Studies of High Molybdenum-Induced Copper Deprivation in P. przewalskii on the Qinghai Lake Pasture in China
Abstract
:1. Introduction
2. Materials and Methods
2.1. Studied Pasture
2.2. Experimental Design
2.3. Collection Samples
2.4. Analysis and Treatment of Samples
2.5. Statistical Analyses
3. The Results
3.1. Mineral Concentrations
3.2. Physiological Parameter and Biochemical Indexes in Blood
3.3. Mineral Levels in Animal Blood
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Chi, Y.K.; Huo, B.; Shen, X.Y. Distribution characteristics of selenium nutrition on the natural habitat of Przewalski’s gazelle. Pol. J. Environ. Stud. 2019, 29, 67–77. [Google Scholar] [CrossRef] [Green Version]
- Hu, J.; Jiang, Z. Climate change hastens the conservation urgency of an endangered ungulate. PLoS ONE 2011, 6, e22873. [Google Scholar] [CrossRef] [PubMed]
- Song, X.Y.; Xu, Z.M.; Qi, Y.; Yin, X.J.; Ge, J.S. Study of spatial target and compensation standard of eco-compensation in Qinghai lake watershed. J. Glaciol. Geocryol. 2013, 35, 496–503. [Google Scholar] [CrossRef]
- Shen, X.Y.; Huo, B.; Wu, T.; Song, C.; Chi, Y.K. iTRAQ-based proteomic analysis to identify molecular mechanisms of the selenium deficiency response in the Przewalski’s gazelle. J. Proteom. 2019, 203, 103389. [Google Scholar] [CrossRef]
- Shen, X.Y.; Jiang, Z.G. Serum biochemical values and mineral contents of tissues in Przewalski’s and Tibetan gazelles. Afr. J. Biotechnol. 2012, 11, 718–723. [Google Scholar] [CrossRef]
- Huo, B. Response Mechanism of Przewalski’s Gazelle (Procapra przewalskii) to Selenium Deprived Environment; Southwest University of Science and Technology: Mianyang, China, 2020; pp. 3–12. [Google Scholar]
- Feng, H.; Squires, V.R. Socio-environmental dynamics of alpine grasslands, steppes and meadows of the Qinghai–Tibetan Plateau, China: A commentary. Appl. Sci. 2020, 10, 6488. [Google Scholar] [CrossRef]
- Song, C.J.; Jiang, Q.; Shen, X.Y. Responses of Przewalski’s gazelle (P. przewalskii) to zinc nutrition in physical habitat. Biol. Trace Elem. Res. 2021, 199, 142–147. [Google Scholar] [CrossRef] [PubMed]
- Sun, J.Q.; Lu, F.G.; Wu, Y.L. Survey and research on the habitat of bird and Qinghai Lake Procapra przewalskii. Qinghai Prataulture 2011, 20, 41–48. [Google Scholar]
- Cromwell, G.L.; Monegue, H.J.; Stahly, T.S. Long-term effects of feeding a high copper diet to sows during gestation and lactation. J. Anim. Sci. 1993, 71, 2996–3002. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Liu, Z.P.; Ma, Z.; Zhang, Y.J.; Zong-Ping, L.; Zhuo, M.; You-Jia, Z. Studies on the relationship between sway disease of bactrian camels and copper status in Gansu province. Vet. Res. Commun. 1994, 18, 251–260. [Google Scholar] [CrossRef]
- Wu, T.; Song, M.L.; Shen, X.Y. Seasonal dynamics of copper deficiency in Wumeng semi-fine wool sheep. Biol. Trace Elem. Res. 2020, 197, 487–494. [Google Scholar] [CrossRef] [PubMed]
