidobacterium in dyslipidemic children, further studies and clinical examination are needed.
L. reuteri NCIMB lowers cholesterol and reduced sterol absorption. Authors have also analyzed the increased circulation of 25-hydroxyvitamin D in serum during L. reuteri NCIMB administration and found that the level of vitamin D was increased at the end of the study. The increased vitamin D level improves the proper intestinal colonization of probiotics, prevents pathogenic bacterial invasion, reduces chronic inflammation, and maintains enterocyte cell integrity [45,64,65]. Women who consumed yogurt containing L. acidophilus 145 and B. longum 913 regularly for six months had higher HDL-c levels and improved LDL-c/HDL-c ratio [52]. Fukushima and Nakano reported that a probiotic mixture diet containing Bacillus, Lactobacillus, Streptococcus, Clostridium, Saccharomyces, and Candida decreased cholesterol synthesis in rats’ liver. The L. acidophilus strains removed cholesterol by binding to the intestine surface area, making it less obtainable for absorption in the intestine [66]. Likewise, L. plantarum KCTC3928 and L. acidophilus ATCC 43121 enhanced the conversion of HMG-CoA and cholesterol to mevalonate and bile acid, respectively, reducing the LDL-c level in hypercholesterolemic patients [67,68,69].
The probiotic mixture of L. plantarum CECT 7527, 7528, and 7529 improves the cholesterol profile and reduces the CVD risk in hypercholesteremic patients [48]. However, the over-growth probiotics mixture may lead to gut dysbiosis, affecting physiology, gut-brain axis function, and other secondary disease outcomes in the host [70,71]. Further studies are needed to warrant the tolerance and safety of long-term consumption of a mixture of L. plantarum strains. The consumption of L. acidophilus, B. bifidum, and oligofructose increases the HDL-c level, reduces CVD risk, and significantly reduces the blood sugar level [49]. Ataie-Jafari et al. stated that probiotic yogurt consumption significantly decreases the total lipid content and reduces the CVD risk [51].
The administration of E. faecium M-74 enriched with selenium reduces the LDL-c level. However, no significant changes were noted in HDL-c and TG levels. Further studies are required to meet the safety and tolerance criteria [50].
The yogurt fermented with B. longum BL1 has the highest efficacy in lowering serum cholesterol in moderately hypercholesterolemic subjects [53]. However, there are no significant changes in HDL-c, TC, and TG levels. Furthermore, studies are needed to warrant the efficacy of B. longum BL1. Therefore, probiotics are the natural replacements that could be beneficial to improve the lipid profile and reduce the CVD risk without causing adverse effects, unlike statin drugs.

5. Conclusions

Our review concluded that probiotics against hypercholesterolemia could act as an alternative medicine to reduce the CVD risk and other diseases. The obtained clinical trailed data confirm the positive effect of probiotic consumption against hypercholesterolemia. As a result, additional research is needed to understand how probiotics can benefit the cardiovascular system and perhaps other ailments and test out any potential adverse health effects. The present literature review strongly recommends further clinical studies with various mixtures and dosages of probiotics in a greater number of volunteers. Finally, it appears that the potential of probiotics to decrease cholesterol depends on species and strain. L. acidophilus was found to reduce cholesterol levels. Similarly, a fermented milk product containing B. longum 913 and BL1 reduced the LDL-c cholesterol levels in the blood. The L. plantarum and L. reuteri lowers LDL-c levels while increasing vitamin D synthesis. Therefore, the strains of L. acidophilus, B. longum, and L. plantarum used in this study may have hypocholesterolemic properties.

Author Contributions

Conceptualization, B.S.S., M.B. and C.C.; methodology, M.B.; software, M.B.; validation, M.B. and C.C.; formal analysis, M.B.; investigation, M.B. and B.S.S.; resources, C.C.; data curation, M.B.; writing—original draft preparation, B.S.S., P.K. and M.B.; writing—review and editing, B.S.S., P.K., N.S., C.C. and M.B.; supervision, C.C.; project administration, C.C. and B.S.S.; funding acquisition, C.C. All authors have read and agreed to the published version of the manuscript.

Funding

This research received no external funding.

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The data presented in this study are available within the article.

