Transcriptome Analysis of the Effects of Fasting Caecotrophy on Hepatic Lipid Metabolism in New Zealand Rabbits
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals and Experimental Design
2.1.1. Ethics Statement
2.1.2. Animals
2.1.3. Sample Collection
2.1.4. Histology
2.1.5. Serum Biochemical Indices
2.2. Transcriptome Analysis
2.2.1. Liver Transcriptome Library Construction and Illumina Sequencing
2.2.2. Data Analysis
2.3. RT-qPCR
2.4. Data Analysis
3. Results
3.1. Effects of Wearing a Collar on Rabbit Growth
3.2. Body Weight and Liver Histology Changes
3.3. Serum Biochemistry
3.4. Liver Transcriptomic Analysis
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Data Availability Statement
References
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Items | Groups | |||
---|---|---|---|---|
CON | CA | CP | ||
IBW (g) | 1 300.42 ± 128.02 | 1 288.75 ± 129.91 | 1 273.54 ± 116.05 | |
ADFI (g) | Total Feed intake/g | 143.98 ± 10.32 | 140.48 ± 10.01 | 138.60 ± 7.87 |
Feed intake at daylight/g | 48.12 ± 8.41 | 43.21 ± 3.99 | 47.29 ± 4.64 | |
Feed intake at night/g | 95.86 ± 8.49 | 97.27 ± 10.93 | 91.40 ± 5.76 | |
ADG (g) | 21.60 ± 3.52 a | 21.69 ± 2.11 a | 19.64 ± 1.98 b | |
Final weight (g) | 2 736.36 ± 271.13 a | 2 722.00 ± 165.15 a | 2 524.76 ± 211.59 b | |
F/G | 6.80 ± 1.08 | 6.52 ± 0.61 | 7.12 ± 0.75 |
Items | Treatment | |
---|---|---|
Control Group | Experimental Group | |
Initial weight (kg) | 0.913 ± 0.118 | 0.922 ± 0.163 |
Final weight (kg) | 2.935 ± 1.188 a | 2.292 ± 0.170 b |
Weight gain (%) | 2.021 ± 0.159 a | 1.370 ± 0.262 b |
SGR (%day-1) | 0.033 ± 0.002 a | 0.022 ± 0.004 b |
FCR | 3.555 ± 0.341 a | 4.795 ± 1.238 b |
HIS (%) | 44.702 ± 4.918 | 45.971 ± 3.971 |
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Wang, Y.; Xu, H.; Sun, G.; Xue, M.; Sun, S.; Huang, T.; Zhou, J.; Loor, J.J.; Li, M. Transcriptome Analysis of the Effects of Fasting Caecotrophy on Hepatic Lipid Metabolism in New Zealand Rabbits. Animals 2019, 9, 648. https://doi.org/10.3390/ani9090648
Wang Y, Xu H, Sun G, Xue M, Sun S, Huang T, Zhou J, Loor JJ, Li M. Transcriptome Analysis of the Effects of Fasting Caecotrophy on Hepatic Lipid Metabolism in New Zealand Rabbits. Animals. 2019; 9(9):648. https://doi.org/10.3390/ani9090648
Chicago/Turabian StyleWang, Yadong, Huifen Xu, Guirong Sun, Mingming Xue, Shuaijie Sun, Tao Huang, Jianshe Zhou, Juan J. Loor, and Ming Li. 2019. "Transcriptome Analysis of the Effects of Fasting Caecotrophy on Hepatic Lipid Metabolism in New Zealand Rabbits" Animals 9, no. 9: 648. https://doi.org/10.3390/ani9090648