- Tiffany, M.E.; McDowell, L.R.; O’Connor, G.A.; Martin, F.G.; Wilkinson, N.S.; Percival, S.S.; Rabiansky, P.A. Effects of residual and reapplied biosolids on performance and mineral status of grazing beef steers. J. Anim. Sci. 2002, 80, 260–269. [Google Scholar] [CrossRef]
- Shen, X.Y.; Min, X.Y.; Zhang, S.H.; Song, C.J.; Xiong, K.N. Effect of heavy metal contamination in environment on antioxidant function in Wumeng semi-fine wool sheep in the Southwest China. Biol. Trace Elem. Res. 2020, 198, 505–514. [Google Scholar] [CrossRef]
- Malešević, T.P.; Dulić, T.; Obreht, I.; Trivunović, Z.; Marković, R.; Kostić, B.; Važić, T.; Meriluoto, J.; Svirčev, Z. Cyanobacterial Potential for Restoration of Loess Surfaces through Artificially Induced Biocrusts. Appl. Sci. 2020, 11, 66. [Google Scholar] [CrossRef]
- Chi, Y.K.; Zhang, Z.Z.; Song, C.J.; Shen, X.Y. Effects of fertilization on physiological and biochemical parameters of Wumeng sheep in China’s Wumeng prairie. Pol. J. Environ. Stud. 2019, 29, 79–85. [Google Scholar] [CrossRef]
- Dick, A.T. Preliminary observations on the effect of high intakes of molybdenum and of inorganic sulphate on blood copper and on fleece character in crossbred sheep. Aust. Vet. J. 2010, 30, 196–202. [Google Scholar] [CrossRef]
- Xue, Y.F.; Hao, L.Z.; Liu, S.J.; Zhao, S.N. Research progress and application of copper in ruminants. Heilongjiang Anim. Sci. Vet. Med. 2016, 7, 54–58. [Google Scholar] [CrossRef]
- Maihemuti, S.K.D.E.; Gao, C. The situation of research about lacking of copper and animal disenses. Grass-Feed. Livest. 2008, 138, 40–45. [Google Scholar] [CrossRef]
- Xiao, J.; Cui, H.M.; Yang, F.; Peng, X.; Cui, W.; Cheng, A.C.; Chen, T.; Bai, C.M. Effect of high molybdenum on the pathologic lesion and antioxidative function of kidney in broilers. Chin. J. Anim. Vet. Sci. 2010, 41, 1598–1604. [Google Scholar] [CrossRef] [Green Version]
- Lin, L. Study on Injury of Kidney Caused by High Molybdenum and Its Mechanism in Mice; Northwest A&F University: Yangling, China, 2012; pp. 1–43. [Google Scholar]
- Ryssen, J.B.J.V.; Malsen, S.V.; Barrowman, P.R. Effect of dietary molybdenum and sulphur on the copper status of hypercu-protic sheep after withdrawal of dietary copper. Boletín De La Asociación Médica De Puerto Rico 1986, 69, 4–9. [Google Scholar]
- Wang, H.W.; Zhou, B.H.; Zhang, L.; Zhang, S.; Hu, W.F.; Yang, Z.J. Effect of high molybdenum low copper on the structure and TNF-α expression of kidney in mice. Prog. Vet. Med. 2010, 31, 51–54. [Google Scholar]
- Shen, X.; Li, X.; Zhang, R. Studies of “Unsteady Gait Disease” of the Tibetan Gazelle (Procapra picticaudata). J. Wildl. Dis. 2010, 46, 560–563. [Google Scholar] [CrossRef] [PubMed]
- Wang, Z.Y. Diagnosis and surveillance of Cu deficiency induced with Mo. Chin. J. Vet. Med. 1988, 14, 5–8. [Google Scholar]