Acknowledgments

The authors gratefully acknowledge the Faculty of Pharmacy, Chiang Mai University, Chiang Mai for the support. The research was partially supported by the Chiang Mai University, Chiang Mai, Thailand.

Conflicts of Interest

The authors declare no conflict of interest.

References

  1. Santos, R.D.; Gidding, S.S.; Hegele, R.A.; Cuchel, M.A.; Barter, P.J.; Watts, G.F.; Baum, S.J.; Catapano, A.L.; Chapman, M.J.; Defesche, J.C.; et al. International atherosclerosis society severe familial hypercholesterolemia panel. Defining severe familial hypercholesterolaemia and the implications for clinical management: A consensus statement from the international atherosclerosis society severe familial hypercholesterolemia panel. Lancet Diabetes Endocrinol. 2016, 4, 850–861, Erratum in 2016, 4, e8. [Google Scholar] [PubMed]
  2. Iglseder, B.; Moroder, T.; Staffen, W.; Ladurner, G. High prevalence and undertreatment of hypercholesterolaemia in participants in a public stroke prevention programme in Austria. Clin. Drug Investig. 2005, 25, 709–717. [Google Scholar] [CrossRef] [PubMed]
  3. Koskinas, K.; Wilhelm, M.; Windecker, S. Current treatment of dyslipidaemia: PCSK9 inhibitors and statin intolerance. Swiss Med. Wkly. 2016, 146, w14333. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  4. Pasta, A.; Cremonini, A.L.; Pisciotta, L.; Buscaglia, A.; Porto, I.; Barra, F.; Ferrero, S.; Brunelli, C.; Rosa, G.M. PCSK9 inhibitors for treating hypercholesterolemia. Expert Opin. Pharmacother. 2020, 21, 353–363. [Google Scholar] [CrossRef] [PubMed]
  5. Karatasakis, A.; Danek, B.A.; Karacsonyi, J.; Rangan, B.V.; Roesle, M.K.; Knickelbine, T.; Miedema, M.D.; Khalili, H.; Ahmad, Z.; Abdullah, S.; et al. Effect of PCSK9 Inhibitors on Clinical Outcomes in Patients with Hypercholesterolemia: A Meta-Analysis of 35 Randomized Controlled Trials. J. Am. Heart Assoc. 2017, 6, e006910. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  6. Mannu, G.S.; Zaman, M.J.; Gupta, A.; Rehman, H.U.; Myint, P.K. Evidence of lifestyle modification in the management of hypercholesterolemia. Curr. Cardiol. Rev. 2013, 9, 2–14. [Google Scholar] [PubMed] [Green Version]
  7. Chauvin, B.; Drouot, S.; Barrail-Tran, A.; Taburet, A.M. Drug-drug interactions between HMG-CoA reductase inhibitors (statins) and antiviral protease inhibitors. Clin. Pharmacokinet. 2013, 52, 815–831. [Google Scholar] [CrossRef] [PubMed]
  8. Lee, M.M.Y.; Sattar, N.; McMurray, J.J.V.; Packard, C.J. Statins in the Prevention and Treatment of Heart Failure: A Review of the Evidence. Curr. Atheroscler. Rep. 2019, 21, 41. [Google Scholar] [CrossRef] [Green Version]
  9. Schimmenti, C.; Sucato, V.; Manzone, E.; Cancellieri, G.; Mortillaro, F.; Novo, G.; Galassi, A.R.; Venturella, F. Bempedoic acid as adjunct for traditional lipid-lowering therapy in patients with hyperlipidaemia. Coron. Artery Dis. 2021, 32, 340–344. [Google Scholar] [CrossRef]
  10. Jia, X.; Lorenz, P.; Ballantyne, C.M. Poststatin Lipid Therapeutics: A Review. Methodist DeBakey Cardiovasc. J. 2019, 15, 32–38. [Google Scholar]
  11. Diaconu, C.C.; Iorga, R.A.; Furtunescu, F.; Katsiki, N.; Stoian, A.P.; Rizzo, M. Statin intolerance: New data and further options for treatment. Curr. Opin. Cardiol. 2021, 36, 487–493. [Google Scholar] [CrossRef]
