A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Ethics Statement
2.2. Animals and Data Collection
2.3. DNA Extraction and DNA Pool Construction
2.4. PCR Amplification and Sequenci
2.5. Genotyping
2.6. Statistical Analysis
3. Results
3.1. Sequence Variants Identified in the Donkey NR6A1 Gene
3.2. Polymorphisms and Genetic Diversity
3.3. Association Analysis of Polymorphisms with Growth Traits of the Donkey
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Locus | Breeds | N | Genotypic Frequencies | Allelic Frequencies | HWE | Population Parameters | ||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
II | ID | DD | I | D | p | Ho | He | Ne | PIC | |||
NR6A1 (NW-014637488) | DZ | 346 | 0.84 (n = 291) | 0.15 (n = 53) | 0.01 (n = 2) | 0.92 | 0.08 | 0.81 | 0.849 | 0.151 | 1.178 | 0.14 |
GZ | 62 | 0.82 (n = 51) | 0.16 (n = 10) | 0.02 (n = 1) | 0.90 | 0.10 | 0.54 | 0.825 | 0.175 | 1.212 | 0.16 |
Locus | Types | Breeds | DZ | GZ |
---|---|---|---|---|
NR6A1 (NW-014637488) | Genotypic frequencies | DZ | – | χ2 = 0.81 |
GZ | (p > 0.05) | – | ||
Allelic frequencies | DZ | – | χ2 = 0.06 | |
GZ | (p > 0.05) | – |
Locus | Breeds | Growth Traits | Observed Genotypes (LSM a ± SE) | p | ||
---|---|---|---|---|---|---|
II | ID | DD | ||||
NR6A1 (NW-014637488) | DZ | Body height | 136.56 ± 6.94 a (n = 291) | 134.98 ± 5.11 b (n = 53) | 133.00 ± 7.07 (n = 2) | 0.019 |
Body length | 135.78 ± 8.17 a (n = 291) | 135.32 ± 6.38 b (n = 53) | 133.00 ± 5.65 (n = 2) | 0.025 | ||
Chest circumference | 148.66 ± 8.89 a (n = 291) | 148.11 ± 6.90 b (n = 53) | 157.75 ± 10.96 (n = 2) | 0.048 | ||
Chest depth | 55.26 ± 3.86 a (n = 291) | 54.83 ± 2.61 b (n = 53) | 56.00 ± 4.24 (n = 2) | 0.002 | ||
GZ | Body height | 135.68 ± 6.77 a (n = 51) | 133.10 ± 4.53 b (n = 10) | 0 | 0.048 | |
Body length | 128.77 ± 7.07 a (n = 51) | 125.70 ± 5.27 a (n = 10) | 0 | 0.467 |
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Share and Cite
Fang, X.; Lai, Z.; Liu, J.; Zhang, C.; Li, S.; Wu, F.; Zhou, Z.; Lei, C.; Dang, R. A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys. Animals 2019, 9, 681. https://doi.org/10.3390/ani9090681
Fang X, Lai Z, Liu J, Zhang C, Li S, Wu F, Zhou Z, Lei C, Dang R. A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys. Animals. 2019; 9(9):681. https://doi.org/10.3390/ani9090681
Chicago/Turabian StyleFang, Xiya, Zhenyu Lai, Jie Liu, Chunlan Zhang, Shipeng Li, Fei Wu, Zihui Zhou, Chuzhao Lei, and Ruihua Dang. 2019. "A Novel 13 bp Deletion within the NR6A1 Gene Is Significantly Associated with Growth Traits in Donkeys" Animals 9, no. 9: 681. https://doi.org/10.3390/ani9090681