Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle–Yak
Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animal and Sample Collection
2.2. Tissue Processing
2.3. Immunohistochemistry
2.4. Semi-Quantification of Immunolabelling in Epididymis Epithelial Cells
2.5. Statistical Analysis
3. Results
3.1. AR Expression in Epididymal Epithelial Cells During Yak Development
3.2. AR Expression in Epididymal Epithelial Cells of Mature Yak and Cattle–Yak
3.3. SRD5A2 Expression in Epididymal Epithelial Cells During Yak Development
3.4. SRD5A2 Expression in Epididymal Epithelial Cells of Mature Yak and Cattle–Yak
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
AR | androgen receptor |
SRD5A1 | 5α-reductase isoform 1 |
SRD5A2 | 5α-reductase isoform 2 |
F1 | first generation |
F2 | second generation |
miRNAs | microRNAs |
DHT | 5α-dihydroxytestosterone |
H2O2 | hydrogen peroxide |
PBS | phosphate-buffered saline |
IgG | immunoglobulin G |
BSA | bovine serum albumin |
AEC | 3-amino-9-ethylcarbazol |
ANOVA | analysis of variance |
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Wittayarat, M.; Kawanishi, K.; Ohata, H.; Nagahara, M.; Sambuu, R.; Sambuu, O.; Hirata, M.; Tanihara, F.; Taniguchi, M.; Otoi, T.; et al. Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle–Yak. Animals 2025, 15, 660. https://doi.org/10.3390/ani15050660
Wittayarat M, Kawanishi K, Ohata H, Nagahara M, Sambuu R, Sambuu O, Hirata M, Tanihara F, Taniguchi M, Otoi T, et al. Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle–Yak. Animals. 2025; 15(5):660. https://doi.org/10.3390/ani15050660
Chicago/Turabian StyleWittayarat, Manita, Kimika Kawanishi, Haruka Ohata, Megumi Nagahara, Rentsenkhand Sambuu, Otgonjargal Sambuu, Maki Hirata, Fuminori Tanihara, Masayasu Taniguchi, Takeshige Otoi, and et al. 2025. "Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle–Yak" Animals 15, no. 5: 660. https://doi.org/10.3390/ani15050660
APA StyleWittayarat, M., Kawanishi, K., Ohata, H., Nagahara, M., Sambuu, R., Sambuu, O., Hirata, M., Tanihara, F., Taniguchi, M., Otoi, T., & Sato, Y. (2025). Aberrant Expression Levels of Androgen Receptor and SRD5A2 in Epididymal Epithelial Cells of Crossbred Infertile Cattle–Yak. Animals, 15(5), 660. https://doi.org/10.3390/ani15050660