  12. Barrios, V.; Escobar, C.; Cicero, A.F.; Burke, D.; Fasching, P.; Banach, M.; Bruckert, E. A nutraceutical approach (Armolipid Plus) to reduce total and LDL cholesterol in individuals with mild to moderate dyslipidemia: Review of the clinical evidence. Atheroscler. Suppl. 2017, 24, 1–15. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  13. Selva-O’Callaghan, A.; Alvarado-Cardenas, M.; Pinal-Fernández, I.; Trallero-Araguás, E.; Milisenda, J.C.; Martínez, M.Á.; Marín, A.; Labrador-Horrillo, M.; Juárez, C.; Grau-Junyent, J.M. Statin-induced myalgia and myositis: An update on pathogenesis and clinical recommendations. Expert Rev. Clin. Immunol. 2018, 14, 215–224. [Google Scholar] [CrossRef]
  14. Li, X.; Lyu, P.; Ren, Y.; An, J.; Dong, Y. Arterial stiffness and cognitive impairment. J. Neurol. Sci. 2017, 380, 1–10. [Google Scholar] [CrossRef]
  15. Theocharidou, E.; Papademetriou, M.; Reklou, A.; Sachinidis, A.; Boutari, C.; Giouleme, O. The Role of PCSK9 in the Pathogenesis of Non-alcoholic Fatty Liver Disease and the Effect of PCSK9 Inhibitors. Curr. Pharm. Des. 2018, 24, 3654–3657. [Google Scholar] [CrossRef] [PubMed]
  16. Patwardhan, K. Promoting evidence-base for Ayurveda. J. Ayurveda Integr. Med. 2019, 30391–30392. [Google Scholar] [CrossRef] [PubMed]
  17. Gyawali, D.; Vohra, R.; Orme-Johnson, D.; Ramaratnam, S.; Schneider, R.H. A Systematic Review and Meta-Analysis of Ayurvedic Herbal Preparations for Hypercholesterolemia. Medicina 2021, 57, 546. [Google Scholar] [CrossRef] [PubMed]
  18. Singh, B.B.; Vinjamury, S.P.; Der-Martirosian, C.; Kubik, E.; Mishra, L.C.; Shepard, N.P.; Singh, V.J.; Meier, M.; Madhu, S.G. Ayurvedic and collateral herbal treatments for hyperlipidemia: A systematic review of randomized controlled trials and quasi-experimental designs. Altern. Ther. Health Med. 2007, 13, 22–28. [Google Scholar] [PubMed]
  19. Ried, K.; Toben, C.; Fakler, P. Effect of garlic on serum lipids: An updated meta-analysis. Nutr. Rev. 2013, 71, 282–299. [Google Scholar] [CrossRef]
  20. Donato, F.; Raffetti, E.; Toninelli, G.; Festa, A.; Scarcella, C.; Castellano, M. TRIGU Project Working Group. Guggulu and Triphala for the Treatment of Hypercholesterolaemia: A Placebo-Controlled, Double-Blind, Randomised Trial. Complement. Med. Res. 2021, 28, 216–225. [Google Scholar] [CrossRef]
  21. Bent, S. Herbal medicine in the United States: Review of efficacy, safety, and regulation: Grand rounds at University of California, San Francisco Medical Center. J. Gen. Intern. Med. 2008, 23, 854–859. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  22. Cicero, A.F.G.; Fogacci, F.; Banach, M. Red Yeast Rice for Hypercholesterolemia. Methodist DeBakey Cardiovasc. J. 2019, 15, 192–199. [Google Scholar]
  23. Cicero, A.F.G.; Fogacci, F.; Zambon, A. Red Yeast Rice for Hypercholesterolemia: JACC Focus Seminar. J. Am. Coll. Cardiol. 2021, 77, 620–628. [Google Scholar] [CrossRef] [PubMed]
  24. Gerards, M.C.; Terlou, R.J.; Yu, H.; Koks, C.H.; Gerdes, V.E. Traditional Chinese lipid-lowering agent red yeast rice results in significant LDL reduction but safety is uncertain—A systematic review and meta-analysis. Atherosclerosis 2015, 240, 415–423. [Google Scholar] [CrossRef] [Green Version]