- Li, G.H.; He, P.X. Diseases Caused by Trace Elements in Animals; Science and Technology Press: Hefei, China, 1990; pp. 100–206. [Google Scholar]
- Di Rosa, M.; Castrogiovanni, P.; Trovato, F.M.; Malatino, L.; Ravalli, S.; Imbesi, R.; Szychlinska, M.A.; Musumeci, G. Adapted Moderate Training Exercise Decreases the Expression of Ngal in the Rat Kidney: An Immunohistochemical Study. Appl. Sci. 2019, 9, 1041. [Google Scholar] [CrossRef] [Green Version]
- Yang, J.; Wang, X.D. Proteomics in organ dysfunction today: A new way to understand the disease. J. Organ Dysfunct. 2006, 2, 66–67. [Google Scholar] [CrossRef]
- Song, C.J.; Gan, S.Q.; Shen, X.Y. Effects of nano-copper poisoning on immune and antioxidant function in the Wumeng semi-fine wool sheep. Biol. Trace Elem. Res. 2020, 198, 515–520. [Google Scholar] [CrossRef] [PubMed]
- Wang, W.L.; Chakraborty, G.; Chakraborty, B. Predicting the risk of chronic kidney disease (ckd) using machine learning algorithm. Appl. Sci. 2020, 11, 202. [Google Scholar] [CrossRef]
- Lee, J.; Prohaska, J.R.; Thiele, D.J. Essential role for mammalian copper transporter ctr1 in copper homeostasis and embryon-ic development. Proc. Natl. Acad. Sci. USA 2001, 98, 6842–6847. [Google Scholar] [CrossRef] [Green Version]
- Oliveira, C.S.S.; Silva, M.O.D.; Silva, C.E.; Carvalho, G.; Reis, M.A.M. Assessment of protein-rich cheese whey waste stream as a nutrients source for low-cost mixed microbial pha production. Appl. Sci. 2018, 8, 1817. [Google Scholar] [CrossRef] [Green Version]
- Huo, B.; Wu, T.; Song, C.J.; Shen, X.Y. Studies of selenium deficiency in the Wumeng semi-fine wool sheep. Biol. Trace Elem. Res. 2019, 194, 152–158. [Google Scholar] [CrossRef]
- Lucejko, M.; Flisiak, R. Effect of HCV core antigen and RNA clearance during therapy with direct acting antivirals on hepatic stiffness measured with shear wave elastography in patients with chronic viral hepatitis C. Appl. Sci. 2018, 8, 198. [Google Scholar] [CrossRef] [Green Version]
- Ha, H.Y.; Alfulaij, N.; Berry, M.J.; Seale, L.A. From selenium absorption to selenoprotein degradation. Biol. Trace Elem. Res. 2019, 192, 26–37. [Google Scholar] [CrossRef]
- Song, C.J.; Shen, X.Y. Effects of environmental zinc deficiency on antioxidant system function in Wumeng semi-fine wool sheep. Biol. Trace Elem. Res. 2020, 195, 110–116. [Google Scholar] [CrossRef]
- Zhao, K.; Chi, Y.; Shen, X. Studies on Edema Pathema in Hequ Horse in the Qinghai-Tibet Plateau. Biol. Trace Elem. Res. 2020, 198, 142–148. [Google Scholar] [CrossRef]
- Su, X.-Q.; Zhang, G.-J.; Ma, Y.; Chen, M.; Chen, S.-H.; Duan, S.-M.; Wan, J.-Q.; Hashimoto, F.; Lv, H.-P.; Li, J.-H.; et al. Isolation, identification, and biotransformation of teadenol a from solid state fermentation of pu-erh tea and in vitro antioxidant activity. Appl. Sci. 2016, 6, 161. [Google Scholar] [CrossRef] [Green Version]
