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Open AccessFeature PaperArticle

Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells

1
Department of Anatomy, Histology, Forensic-Medicine and Orthopedics, Section of Histology and Embryology, “Sapienza” University of Rome, 00161 Rome, Italy
2
Department of Neuroscience, Imaging and Clinical Sciences and Center for Advanced Studies and Technology (CAST), “G. d’Annunzio” University of Chieti-Pescara, 66100 Chieti, Italy
3
Department of Experimental Medicine, Università degli Studi della Campania “Luigi Vanvitelli”, 80138 Naples, Italy
4
Italian Space Agency (ASI), 00133 Rome, Italy
*
Authors to whom correspondence should be addressed.
Equal contribution as first authors.
Equal contribution as senior authors.
Appl. Sci. 2020, 10(22), 8289; https://doi.org/10.3390/app10228289
Received: 28 October 2020 / Revised: 10 November 2020 / Accepted: 20 November 2020 / Published: 23 November 2020
(This article belongs to the Special Issue Advances in Space Biology: Cell Behavior in Microgravity)
The direct impact of microgravity exposure on male germ cells, as well as on their malignant counterparts, has not been largely studied. In previous works, we reported our findings on a cell line derived from a human seminoma lesion (TCam-2 cell line) showing that acute exposure to simulated microgravity altered microtubule orientation, induced autophagy, and modified cell metabolism stimulating ROS production. Moreover, we demonstrated that the antioxidant administration prevented both TCam-2 microgravity-induced microtubule disorientation and autophagy induction. Herein, expanding previous investigations, we report that simulated microgravity exposure for 24 h induced the appearance, at an ultrastructural level, of cell-to-cell junctional contacts that were not detectable in cells grown at 1 g. In line with this result, pan-cadherin immunofluorescence analyzed by confocal microscopy, revealed the clustering of this marker at the plasma membrane level on microgravity exposed TCam-2 cells. The upregulation of cadherin was confirmed by Western blot analyses. Furthermore, we demonstrated that the microgravity-induced ROS increase was responsible for the distribution of cadherin nearby the plasma membrane, together with beta-catenin since the administration of antioxidants prevented this microgravity-dependent phenomenon. These results shed new light on the microgravity-induced modifications of the cell adhesive behavior and highlight the role of ROS as microgravity activated signal molecules. View Full-Text
Keywords: TCam-2 seminoma cells; microgravity; antioxidant compounds; cell adhesion; mitohormesis TCam-2 seminoma cells; microgravity; antioxidant compounds; cell adhesion; mitohormesis
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MDPI and ACS Style

Catizone, A.; Morabito, C.; Cammarota, M.; Schiraldi, C.; Corano Scheri, K.; Ferranti, F.; Mariggiò, M.A.; Ricci, G. Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells. Appl. Sci. 2020, 10, 8289. https://doi.org/10.3390/app10228289

AMA Style

Catizone A, Morabito C, Cammarota M, Schiraldi C, Corano Scheri K, Ferranti F, Mariggiò MA, Ricci G. Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells. Applied Sciences. 2020; 10(22):8289. https://doi.org/10.3390/app10228289

Chicago/Turabian Style

Catizone, Angela; Morabito, Caterina; Cammarota, Marcella; Schiraldi, Chiara; Corano Scheri, Katia; Ferranti, Francesca; Mariggiò, Maria A.; Ricci, Giulia. 2020. "Microgravity-Induced Cell-to-Cell Junctional Contacts Are Counteracted by Antioxidant Compounds in TCam-2 Seminoma Cells" Appl. Sci. 10, no. 22: 8289. https://doi.org/10.3390/app10228289

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