  25. Gibson, G.R.; Hutkins, R.; Sanders, M.E.; Prescott, S.L.; Reimer, R.A.; Salminen, S.J.; Scott, K.; Stanton, C.; Swanson, K.S.; Cani, P.D.; et al. Expert consensus document: The International Scientific Association for Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of prebiotics. Nat. Rev. Gastroenterol. Hepatol. 2017, 14, 491–502. [Google Scholar] [CrossRef] [Green Version]
  26. Sanders, M.E.; Benson, A.; Lebeer, S.; Merenstein, D.J.; Klaenhammer, T.R. Shared mechanisms among probiotic taxa: Implications for general probiotic claims. Curr. Opin. Biotechnol. 2018, 49, 207–216. [Google Scholar] [CrossRef] [PubMed]
  27. Boix-Amorós, A.; Collado, M.C.; Mira, A. Relationship between milk microbiota, bacterial load, macronutrients, and human cells during lactation. Front. Microbiol. 2016, 7, 492. [Google Scholar] [CrossRef] [Green Version]
  28. Logan, A.C.; Katzman, M.A.; Balanzá-Martínez, V. Natural environments, ancestral diets, and microbial ecology: Is there a modern “paleo-deficit disorder”? Part II. J. Physiol. Anthropol. 2015, 34, 9. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  29. Kramer, A.; Bekeschus, S.; Bröker, B.M.; Schleibinger, H.; Razavi, B.; Assadian, O. Maintaining health by balancing microbial exposure and prevention of infection: The hygiene hypothesis versus the hypothesis of early immune challenge. J. Hosp. Infect. 2013, 83, S29–S34. [Google Scholar] [CrossRef]
  30. Markowiak, P.; Śliżewska, K. Effects of Probiotics, Prebiotics, and Synbiotics on Human Health. Nutrients 2017, 9, 1021. [Google Scholar] [CrossRef]
  31. Kim, Y.A.; Keogh, J.B.; Clifton, P.M. Probiotics, prebiotics, synbiotics and insulin sensitivity. Nutr. Res. Rev. 2018, 31, 35–51. [Google Scholar] [CrossRef] [PubMed]
  32. Sivamaruthi, B.S.; Kesika, P.; Chaiyasut, C. A mini-review of human studies on cholesterol-lowering properties of probiotics. Sci. Pharm. 2019, 87, 26. [Google Scholar] [CrossRef] [Green Version]
  33. Sivamaruthi, B.S.; Fern, L.A.; Rashidah Pg Hj Ismail, D.S.N.; Chaiyasut, C. The influence of probiotics on bile acids in diseases and aging. Biomed. Pharmacother. 2020, 128, 110310. [Google Scholar] [CrossRef] [PubMed]
  34. Kesika, P.; Sivamaruthi, B.S.; Chaiyasut, C. Do Probiotics Improve the Health Status of Individuals with Diabetes Mellitus? A Review on Outcomes of Clinical Trials. Biomed. Res. Int. 2019, 2019, 1531567. [Google Scholar] [CrossRef] [PubMed]
  35. Marras, L.; Caputo, M.; Bisicchia, S.; Soato, M.; Bertolino, G.; Vaccaro, S.; Inturri, R. The Role of Bifidobacteria in Predictive and Preventive Medicine: A Focus on Eczema and Hypercholesterolemia. Microorganisms 2021, 9, 836. [Google Scholar] [CrossRef]
  36. Underwood, M.A. Should we treat every infant with a probiotic? Minerva Pediatr. 2019, 71, 253–262. [Google Scholar] [CrossRef]
  37. Sivamaruthi, B.S.; Kesika, P.; Chaiyasut, C. Influence of probiotic supplementation on climacteric symptoms in menopausal women-a mini review. Int. J. Appl. Pharm. 2018, 10, 43–46. [Google Scholar] [CrossRef] [Green Version]
  38. Hemarajata, P.; Versalovic, J. Effects of probiotics on gut microbiota: Mechanisms of intestinal immunomodulation and neuromodulation. Ther. Adv. Gastroenterol. 2013, 6, 39–51. [Google Scholar] [CrossRef] [Green Version]
  39. Thushara, R.M.; Gangadaran, S.; Solati, Z.; Moghadasian, M.H. Cardiovascular benefits of probiotics: A review of experimental and clinical studies. Food Funct. 2016, 7, 632–642. [Google Scholar] [CrossRef]