- Xie, Z.-Z.; Liu, Y.; Bian, J.-S. Hydrogen sulfide and cellular redox homeostasis. Oxidative Med. Cell. Longev. 2016, 2016, 1–12. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Chen, C.-J.; Tsay, P.-K.; Huang, S.-F.; Tsui, P.-H.; Yu, W.-T.; Hsu, T.-H.; Tai, J.; Tai, D.-I. Effects of hepatic steatosis on non-invasive liver fibrosis measurements between hepatitis b and other etiologies. Appl. Sci. 2019, 9, 1961. [Google Scholar] [CrossRef] [Green Version]
- Flueck, W.T.; Smith-Flueck, J.M.; Mionczynski, J.; Mincher, B.J. The implications of selenium deficiency for wild herbivore conservation: A review. Eur. J. Wildl. Res. 2012, 58, 761–780. [Google Scholar] [CrossRef]
- Zhao, K.; Huo, B.; Shen, X.Y. Studies on Antioxidant Capacity in Selenium-Deprived the Choko Yak in the Shouqu Prairie. Biol. Trace Elem. Res. 2020, 1–6. [Google Scholar] [CrossRef]
- Cozza, G.; Rossetto, M.; Bosello-Travain, V.; Maiorino, M.; Roveri, A.; Toppo, S.; Zaccarin, M.; Zennaro, L.; Ursini, F. Glutathione peroxidase 4-catalyzed reduction of lipid hydroperoxides in membranes: The polar head of membrane phospholipids binds the enzyme and addresses the fatty acid hydroperoxide group toward the redox center. Free Radic. Biol. Med. 2017, 112, 1–11. [Google Scholar] [CrossRef] [PubMed]
- Çelebi, Ş. Effect of dietary vitamin E, selenium and their combination on concentration of selenium, MDA, and antioxidant enzyme activities in some tissues of laying hens. Pak. J. Zool. 2019, 51, 1155–1161. [Google Scholar] [CrossRef]
- Huo, B.; Wu, T.; Song, C.; Shen, X. Effects of selenium deficiency in the environment on antioxidant systems of Wumeng semi-fine wool sheep. Pol. J. Environ. Stud. 2020, 29, 1649–1657. [Google Scholar] [CrossRef]
- Shen, X.Y.; Huo, B.; Li, Y.F.; Song, C.J.; Wu, T.; He, J. Response of the critically endangered Przewalski’s gazelle (Procapra przewalskii) to selenium deprived environment. J. Proteomics 2021, 241, 104218. [Google Scholar] [CrossRef] [PubMed]
- Zhao, K.; Min, X.Y.; Shen, X.Y. Response of the Wumeng Sheep to Phosphorus Deprived Environment in the Southwest China. Pol. J. Environ. Stud. 2021, 30, 2927–2934. [Google Scholar] [CrossRef]
Elements | Soils | Forages (DM) | ||
---|---|---|---|---|
Affected | Unaffected | Affected | Unaffected | |
Cu | 14.63 ± 2.41 | 14.75 ± 2.42 | 6.53 ± 0.59 | 6.71 ± 0.66 |
Mo | 4.17 ± 0.20 * | 1.15 ± 0.23 | 5.17 ± 0.15 * | 1.14 ± 0.13 |
Fe | 4247.35 ± 25.97 | 4255.67 ± 23.72 | 335.25 ± 5.36 | 332.65 ± 6.86 |
Mn | 58.97 ± 6.28 | 57.17 ± 5.97 | 12.97 ± 1.25 | 12.65 ± 1.76 |
Co | 5.76 ± 0.86 | 5.68 ± 0.68 | 1.15 ± 0.06 | 1.14 ± 0.07 |
Elements | Blood | Hair | ||
---|---|---|---|---|
Affected | Unaffected | Affected | Unaffected | |
Cu | 0.18 ± 0.03 * | 1.61 ± 0.63 | 3.99 ± 0.33 * | 7.29 ± 0.37 |
