Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Fish
2.2. Isolation and Identification of Bacteria
2.3. Antibiotic Susceptibility Testing
2.4. Experimental Infections
3. Results
3.1. Clinical Signs and Isolation of Bacteria from Diseased Common Carp
3.2. Phylogenetic Tree and Virulence Related Genes
3.3. Experimental Infections
3.4. Determination of Antimicrobial Resistance
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Group | Concentration (CFU) | Fish | Dead Fish Number on Day After Challenge | Accumulative Mortality | LD50 Value (CFU) | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | |||||
Control | 0 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0% | |
P. shigelloides | 1.0 × 107 | 10 | 0 | 0 | 0 | 0 | 4 | 1 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 100% | 4.74 × 104 |
1.0 × 106 | 10 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 3 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 90% | ||
1.0 × 105 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 70% | ||
1.0 × 104 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 20% | ||
C. freundii | 1.0 × 107 | 10 | 0 | 0 | 0 | 0 | 4 | 2 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 100% | 1.95 × 104 |
1.0 × 106 | 10 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 3 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 100% | ||
1.0 × 105 | 10 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 5 | 1 | 1 | 0 | 0 | 0 | 0 | 90% | ||
1.0 × 104 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 30% | ||
A. veronii | 1.0 × 107 | 10 | 2 | 4 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 90% | 5.12 × 104 |
1.0 × 106 | 10 | 1 | 0 | 0 | 0 | 4 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 90% | ||
1.0 × 105 | 10 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 2 | 0 | 0 | 0 | 70% | ||
1.0 × 104 | 10 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 20% | ||
A. hydrophila | 1.0 × 107 | 10 | 1 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 100% | 1.53 × 105 |
1.0 × 106 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 4 | 0 | 1 | 0 | 0 | 0 | 0 | 80% | ||
1.0 × 105 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 0 | 0 | 0 | 40% | ||
1.0 × 104 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 10% | ||
Mixed infection | 1.0 × 107 | 10 | 0 | 0 | 0 | 0 | 3 | 1 | 1 | 2 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 100% | 5.41 × 104 |
1.0 × 106 | 10 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 2 | 1 | 2 | 0 | 1 | 0 | 0 | 90% | ||
1.0 × 105 | 10 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 1 | 0 | 50% | ||
1.0 × 104 | 10 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 30% |
Antibiotic | Drug Concentration (μg/disc) | Inhibition Zone Diameter (mm) | |||
---|---|---|---|---|---|
A. veronii | A. hydrophila | C. freundii | P. shigelloides | ||
Streptomycin | 10 | 19 S | 15 S | 15 S | 15 S |
Enrofloxacin | 10 | 40 S | 23 S | 10 R | 30 S |
Florfenicol | 30 | 30 S | 32 S | 0 R | 28 S |
Gentamicin | 10 | 21 S | 19 S | 20 S | 19 S |
Kanamycin | 30 | 20 S | 20 S | 15 I | 18 S |
Neomycin | 30 | 17 S | 18 S | 17 I | 18 S |
Tetracycline | 30 | 11 R | 25 S | 0 R | 21 S |
Norfloxacin | 10 | 34 S | 23 S | 14 I | 27 S |
Co-trimoxazole | 23.75/1.25 | 19 S | 20 S | 0 R | 13 I |
Ceftizoxime | 30 | 44 S | 40 S | 32 S | 37 S |
Ampicillin | 10 | 10 R | 10 R | 14 I | 0 R |
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Zhang, J.; Qiao, D.; Wang, H.; Zhao, X.; Jiang, X.; Zhu, L.; Zhang, J.; Li, L.; Kong, X.; Pei, C. Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii. Animals 2025, 15, 805. https://doi.org/10.3390/ani15060805
Zhang J, Qiao D, Wang H, Zhao X, Jiang X, Zhu L, Zhang J, Li L, Kong X, Pei C. Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii. Animals. 2025; 15(6):805. https://doi.org/10.3390/ani15060805
Chicago/Turabian StyleZhang, Jinghang, Dan Qiao, Haoyu Wang, Xianliang Zhao, Xinyu Jiang, Lei Zhu, Jie Zhang, Li Li, Xianghui Kong, and Chao Pei. 2025. "Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii" Animals 15, no. 6: 805. https://doi.org/10.3390/ani15060805
APA StyleZhang, J., Qiao, D., Wang, H., Zhao, X., Jiang, X., Zhu, L., Zhang, J., Li, L., Kong, X., & Pei, C. (2025). Mixed Infection in Common Carp (Cyprinus carpio) Caused by Aeromonas veronii, Aeromonas hydrophila, Plesiomonas shigelloides, and Citrobacter freundii. Animals, 15(6), 805. https://doi.org/10.3390/ani15060805