  40. Ruscica, M.; Pavanello, C.; Gandini, S.; Macchi, C.; Botta, M.; Dall’Orto, D.; Del Puppo, M.; Bertolotti, M.; Bosisio, R.; Mombelli, G.; et al. Nutraceutical approach for the management of cardiovascular risk—A combination containing the probiotic Bifidobacterium longum BB536 and red yeast rice extract: Results from a randomized, double-blind, placebo-controlled study. Nutr. J. 2019, 18, 13. [Google Scholar] [CrossRef]
  41. Costabile, A.; Buttarazzi, I.; Kolida, S.; Quercia, S.; Baldini, J.; Swann, J.R.; Brigidi, P.; Gibson, G.R. An in vivo assessment of the cholesterol-lowering efficacy of Lactobacillus plantarum ECGC 13110402 in normal to mildly hypercholesterolaemic adults. PLoS ONE 2017, 12, e0187964. [Google Scholar] [CrossRef]
  42. Cavallini, D.C.; Manzoni, M.S.; Bedani, R.; Roselino, M.N.; Celiberto, L.S.; Vendramini, R.C.; de Valdez, G.; Abdalla, D.S.; Pinto, R.A.; Rosetto, D.; et al. Probiotic soy product supplemented with isoflavones improves the lipid profile of moderately hypercholesterolemic men: A randomized controlled trial. Nutrients 2016, 8, 52. [Google Scholar] [CrossRef] [Green Version]
  43. Ryan, J.J.; Hanes, D.A.; Schafer, M.B.; Mikolai, J.; Zwickey, H. Effect of the probiotic saccharomyces boulardii on cholesterol and lipoprotein particles in hypercholesterolemic adults: A single-arm, open-label pilot study. J. Altern. Complement. Med. 2015, 21, 288–293. [Google Scholar] [CrossRef] [Green Version]
  44. Jones, M.L.; Martoni, C.J.; Prakash, S. Oral supplementation with probiotic L. reuteri NCIMB 30242 increases mean circulating 25-hydroxyvitamin D: A post hoc analysis of a randomized controlled trial. J. Clin. Endocrinol. Metab. 2013, 98, 2944–2951. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  45. Bahrami, A.; Sadeghnia, H.R.; Tabatabaeizadeh, S.A.; Bahrami-Taghanaki, H.; Behboodi, N.; Esmaeili, H.; Ferns, G.A.; Mobarhan, M.G.; Avan, A. Genetic and epigenetic factors influencing vitamin D status. J. Cell Physiol. 2018, 233, 4033–4043. [Google Scholar] [CrossRef] [PubMed]
  46. Martoni, C.J.; Labbé, A.; Ganopolsky, J.G.; Prakash, S.; Jones, M.L. Changes in bile acids, FGF-19 and sterol absorption in response to bile salt hydrolase active L. reuteri NCIMB 30242. Gut Microbes 2015, 6, 57–65. [Google Scholar] [CrossRef] [Green Version]
  47. Guardamagna, O.; Amaretti, A.; Puddu, P.E.; Raimondi, S.; Abello, F.; Cagliero, P.; Rossi, M. Bifidobacteria supplementation: Effects on plasma lipid profiles in dyslipidemic children. Nutrition 2014, 30, 831–836. [Google Scholar] [CrossRef] [PubMed]
  48. Fuentes, M.C.; Lajo, T.; Carrión, J.M.; Cuñé, J. Cholesterol-lowering efficacy of Lactobacillus plantarum CECT 7527, 7528 and 7529 in hypercholesterolaemic adults. Br. J. Nutr. 2013, 109, 1866–1872. [Google Scholar] [CrossRef] [Green Version]
  49. Moroti, C.; Souza Magri, L.F.; de Rezende Costa, M.; Cavallini, D.C.; Sivieri, K. Effect of the consumption of a new symbiotic shake on glycemia and cholesterol levels in elderly people with type 2 diabetes mellitus. Lipids Health Dis. 2012, 11, 29. [Google Scholar] [CrossRef] [Green Version]
  50. Hlivak, P.; Odraska, J.; Ferencik, M.; Ebringer, L.; Jahnova, E.; Mikes, Z. One-year application of probiotic strain Enterococcus faecium M-74 decreases serum cholesterol levels. Bratisl Lek Listy 2005, 106, 67–72. [Google Scholar]