Mo | 0.39 ± 0.03 * | 0.23 ± 0.02 | 4.35 ± 0.06 * | 2.49 ± 0.04 |
Fe | 442.67 ± 62.38 | 439.65 ± 63.47 | 318.65 ± 43.26 | 316.75 ± 45.64 |
Mn | 0.53 ± 0.02 | 0.52 ± 0.03 | 11.65 ± 1.58 | 11.87 ± 1.86 |
Co | 0.55 ± 0.03 | 0.54 ± 0.05 | 1.03 ± 0.04 | 1.02 ± 0.03 |
Parameters | Affected Gazelles | Healthy Gazelles |
---|---|---|
Hb (g/L) | 90.76 ± 7.41 * | 119.63 ± 8.87 |
RBC (1012/L) | 5.73 ± 0.33 * | 7.99 ± 0.25 |
PCV (%) | 39.63 ± 3.76 * | 53.63 ± 3.58 |
MCV (fL) | 47.35 ± 3.21 * | 53.47 ± 3.76 |
MCH (pg) | 10.74 ± 1.64 * | 16.38 ± 1.56 |
MCHC (%) | 22.76 ± 1.37 | 24.31 ± 1.57 |
WBC (109/L) | 8.48 ± 0.58 | 9.76 ± 0.52 |
Items | Affected Gazelles | Healthy Gazelles |
---|---|---|
AST (U/L) | 132.47 ± 11.22 * | 89.98 ± 8.37 |
ALT (U/L) | 45.77 ± 4.35 | 44.77 ± 3.57 |
LDH (U/L) | 553.48 ± 34.58 * | 425.73 ± 30.75 |
CPK (U/L) | 386.37 ± 63.86 * | 255.37 ± 54.97 |
ALP (U/L) | 1223.76 ± 207.83 * | 751.28 ± 93.56 |
CR (µmoL/L) | 65.56 ± 6.67 | 66.74 ± 5.27 |
TP (g/L) | 38.63 ± 4.48 * | 56.38 ± 3.86 |
ALB (g/L) | 24.86 ± 3.58 * | 32.86 ± 2.36 |
GLB (g/L) | 13.86 ± 2.49 * | 24.86 ± 2.88 |
A/G | 1.84 ± 0.21 * | 1.29 ± 0.14 |
Chol (mmol/L) | 1.97 ± 0.21 | 1.89 ± 0.23 |
Items | Affected Gazelles | Healthy Gazelles |
---|---|---|
T-AOC (U/mL) | 2.23 ± 0.11 * | 4.75 ± 0.21 |
GSH-Px (U/mL) | 227.54 ± 11.77 * | 351.65 ± 17.36 |
MDA (nmol/L) | 42.48 ± 4.27 * | 13.76 ± 2.26 |
SOD (U/mL) | 58.37 ± 4.52 * | 99.72 ± 9.96 |
CAT (U/mL) | 6.76 ± 0.21 * | 14.36 ± 1.76 |
Elements | Treatment Group | Control Group | ||||
---|---|---|---|---|---|---|
0 d | 10 d | 20 d | 0 d | 10 d | 20 d | |
Cu | 0.17 ± 0.04 * | 1.53 ± 0.17 * | 1.77 ± 0.16 * | 0.17 ± 0.03 | 0.16 ± 0.03 | 0.14 ± 0.04 |
Mo | 0.22 ± 0.02 | 0.19 ± 0.02 | 0.17 ± 0.01 | 0.24 ± 0.03 | 0.22 ± 0.04 | 0.21 ± 0.03 |
Fe | 441.43 ± 61.87 | 443.75 ± 62.38 | 442.86 ± 59.32 | 441.65 ± 60.65 | 439.68 ± 63.48 | 439.65 ± 67.86 |
Mn | 0.53 ± 0.02 | 0.52 ± 0.01 | 0.52 ± 0.02 | 0.54 ± 0.03 | 0.53 ± 0.02 | 0.51 ± 0.02 |
Co | 0.54 ± 0.04 | 0.54 ± 0.04 | 0.56 ± 0.02 | 0.55 ± 0.04 | 0.57 ± 0.03 | 0.56 ± 0.03 |
Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. |
© 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
Share and Cite
Zhai, B.; Zhao, K.; Liu, F.; Shen, X. Studies of High Molybdenum-Induced Copper Deprivation in P. przewalskii on the Qinghai Lake Pasture in China. Appl. Sci. 2021, 11, 5071. https://doi.org/10.3390/app11115071
Zhai B, Zhao K, Liu F, Shen X. Studies of High Molybdenum-Induced Copper Deprivation in P. przewalskii on the Qinghai Lake Pasture in China. Applied Sciences. 2021; 11(11):5071. https://doi.org/10.3390/app11115071
Chicago/Turabian StyleZhai, Bowen, Kui Zhao, Fuyuan Liu, and Xiaoyun Shen. 2021. "Studies of High Molybdenum-Induced Copper Deprivation in P. przewalskii on the Qinghai Lake Pasture in China" Applied Sciences 11, no. 11: 5071. https://doi.org/10.3390/app11115071