  51. Kiessling, G.; Schneider, J.; Jahreis, G. Long-term consumption of fermented dairy products over 6 months increases HDL cholesterol. Eur. J. Clin. Nutr. 2002, 56, 843–849. [Google Scholar] [CrossRef] [Green Version]
  52. Ataie-Jafari, A.; Larijani, B.; Alavi Majd, H.; Tahbaz, F. Cholesterol-lowering effect of probiotic yogurt in comparison with ordinary yogurt in mildly to moderately hypercholesterolemic subjects. Ann. Nutr. Metab. 2009, 54, 22–27. [Google Scholar] [CrossRef]
  53. Xiao, J.Z.; Kondo, S.; Takahashi, N.; Miyaji, K.; Oshida, K.; Hiramatsu, A.; Iwatsuki, K.; Kokubo, S.; Hosono, A. Effects of milk products fermented by Bifidobacterium longum on blood lipids in rats and healthy adult male volunteers. J. Dairy Sci. 2003, 86, 2452–2461. [Google Scholar] [CrossRef]
  54. Larkin, T.A.; Astheimer, L.B.; Price, W.E. Dietary combination of soy with a probiotic or prebiotic food significantly reduces total and LDL cholesterol in mildly hypercholesterolaemic subjects. Eur. J. Clin. Nutr. 2009, 63, 238–245. [Google Scholar] [CrossRef] [Green Version]
  55. Greany, K.A.; Nettleton, J.A.; Wangen, K.E.; Thomas, W.; Kurzer, M.S. Probiotic consumption does not enhance the cholesterol-lowering effect of soy in postmenopausal women. J. Nutr. 2004, 134, 3277–3283. [Google Scholar] [CrossRef] [PubMed]
  56. Chaiyasut, C.; Kesika, P.; Sirilun, S.; Peerajan, S.; Sivamaruthi, B.S. Formulation and evaluation of lactic acid bacteria fermented Brassica juncea (Mustard Greens) pickle with cholesterol lowering property. J. Appl. Pharm. Sci. 2018, 8, 033–042. [Google Scholar]
  57. Davis, H.R.J.; Zhu, L.J.; Hoos, L.M.; Tetzloff, G.; Maguire, M.; Liu, J.; Yao, X.; Iyer, S.P.; Lam, M.H.; Lund, E.G.; et al. Niemann-Pick C1 Like 1 (NPC1L1) is the intestinal phytosterol and cholesterol transporter and a key modulator of whole-body cholesterol homeostasis. J. Biol. Chem. 2004, 279, 33586–33592. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  58. Jia, L.; Betters, J.L.; Yu, L. Niemann-pick C1-like 1 (NPC1L1) protein in intestinal and hepatic cholesterol transport. Annu. Rev. Physiol. 2011, 73, 239–259. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  59. Nashimoto, S.; Yagi, S.; Takeda, N.; Nonaka, M.; Takekuma, Y.; Sugawara, M.; Sato, Y. A new system to evaluate characteristics of Niemann-Pick C1 Like 1-mediated cholesterol transport using Xenopus laevis oocytes. Biochim. Biophys. Acta Biomembr. 2021, 1863, 183508. [Google Scholar] [CrossRef]
  60. Sanders, M.E.; Akkermans, L.M.; Haller, D.; Hammerman, C.; Heimbach, J.; Hörmannsperger, G.; Huys, G.; Levy, D.D.; Lutgendorff, F.; Mack, D.; et al. Safety assessment of probiotics for human use. Gut Microbes 2010, 1, 164–185. [Google Scholar] [CrossRef] [PubMed]
  61. Pradhan, D.; Mallappa, R.H.; Grover, S. Comprehensive approaches for assessing the safety of probiotic bacteria. Food Control. 2020, 108, 106872. [Google Scholar] [CrossRef]
  62. Watanabe, M.; Houten, S.M.; Wang, L.; Moschetta, A.; Mangelsdorf, D.J.; Heyman, R.A.; Moore, D.D.; Auwerx, J. Bile acids lower triglyceride levels via a pathway involving FXR, SHP, and SREBP-1c. J. Clin. Investig. 2004, 113, 1408–1418. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  63. Watanabe, M.; Houten, S.M.; Mataki, C.; Christoffolete, M.A.; Kim, B.W.; Sato, H.; Messaddeq, N.; Harney, J.W.; Ezaki, O.; Kodama, T.; et al. Bile acids induce energy expenditure by promoting intracellular thyroid hormone activation. Nature 2006, 439, 484–489. [Google Scholar] [CrossRef] [PubMed]
  64. Chen, D.; Li, Y.; Sun, H.; Xiao, M.; Zhang, R.L.; Qiu, L.; Tan, B.; Qian, J.M. Correlation between Vitamin D status and gut microbiota in patients with inflammatory bowel disease. Zhongguo Yi Xue Ke Xue Yuan Xue Bao 2020, 42, 740–748. [Google Scholar] [PubMed]
  65. Kanda, N.; Hoashi, T.; Saeki, H. Nutrition and atopic dermatitis. J. Nippon. Med. Sch. 2021, 88, 171–177. [Google Scholar] [CrossRef] [PubMed]
  66. Fukushima, M.; Nakano, M. Effects of a mixture of organisms, Lactobacillus acidophilus or Streptococcus faecalis on cholesterol metabolism in rats fed on a fat- and cholesterol-enriched diet. Br. J. Nutr. 1996, 76, 857–867. [Google Scholar] [CrossRef] [PubMed] [Green Version]
  67. Park, Y.H.; Kim, J.G.; Shin, Y.W.; Kim, S.H.; Whang, K.Y. Effect of dietary inclusion of Lactobacillus acidophilus ATCC 43121 on cholesterol metabolism in rats. J. Microbiol. Biotechnol. 2007, 17, 655–662. [Google Scholar] [PubMed]
  68. Jeun, J.; Kim, S.; Cho, S.Y.; Jun, H.J.; Park, H.J.; Seo, J.G.; Chung, M.J.; Lee, S.J. Hypocholesterolemic effects of Lactobacillus plantarum KCTC3928 by increased bile acid excretion in C57BL/6 mice. Nutrition 2010, 26, 321–330. [Google Scholar] [CrossRef]
  69. Lew, L.C.; Choi, S.B.; Khoo, B.Y.; Sreenivasan, S.; Ong, K.L.; Liong, M.T. Lactobacillus plantarum DR7 Reduces Cholesterol via Phosphorylation of AMPK That Down-regulated the mRNA Expression of HMG-CoA Reductase. Korean J. Food Sci. Anim. Resour. 2018, 38, 350–361. [Google Scholar] [PubMed]
  70. Castelli, V.; d’Angelo, M.; Quintiliani, M.; Benedetti, E.; Cifone, M.G.; Cimini, A. The emerging role of probiotics in neurodegenerative diseases: New hope for Parkinson’s disease? Neural Regen. Res. 2021, 16, 628–634. [Google Scholar]
  71. Sencio, V.; Machado, M.G.; Trottein, F. The lung-gut axis during viral respiratory infections: The impact of gut dysbiosis on secondary disease outcomes. Mucosal Immunol. 2021, 14, 296–304. [Google Scholar] [CrossRef] [PubMed]
Applsci 11 06913 g001 550
Figure 1. The PRISMA flow chart represents the selection criteria to review the effects of probiotics in hypercholesterolemic subjects.
Figure 1. The PRISMA flow chart represents the selection criteria to review the effects of probiotics in hypercholesterolemic subjects.
Applsci 11 06913 g001
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Sivamaruthi, B.S.; Bharathi, M.; Kesika, P.; Suganthy, N.; Chaiyasut, C. The Administration of Probiotics against Hypercholesterolemia: A Systematic Review. Appl. Sci. 2021, 11, 6913. https://doi.org/10.3390/app11156913

AMA Style

Sivamaruthi BS, Bharathi M, Kesika P, Suganthy N, Chaiyasut C. The Administration of Probiotics against Hypercholesterolemia: A Systematic Review. Applied Sciences. 2021; 11(15):6913. https://doi.org/10.3390/app11156913

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Sivamaruthi, Bhagavathi Sundaram, Muruganantham Bharathi, Periyanaina Kesika, Natarajan Suganthy, and Chaiyavat Chaiyasut. 2021. "The Administration of Probiotics against Hypercholesterolemia: A Systematic Review" Applied Sciences 11, no. 15: 6913. https://doi.org/10.3390/app11156913

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